Abstract: A method of purifying granulocyte-macrophage colony-stimulating factor (GM-CSF), particularly recombinant human GM-CSF, in good yield and with retention of biological activity, comprising sequential anion-exchange, dye-ligand affinity, gel filtration and reversed-phase chromatography is disclosed.
Type:
Grant
Filed:
September 21, 1993
Date of Patent:
February 21, 1995
Assignee:
Schering Plough Corporation
Inventors:
Paul P. Trotta, Gail F. Seelig, Robert A. Kosecki, Paul Reichert