Patents Represented by Attorney, Agent or Law Firm Emanuel J. Vacchiano
  • System for pH-neutral stable electrophoresis gel
    Patent number: 7422670
    Abstract: A gel and buffer system for gel electrophoresis wherein separation occurs at neutral pH.
    Type: Grant
    Filed: February 21, 2003
    Date of Patent: September 9, 2008
    Inventors: Timothy V Updyke, Sheldon C Engelhorn
  • Methods, articles and kits for labeling polymer gels
    Patent number: 7361260
    Abstract: The invention provides methods, apparatus and kits for integrally labeling polymeric gels, such as polyacrylamide and agarose gels, with indicia that can serve, inter alia, unambiguously to identify the individual gel, using polymeric film labeled with detectable indicia and coated with materials that bond to the gel matrix as the gel polymerizes.
    Type: Grant
    Filed: September 20, 2002
    Date of Patent: April 22, 2008
    Inventors: Joseph W Amshey, Allen Bautista
  • Method for identifying an agent that affects a hedgehog signaling pathway
    Patent number: 6733971
    Abstract: The present invention provides transcription factors associated with the hedgehog signaling pathway that are regulated by dephosphorylation by phosphatases. Hedgehog response elements (HRE) that interact with the dephosphorylated transcription factors are also provided as well as methods for identifying compounds that are phosphatase inhibitors. Methods of treating tumors in a subject by modulating the phosphorylation of the transcription factor are also included.
    Type: Grant
    Filed: August 21, 2001
    Date of Patent: May 11, 2004
    Assignees: Baylor College of Medicine, The Johns Hopkins University School of Medicine
    Inventors: Philip A. Beachy, Ming-Jer Tsai, Sophia Tsai, Venkatesh Krishnan, Chien-Huan Chen
  • Method for detection of biological factors in epidermis
    Patent number: 6720145
    Abstract: Disclosed is a method for removing polynucleotide from the skin. This polynucleotide can be used to detect dermatitis and distinguish an irritant reaction from an allergic reaction by characterizing the polynucleotide according to the polypeptide which it encodes. Additionally, provided are methods for non-invasive isolation of samples from the skin as well as kits for use in the methods provided herein.
    Type: Grant
    Filed: September 28, 2001
    Date of Patent: April 13, 2004
    Assignee: Dermtech International
    Inventors: Lawrence A. Rheins, Vera B. Morhenn
  • Methods of diagnosing and treating hepatic cell proliferative disorders
    Patent number: 6709818
    Abstract: Provided are methods and compositions useful for the diagnosis, prognosis and treatment of hepatic cellular proliferative disorders. The methods include the modulation or analysis of hypemethylated glutathione-S-transferase nuleic acid sequence in hepatic samples and biological fluids.
    Type: Grant
    Filed: October 12, 2000
    Date of Patent: March 23, 2004
    Assignee: The Johns Hopkins University School of Medicine
    Inventors: William G. Nelson, Xiaohui Lin, Julia C. Tchou, Jila Bakker
  • Growth differentiation factor, lefty-2
    Patent number: 6635480
    Abstract: Growth differentiation factor, Lefty-2, is disclosed along with its polynucleotide sequence and amino acid sequence. Also disclosed are diagnostic and therapeutic methods of using the Lefty-2 polypeptide and polynucleotide sequences.
    Type: Grant
    Filed: April 14, 2000
    Date of Patent: October 21, 2003
    Assignee: The Johns Hopkins University School of Medicine
    Inventors: Se-Jin Lee, Thanh Huynh, Suzanne Sebald
  • Modified nucleotides and methods useful for nucleic acid sequencing
    Patent number: 6579704
    Abstract: The present invention relates to a method for sequencing nucleic acids. In particular, such method includes incorporating certain modified nucleotides referred to as Simtides into nucleic acid strands. These Simtides are complexes comprising a nucleotide base (or analog thereof), a linker, and a label capable of generating a detectable signal for sequencing. A Simtide may be incorporated into a sequencing fragment as a primer component, as a chain elongator, or as a chain terminator.
    Type: Grant
    Filed: May 8, 2001
    Date of Patent: June 17, 2003
    Assignee: Invitrogen Corporation
    Inventor: Jay M. Short
  • Method of detecting a nucleic acid
    Patent number: 6562567
    Abstract: Disclosed is a method of detecting specific nucleic acids using an oligonucleotide linked to a cleavable tag. The presence of a specific nucleic acid in a population of nucleic acids is determined by hybridizing an oligonucleotide containing the tag to a population of nucleic acids, separating hybridizing bound oligonucleotides, and then removing and identifying the tag. Also provided are compositions and kits comprising oligonucleotides linked to a cleavable tag.
    Type: Grant
    Filed: January 27, 1999
    Date of Patent: May 13, 2003
    Assignee: California Institute of Technology
    Inventor: Barbara J. Wold
  • Detectably and removably tagged nucleic acids
    Patent number: 6544738
    Abstract: Disclosed is a method of detecting specific nucleic acids using an oligonucleotide linked to a cleavable tag. The presence of a specific nucleic acid in a population of nucleic acids is determined by hybridizing an oligonucleotide containing the tag to a population of nucleic acids, separating hybridizing bound oligonucleotides, and then removing and identifying the tag. Also provided are compositions and kits comprising oligonucleotides linked to a cleavable tag.
    Type: Grant
    Filed: April 25, 2000
    Date of Patent: April 8, 2003
    Assignee: California Institute of Technology
    Inventor: Barbara J. Wold