Patents Represented by Attorney, Agent or Law Firm Genencor International
  • Production of ascorbic acid
    Patent number: 6824996
    Abstract: The present invention provides for the production of ASA from yeast capable of producing ASA from KLG. The present invention provides methods for the production of ASA as well as recombinant yeast capable of producing ASA from a carbon source.
    Type: Grant
    Filed: December 18, 2001
    Date of Patent: November 30, 2004
    Assignee: Genencor International, Inc.
    Inventor: Manoj Kumar
  • Multiply-substituted protease variants
    Patent number: 6815193
    Abstract: Novel protease variants derived from the DNA sequences of naturally-occurring or recombinant non-human proteases are disclosed. The variant proteases, in general, are obtained by in vitro modification of a precursor DNA sequence encoding the naturally-occurring or recombinant protease to generate the substitution of a plurality of amino acid residues in the amino acid sequence of a precursor protease. Such variant proteases have properties which are different from those of the precursor protease, such as altered wash performance.
    Type: Grant
    Filed: November 2, 2001
    Date of Patent: November 9, 2004
    Assignee: Genencor International, Inc.
    Inventors: Ayrookaran J. Poulose, Volker Schellenberger, James T. Kellis, Jr., Christian Paech, Joanne Nadherny, Donald P. Naki, Katherine D. Collier, Robert M. Caldwell, André C. Baeck
  • Production of ascorbic acid
    Patent number: 6808918
    Abstract: The present invention provides for the production of ASA from yeast capable of producing ASA from KLG. The present invention provides methods for the production of ASA as well as recombinant yeast capable of producing ASA from a carbon source.
    Type: Grant
    Filed: December 18, 2001
    Date of Patent: October 26, 2004
    Assignee: Genencor International, Inc.
    Inventor: Manoj Kumar
  • Proteases from gram positive organisms
    Patent number: 6794179
    Abstract: The present invention relates to the identification of a novel metalloprotease in gram positive microorganisms. The present invention provides the nucleic acid and amino acid sequences for the metalloprotease. The present invention also provides host cells having a mutation or deletion of part or all of the gene encoding the metalloprotease. The present invention provides host cells which further comprises a nucleic acid encoding desired heterologous proteins such as enzymes. The present invention also provides cleaning compositions, animal feeds and compositions used to treat a textile that include the metalloprotease of the present invention.
    Type: Grant
    Filed: July 2, 2002
    Date of Patent: September 21, 2004
    Assignee: Genencor International, Inc.
    Inventor: David A. Estell
  • Granule containing enzyme, corn starch and sugar layered on an inert particle
    Patent number: 6790643
    Abstract: Granules that include a protein core are described. The protein core includes a protein matrix which includes a protein mixed together with a starch and optionally sugar such as sucrose. The protein matrix can be layered over a seed particle or the protein core can be homogeneous. The protein can be an enzyme or a therapeutic protein. A barrier layer may surround the protein core, and a coating can be applied to the seed particle, the protein matrix and/or the barrier layer.
    Type: Grant
    Filed: June 25, 2002
    Date of Patent: September 14, 2004
    Assignee: Genencor International, Inc.
    Inventors: Nathaniel T. Becker, Thomas S. Green
  • Xylanase from trichoderma reesei, method for production thereof, and methods employing this enzyme
    Patent number: 6768001
    Abstract: The present invention is directed to novel xylanases (referred to as XYL-IV) and to nucleic acid molecules encoding those xylanases. Also provided herein are vectors and host cells including those nucleic acid sequences, antibodies which bind to the xylanases of the present invention, methods for producing the xylanases of the present invention, and methods employing the xylanases of the present invention.
    Type: Grant
    Filed: May 31, 2002
    Date of Patent: July 27, 2004
    Assignee: Genencor International, Inc.
    Inventors: Markku L A Saloheimo, Matti Siika-Aho, Maija Tenkanen, Merja E. Penttila
  • Bacillus subtillis with an inactivated cysteine protease-1
    Patent number: 6762039
    Abstract: The present invention relates to the identification of novel cysteine proteases in Gram-positive microorganisms. The present invention provides the nucleic acid and amino acid sequences for the Bacillus subtilis cysteine proteases CP1, CP2 and CP3. The present invention also provides host cells having a mutation or deletion of part or all of the gene encoding CP1, CP2 or CP3. The present invention also provides host cells further comprising nucleic acid encoding desired heterologous proteins such as enzymes. The present invention also provides a cleaning composition comprising a cysteine protease of the present invention.
