Abstract: This invention relates to a cell expansion system and to a method of determining when to harvest cells from the cell expansion system.

Abstract: Cell expansion systems and methods of use are provided. The cell expansion systems generally include a hollow fiber cell growth chamber, and first and second circulation loops (intracapillary loops and extracapillary loops) associated with the interior of the hollow fibers and exterior of the hollow fibers, respectively. Detachable flow circuits and methods of expanding cells are also provided.

Abstract: Method for separating a volume of composite liquid into at least a first component and a second component including centrifuging a separation bag containing a volume of composite liquid and a volume of gas so as to sediment at least a first component and a second component separation bag; displacing a volume of hydraulic fluid against the separation bag to cause a transfer of at least one fraction of the content of the separation bag into at least one component bag connected to the separation bag; determining the volume of hydraulic fluid displaced and determining the volume of the composite liquid or the transferred components from the determined volume of displaced hydraulic fluid.