Abstract: The present invention is directed to isolated non-coding nucleotide sequences useful as promoters for heterologous gene expression in plants. The present invention is also directed to vectors and plant cells comprising the isolated nucleotide sequences.AHAS promoters from maize are used to express introduced genes at high levels and in various plant tissues. Promoters from the als1 and als2 genes of maize are cloned and sequenced, and the promoter regions from these genes are then introduced into a plasmid 5' to the reporter gene beta-glucuronidase (GUS). Both promoter fragments are from the XI12 maize line. The als1 promoter fragment is approximately 1400 base pairs long, whereas the als2 promoter fragment contains 829 base pairs.

Abstract: An isolated polypeptide corresponding to the epitope of porcine somatotropin is provided by this invention as well as methods of enhancing the growth of an animal by administering to the animal this polypeptide in compositions.

Abstract: The present invention describes a method for inducing epizootic viral infections in insects which consists essentially of potentiating the latent virulence of indigenous insect viruses by contacting the insect with, or applying to its habitat or food supply, a potentiating amount of a stilbene compound. This invention further describes a method for protecting agronomic crops, trees, shrubs, orchards and ornamentals from attack by an insect which employs the application of a stilbene compound to a plant.

Abstract: The present invention is directed to a expression vectors and yeast cells transformed therewith containing a first heterologous nucleotide sequence which codes for a G protein-coupled receptor, for example, the somatostatin receptor, and a second nucleotide sequence which codes for all or a portion of a G protein .alpha..beta..gamma. complex. Said heterologous protein is physically expressed in a host cell membrane in proper orientation for both stereoselective binding of ligands, as well as functional interaction with G proteins on the cytoplasmic side of the cell membrane. In some embodiments, a nucleotide sequence encoding a heterologous or chimeric G.alpha. protein is expressed in conjunction with nucleotide sequences from the yeast G protein .beta..gamma. subunits. A second aspect of the present invention provides expression vectors and transformed yeast cells encoding chimeric yeast/heterologous G protein coupled receptors.

Abstract: The present invention is directed to isolated non-coding nucleotide sequences useful as promoters for heterologous gene expression in plants. The present invention is also directed to vectors and plant cells comprising the isolated nucleotide sequences.AHAS promoters from maize are used to express introduced genes at high levels and in various plant tissues. Promoters from the als1 and als2 genes of maize are cloned and sequenced, and the promoter regions from these genes are then introduced into a plasmid 5' to the reporter gene beta-glucuronidase (GUS). Both promoter fragments are from the XI12 maize line. The als1 promoter fragment is approximately 1400 base pairs long, whereas the als2 promoter fragment contains 829 base pairs.

Abstract: The present invention relates to monocot genes encoding a mutant AHAS enzyme that is specifically resistant to imidazolinone herbicides. Exemplary of these genes are corn DNA sequences which encode an amino acid substitution at position 621 of the wild-type AHAS enzyme. The mutant gene can be used to transform other plants to herbicide resistance; in this regard, the invention also provides host cells and vectors containing the gene, which cells and vectors are useful in the transformation process.

Abstract: The present invention relates to a method for isolating and cloning receptor DNA sequences. The invention also provides novel DNA sequences encoding a novel somatostatin receptor subtype.

Abstract: A first aspect of the present invention is the isolation and characterization of invertebrate (i.e. insect and nematode) potassium channel genes. The present invention provides for the isolation of complementary DNA fragments from Drosophila melanogaster and Caenorhabditis elegans which encode conserved amino acid sequence elements unique to the potassium channel gene family. A yeast expression technology is employed to clone cDNAs from Drosophila melanogaster and a hybridization approach is utilized to isolate additional cDNAs from Caenorhabditis elegans. Using the yeast expression technology, a single 2463 base pair cDNA fragment designated Dm ORF1 is isolated.

Abstract: Hybrid cell line for production of monoclonal antibody to an antigen found on normal human monocytes and granulocytes. The hybrid is formed by fusing splenocytes from immunized BALB/cJ mice with P3X63Ag8U1 myeloma cells. Diagnostic and therapeutic uses of the monoclonal antibody are also disclosed.

