Patents Represented by Attorney, Agent or Law Firm Eugene Moroz
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Patent number: 6709821Abstract: Novel polypeptides having functional domains of interest are described, along with DNA sequences that encode the same. A method of identifying these polypeptides by means of a sequence-independent (that is, independent of the primary sequence of the polypeptide sought), recognition unit-based functional screen is also disclosed. Various applications of the method and of the polypeptides identified are described, including their use in assay kits for drug discovery, modification, and refinement.Type: GrantFiled: June 13, 2001Date of Patent: March 23, 2004Assignees: University of North Carolina at Chapel Hill, Cytogen Corp.Inventors: Andrew B. Sparks, Noah Hoffman, Brian K. Kay, Dana M. Fowlkes, Stephen J. McConnell
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Patent number: 6703482Abstract: Peptides having general and specific binding affinities for the Src homology region 3 (SH3) domains of proteins are disclosed in the present invention. In particular, SH3 binding peptides have been isolated from three phage-displayed random peptide libraries which had been screened for isolates that bind to bacterial fusion proteins of SH3 domains and glutathione S-transferase (GST). Preferred peptides are disclosed having a core 7-mer sequence (preferably, a consensus motif) and two or more, preferably at least six, additional amino acid residues flanking the core sequence, for a total length of 9, preferably at least 13, amino acid residues and no more than about 45 amino acid residues. Such peptides manifest preferential binding affinities for certain SH3 domains. The preferred peptides exhibit specific binding affinities for the Src-family of proteins. In vitro and in vivo results are presented which demonstrate the biochemical activity of such peptides.Type: GrantFiled: August 23, 2001Date of Patent: March 9, 2004Assignee: The University of North Carolina at Chapel HillInventors: Brian K. Kay, Andrew B. Sparks, Judith M. Thorn, Lawrence A. Quilliam, Channing J. Der
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Patent number: 6686151Abstract: The present invention provides a hybridization method and kit for the detection and measurement of biological molecules. A test sample containing the biological molecules of interest is hybridized with an unlabeled or optionally a detectably labeled complementary biomolecule to form a double-stranded hybrid immobilized to a solid phase. The immobilized hybrid may be detected with an entity which specifically recognizes an RNA:DNA hybrid, followed by analyses and quantification. Therefore, the present invention provides a method and kit to detect and measure biological molecules that is simple to use, highly specific, sensitive, and accurate for screening a plurality of biological molecules.Type: GrantFiled: November 6, 2000Date of Patent: February 3, 2004Assignee: Digene CorporationInventors: James G. Lazar, Joan M. Zakel, Christina M. Strange, Inna R. Williams, Attila T. Lorincz, Abel De La Rosa
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Patent number: 5637703Abstract: The invention concerns chemical compounds, derivatives of genistein, which exhibit pronounced immunosuppressant and antitumor activity. In particular, the invention concerns compounds having general formula 1, where R.sup.1, R.sup.2, R.sup.3 are the same or different, R.sup.1 denotes hydrogen or alkyl, R.sup.2 and R.sup.3 are the same or different and denote hydrogen, alkyl or aryl, or R.sup.2 and R.sup.3 are taken together with the nitrogen to which they are connected to form a heterocycle ring.Type: GrantFiled: August 13, 1996Date of Patent: June 10, 1997Assignee: Drug InstituteInventors: Aleksander P. Mazurek, Krzysztof Biniecki, Lech Kozerski
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Patent number: 5444105Abstract: A composition is disclosed for transferring and adhering tape-mounted hisogical sections, to specimen mounting surfaces, usually glass microscope slides to expedite and simplify the safe removal of the mounting tape and the subsequent processing of the slide-mounted section. The composition is a curable polymeric mixture which has high tack prior to curing, is substantially non-diffusable and non-flowable into tissue sections and, after curing, has a refractive index substantially similar to that of the specimen section and is non-labile to conventional histological solvents and stains. A preferred composition comprises diacrylate-terminated polyurethane, a diacrylate ester of an epoxy resin and a diethoxyacetophenone initiator. It is usually formed onto the mounting surface as a solution in a conventional organic solvent, such as isopropanol or toluene.Type: GrantFiled: July 30, 1993Date of Patent: August 22, 1995Assignee: Mount Sinai School of Medicine of the City University of New YorkInventor: Leonard Ornstein
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Patent number: 5427911Abstract: A process for sequencing DNA segments having complementary strands comprising (a) synthesizing simultaneously truncated products and full-length products starting from both 3' ends of the complementary strands of the DNA segment, which serves as a template, by introducing specific oligonucleotide primers annealing to the 3' ends of both complementary strands of the DNA segment, a deoxyribonucleotide elongator for each of adenine, guanine, cytosine and thymine, a thermally stable DNA polymerase and a terminator for each of adenine, guanine, cytosine and thymine, (b) thermally cycling step (a), (c) separating out the resultant truncated products and full length products according to size, and (d) selectively detecting all truncated products and full length products according to size, and (e) selectively detecting all truncated products and full-length products generated from a given strand of template.Type: GrantFiled: July 28, 1993Date of Patent: June 27, 1995Assignee: Yale UniversityInventor: Gualberto Ruano
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Patent number: 5328824Abstract: Compounds having the structure: ##STR1## wherein B represents a purine, 7-deazapurine, or pyrimidine moiety covalently bonded to the C.sup.1' -position of the sugar moiety, provided that when B is purine or 7-deazapurine, it is attached at the N.sup.9 -position of the purine or 7-deazapurine and when B is pyrimidine, it is attached at the N.sup.Type: GrantFiled: December 8, 1987Date of Patent: July 12, 1994Assignee: Yale UniversityInventors: David C. Ward, Pennina R. Langer, Alexander A. Waldrop, III
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Patent number: 5272065Abstract: Gene expression in a cell can be regulated or inhibited by incorporating into or associating with the genetic material of the cell a non-native nucleic acid sequence which is transcribed to produce an mRNA which is complementary to and capable of binding to the mRNA produced by the genetic material of said cell.Type: GrantFiled: June 21, 1990Date of Patent: December 21, 1993Assignee: Research Foundation of State University of New YorkInventors: Masayori Inouye, Takeshi Mizuno, Mei-Yin Chou
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Patent number: 5208149Abstract: Gene expression in a cell can be regulated or inhibited by incorporating into or associating with the genetic material of the cell a non-native nucleic acid sequence which is transcribed to produce an mRNA which is complementary to and capable of binding to the mRNA produced by the genetic material of said cell.Type: GrantFiled: April 10, 1992Date of Patent: May 4, 1993Assignee: The Research Foundation of State University of New YorkInventor: Masayori Inouye
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Patent number: 5190931Abstract: Gene expression in a cell can be regulated or inhibited by incorporating into or associating with the genetic material of the cell a non-native nucleic acid sequence which is transcribed to produce an mRNA which is complementary to and capable of binding to the mRNA produced by the genetic material of said cell.Type: GrantFiled: November 15, 1989Date of Patent: March 2, 1993Assignee: The Research Foundation of State University of New YorkInventor: Masayori Inouye