    Type: Grant
    Filed: January 13, 2000
    Date of Patent: July 13, 2004
    Assignee: Genencor International, Inc.
    Inventor: David A. Estell
  • Proteases from gram-positive organisms
    Patent number: 6723550
    Abstract: The present invention relates to the identification of novel serine proteases in Gram-positive microorganisms. The present invention provides the nucleic acid and amino acid sequences for the Bacillus subtilis serine proteases SP1, SP2, SP3, SP4 and SP5. The present invention also provides host cells having a mutation or deletion of part or all of the gene encoding SP1, SP2, SP3, SP4 and SP5. The present invention also provides host cells further comprising nucleic acid encoding desired heterologous proteins such as enzymes. The present invention also provides a cleaning composition comprising a serine protease of the present invention.
    Type: Grant
    Filed: January 13, 2000
    Date of Patent: April 20, 2004
    Assignee: Genencor International, Inc.
    Inventor: David A. Estell
  • Directed evolution of microorganisms
    Patent number: 6706503
    Abstract: The present invention provides methods for directing the evolution of microorganisms comprising the use of mutator genes and growth under conditions of selective pressure. The method discloses mutator genes which can be used in the methods of the present invention and provides ATCC deposits which exemplify the evolved microorganisms produced by the methods.
    Type: Grant
    Filed: October 23, 2001
    Date of Patent: March 16, 2004
    Assignee: Genencor International, Inc.
    Inventors: Volker Schellenberger, Amy D. Liu, Olga V. Selifonova
  • Secretion factors for gram-positive micoorganisms, genes encoding them and methods of using it
    Patent number: 6692951
    Abstract: Heterologous protein secretion from Gram-positive bacteria, in particular from Bacilli has, with few exceptions, met with little success. Incompatibility of the heterologous proteins with the protein secretion machinery of the host is the main cause of this effect. This limiting factor for the production of heterologous proteins in commercially significant concentrations from Bacillus subtilis is removed by overexpressing the Bacillus subtilis protein FtsY or FtsY protein in combination with overexpression of other members of the bacterial signal recognition particle. Said gene(s) is(are) overexpressed in Bacillus host cells expressing a heterologous protein which then shows an increased amount of the heterologous protein secreted in the surrounding medium.
    Type: Grant
    Filed: May 17, 2002
    Date of Patent: February 17, 2004
    Assignee: Genencor International, Inc.
    Inventors: Wilhelmus J. Quax, Richard Kerkman, Cornelis P. Broekhuizen
  • Enzymatic bleaching of natural non-cotton cellulosic fibers
    Patent number: 6685748
    Abstract: A method is provided for bleaching a non-cotton cellulosic fiber, yarn or fabric by contacting said fiber, yarn or fabric with a hemicellulase enzyme for a time and under conditions suitable to produce a whitening of said fiber, yarn or fabric.
    Type: Grant
    Filed: April 4, 2000
    Date of Patent: February 3, 2004
    Assignee: Genencor International, Inc.
    Inventors: Anthony G. Day, Scott D. Power, Doreen Victoria, Deborah S. Winetzky
  • Accelerated stability test
    Patent number: 6653093
    Abstract: Enzyme granulates contain besides the enzyme water-soluble and water-insoluble fillers, binders and if required other additives for granulates. In order to stabilize the enzyme, an alkaline or earth alkaline metal formiate is added, as well as reducing sugar, if required. These enzyme granulates may contain all usual enzymes for washing and cleaning purposes, in particular alkaline proteases. A process for preparing these enzyme granulates, as well as their use in powdery washing product compositions are also disclosed.
    Type: Grant
    Filed: June 13, 2000
    Date of Patent: November 25, 2003
    Assignee: Genencor International, Inc.
    Inventors: Peter F. Plank, Thomas P Graycar, Matthew J. Flynn
  • Human protease and use of such protease for pharmaceutical applications and for reducing the allergenicity of non-human proteins
    Patent number: 6642011
    Abstract: The present invention relates to a method of producing novel improved protein mutant which produce low allergenic response in humans compared to the parent of that mutant. Specifically, the present invention comprises neutralizing or reducing the allergenicity of a protein by introducing therein as replacement or modification of an epitope on such protein a sequence from human subtilisin.
    Type: Grant
    Filed: April 15, 1998
    Date of Patent: November 4, 2003
    Assignee: Genencor International, Inc.