Abstract: A stabilized proteolytic solution has been developed which can be used to treat selected cells, especially red blood cells. A preferred stabilized solution of the invention comprises about 0.1% to about 0.3% ficin in a citrate-buffered saline solution containing mannitol, L-cysteine, dithiothrietol in an active concentration of less than about 10 mM, and EDTA. A kit for qualitatively identifying unexpected blood group antibodies comprising a series of untreated and ficin-treated red blood cells, a stabilized ficin reagent, a red blood cell diluent and an enzyme control is also provided.

Abstract: This invention relates to the use of oxidized polyamines, and especially aminoaldehydes such as spermine bisaldehyde both in vitro and in vivo to elicit an immunosuppressive response in living cells. It also relates to the therapeutic application of these compounds to induce an immunosuppressive response in living organism.

Abstract: The invention discloses nucleic acid probes specific for Neisseria gonorrhoea useful in diagnostic methods to detect the presence of N. gonorrhoea in biological specimens.

Abstract: The invention discloses nucleic acid probes specific for Neisseria gonorrhoea useful in diagnostic methods to detect the presence of N. gonorrhoea in biological specimens.

Abstract: Reagents and methods for intracellular staining of anionic materials as provided. The inherent staining characteristics of a fluorescent molecule may be suitably altered by covalently linking a plurality of said molecules to a nonimmunoglobulin derived cationic polymer having the desired staining specificity. By careful selection of the net charge and size of the dye polymer complex, diffusion through the cellular membrane can be enhanced and an enhanced fluorescent signal obtained by localization of a plurality of fluorescent molecules at the site of interest.

Abstract: The invention discloses nucleic acid probes specific for Neisseria gonorrhoea useful in diagnostic methods to detect the presence of N. gonorrhoea in biological specimens.

Abstract: A new immunoassay system is provided for the detection of ligands or ligand binding partners in solution in a heterogeneous format. The invention relies upon the detection of back scattered light from an evanescent wave disturbed by the presence of a colloidal gold label brought to the interface by an immunological reaction. The evanescent wave existing at the interface in turn is the result of a totally internally reflected incident light wave. Placement of the detector at a back angle above the critical angle insures a superior signal-to-noise ratio.

Abstract: An assay device is provided which includes a support, generally planar and elongated in shape, upon which a biological binding reaction may take place. This assay support includes in its broadest aspect, three sections, a sample receiving well section at one end and a gripping section at the opposite end, with a connecting section therebetween. The sample receiving well section includes at least two spaced-apart receiving wells, generally at the end of the assay support, for the addition of binding reactants. Each of the wells has an open end on a first face of the support, a concave inner surface extending concavely into the support, and a convex outer surface which projects convexly from an opposed face of the support. These wells may serve as a depository to contain aliquots of binding reagents, as well as a sample under analysis, the sample under analysis possibly containing a substance that will bind to the binding reagents.

Abstract: A new immunoassay system is provided for the detection of ligands or ligand binding partners in solution in a heterogeneous format. The invention relies upon the detection of back scattered light from an evanescent wave disturbed by the presence of a colloidal gold label brought to the interface by an immunological reaction. The evanescent wave existing at the interface in turn is the result of a totally internally reflected incident light wave. Placement of the detector at a back angle above the critical angle insures a superior signal-to-noise ratio. Apparatus and methods for scanning, detecting and manipulating light including a scattered total internal reflectance immunoassay system are provided.

Abstract: Hybrid cell line for production of monoclonal antibody to an antigen found on normal human monocytes and granulocytes. The hybrid is formed by fusing splenocytes from immunized BALB/cJ mice with P3X63Ag8Ul myeloma cells. Diagnostic and therapeutic uses of the monoclonal antibody are also disclosed.

Abstract: Hybrid cell line for production of monoclonal antibody to an antigen found on approximately 95% of normal human thymocytes. The hybrid is formed by fusing splenocytes from immunized CAF.sub.1 mice with P3X63Ag8U1 myeloma cells. Diagnostic and therapeutic uses of the monoclonal antibody are also disclosed.