Abstract: Novel vectors are disclosed for expressing and secreting heterologous polypeptides from filamentous fungi. Such vectors are used in novel processes to express and secrete such heterologous polypeptides. The vectors used for transforming a filamentous fungus to express and secrete a heterologous polypeptide include a DNA sequence encoding a heterologous polypeptide and a DNA sequence encoding a signal sequence which is functional in a secretory system in a given filamentous fungus and which is operably linked to the sequence encoding the heterologous polypeptide. Such signal sequences may be the signal sequence normally associated with the heterologous polypeptides or may be derived from other sources. The vector may also contain DNA sequences encoding a promoter sequence which is functionally recognized by the filamentous fungus and which is operably linked to the DNA sequence encoding the signal sequence.

Abstract: Methods for increasing the nephron mass of a mammalian recipient are disclosed. A metanephros from an allogenic or xenogeneic mammalian donor is implanted next to a recipient's omentum or under the renal capsule of the recipient's kidney. The metanephros becomes vascularized by the recipient's blood vessels, forming a chimeric kidney that produces urine and develops a ureter that facilitates externalization of the urine. A ureter to ureter anastomosis can be subsequently performed to provide fluid communication between the chimeric kidney ureter and a ureter of the recipient.

Abstract: A container neck is used with a tamper-evident, snap-on, screw-off closure. The neck has a multiple of four first helical threads on a neck stretch portion, and at least one external ratchet tooth on a locking wall portion below the neck stretch. The closure has an upper skirt having second helical threads mating with the first helical thread of the neck and a lower skirt with at least one internal ratchet tooth shaped and positioned to engage the external ratchet tooth when the closure is applied to the neck. The teeth formed on the skirt interior and neck exterior are arranged to provide at least one circumferentially extending stretch of the tamper-evident band where the teeth are not engaged. A line of weakness extending vertically through the disengaged stretch of the tamper-evident band is ruptured when the closure is unscrewed from the neck. The number of helical threads is preferably eight and, in any event, a multiple of four.