    Inventor: David A. Estell
  • Carbon dioxide soluble surfactant having two fluoroether CO2-philic tail groups and a head group
    Patent number: 6638749
    Abstract: There is provided a process for the extraction of water soluble biomaterials such as enzymes or proteins into carbon dioxide utilizing certain carbon dioxide-soluble surfactants. Also provided are certain carbon dioxide-soluble surfactants useful in the extraction of proteins. The surfactants are selected from fluoroether sulfate, fluoroether-polyethylene glycol block copolymer, fluoroether-functional sorbitol, and fluoroether dithiocarbamate chelate.
    Type: Grant
    Filed: January 8, 1998
    Date of Patent: October 28, 2003
    Assignees: Genencor International, Inc., University of Pittsburgh
    Inventors: Eric J. Beckman, Eliador J. Ghenciu, Nathaniel T. Becker, Landon M. Steele, Alan J. Russell
  • Fluidized bed low density granule
    Patent number: 6635611
    Abstract: Low-density enzyme-carrying granules are low dusting and/or storage-stable, and especially suitable for use in liquid detergents and cleaners, such as non-aqueous liquid laundry detergents. Preferred granules of the invention include a relatively high content of one or more low-density fillers, such as perlite or starch, to provide a desired product density. In one embodiment, the granules have a true density within a range of from about 1 to about 1.4 g/cm3. The granules can be economically produced in commercial quantities using fluidized bed technology.
    Type: Grant
    Filed: May 25, 2001
    Date of Patent: October 21, 2003
    Assignee: Genencor International, Inc.
    Inventor: Douglas A. Dale
  • Mutant EGIII cellulase, DNA encoding such EGIII compositions and methods for obtaining same
    Patent number: 6635465
    Abstract: The present invention relates to variant EGIII cellulases that have improved stability and/or performance. The variant cellulases have replacements at sensitive residues to improve stability and/or performance.
    Type: Grant
    Filed: August 4, 2000
    Date of Patent: October 21, 2003
    Assignee: Genencor International, Inc.
    Inventors: Peter Gualfetti, Colin Mitchinson, Traci H. Ropp
  • Increasing production of proteins in gram-positive microorganisms
    Patent number: 6630328
    Abstract: The present invention relates to secretion in Gram-positive microorganisms. The present invention provides the nucleic acid amino acid sequences for the Bacillus subtilis secretion factors SecDF. The present invention also provides improved methods for the secretion of heterologous or homologous proteins in gram-positive microorganisms.
    Type: Grant
    Filed: July 5, 2001
    Date of Patent: October 7, 2003
    Assignee: Genencor International, Inc.
    Inventor: Wilhelmus J. Quax
  • Variant EGIII-like cellulase compositions
    Patent number: 6623949
    Abstract: The present invention relates to novel variant EGIII or EGIII-like cellulases that have improved stability. The variant cellulases have performance sensitive residues replaced to a residue having modified stability.
    Type: Grant
    Filed: August 4, 2000
    Date of Patent: September 23, 2003
    Assignee: Genencor International, Inc.
    Inventors: Peter Gualfetti, Colin Mitchinson, Jay Phillips
  • Method and compositions for treating cellulose containing fabrics using truncated cellulase enzyme compositions
    Patent number: 6620605
    Abstract: Improved methods of treating cellulose containing fabrics with cellulase comprising contacting the cellulose fabrics with truncated cellulase enzyme. Treatment of cellulose containing fabrics with cellulase core domains of the invention are disclosed as offering specific advantages of reduced redeposition of dye and increased abrasion.
    Type: Grant
    Filed: July 27, 2001
    Date of Patent: September 16, 2003
    Assignee: Genencor International, Inc.
    Inventors: Timothy Fowler, Kathleen A. Clarkson, Michael Ward, Katherine D. Collier, Edmund Larenas
  • Cleaning compositions containing plant cell wall degrading enzymes and their use in cleaning methods
    Patent number: 6602842
    Abstract: Novel cleaning compositions comprising cell wall degrading enzymes are disclosed having pectinases and/or hemicellulases and optionally cellulases. The compositions are particularly suitable for removing stains of vegetable origin, especially from textiles. Although compositions having only one type of such enzymes axe part of the invention (excluding cellulases alone), preferred embodiments have a mixture of cell wall degrading enzyme activities to allow for a concerted action against the fibrous mass which usually constitutes a stain of vegetable origin.
    Type: Grant
    Filed: April 5, 2001
    Date of Patent: August 5, 2003
    Assignee: Genencor International, Inc.
    Inventors: Roelck A. Cuperus, Margareta A. Herweijer, Albert J. J. Van Ooijen, Dick J. Van Schouwen