Abstract: Disclosed are pH dependent ion exchange matrices, methods of making, and methods of use.

Abstract: A method is provided for using a silanized silica matrix to isolate a target nucleic acids, such as plasmid DNA, fragments of DNA, chromosomal DNA, or RNA from contaminants, including proteins, lipids, cellular debris, or non-target nucleic acids. The silanized silica matrix comprises a silica based solid phase and a plurality of silane ligands covalently attached to the surface of the solid phase. Non-target material adsorbs to the silanized silica matrix in the presence of a sufficient concentration of chaotropic salt, while target nucleic acids adsorb to the matrix under other solution conditions. The method of using the silanized silica matrix of the present invention can be used to clear solutions of disrupted biological material, and to isolate nucleic acids therefrom or from other solutions containing nucleic acids and at least one contaminant.

Abstract: The present invention is directed to materials and methods for the identification and analysis of intermediate tandem repeat sequences in DNA, wherein an intermediate tandem repeat (ITR) sequence is a region of a DNA sequence containing at least one five to seven base repeat unit appearing in tandem at least two times. DNA markers to highly polymorphic ITR loci in the human genome are identified and analyzed, using particularly preferred embodiments of the materials and methods of the present invention.

Abstract: A process for producing and purifying peptides and for producing peptide/protein antigens for antibody production is described. The process utilizes fusion proteins and specifically fusion proteins in which the fusion protein carrier segment includes an amino acid sequence at least about 65 amino acids long and in which the amino acid sequence does not contain negative or positive charged side chains of amino acids.

Abstract: A compound of formula (I), wherein: R2 and R3 are independently selected from H, R, OH, OR, ═O, ═CH—R, ═CH2, CH2—CO2R, CH2—CO2H, CH2—SO2R, O—SO2R, CO2R, COR and CN, and there is optionally a double bond between C1 and C2 or C2 and C3; R6, R7, R8 and R9 are independently selected from H, R, OH, OR, halo, nitro, amino, Me3Sn; R11 is either H or R; Q is S, O or NH; L is a linking group, or a single bond; O is a solid support; or where one or more of R2, R3, R6, R7 and R8 are independently: H—(T)n—X—Y—A— where: X is CO, NH, S or O; T is a combinatorial unit; Y is a divalent group such that HY=R; A is O, S, NH, or a single bond and n is a positive integer

Abstract: This invention is a prosthetic device generally placed on the outside surface of the vessel or graft which then elutes antiproliferative drugs or agents from a drug-eluting matrix material. Methods of perivascular antiproliferative drug administration also are disclosed.

Abstract: An apparatus for the electrically assisted delivery of therapeutic agent is described. An apparatus of the invention has rigid zones or regions which are physically connected by flexible means such as a web. The flexible means permits the rigid zones to move independently with respect to each other while remaining physically connected or coupled. In a preferred embodiment, the rigid zones are physically and electronically coupled by the flexible means. In another preferred embodiment, the skin side of the rigid zones has a radius of curvature which approximates that of the body site to which the device is to be attached. A method of increasing the body or surface conformability of a rigid electrotransport device is described. The method involves the step of intentionally placing rigid subcomponents of the device in physically separate zones within the device. The rigid zones are separate and are coupled by flexible connector means.

Abstract: A remote two-module electronic fluid monitoring system to be used as an agricultural aid in the application of a fluid to a field. A tank module sits atop a field tank and monitors internal fluid level. Fluid level detection is achieved by tracking the position of an embedded permanent magnet associated with a given internal fluid level within an existing float gauge mechanism. An integrated circuit capable of precisely detecting the orientation of magnetic fields senses the angular position of an existing magnet and outputs an angular field reading to an interfaced microcontroller which then translates angular reading to fluid level. The tank module contains a radio frequency transmitter which then sends fluid level information to a display module mounted within the tractor cab. The display module receives the signal from the tank module and reports to the user the present fluid level remaining in tank via a liquid crystal display.

Abstract: A butterfly sponge mop (1) which has a mop handle (2) which can be set at a desired angle with respect to the wings (12,14) of the mop. The handle is attached to a swivel housing (34) which is provided between the wings, by a swivel connector (32) provided with a swivel pin (38). The axis of the swivel pin is at an angle to the axis of the handle so that rotation of the handle and swivel connector sets the angle of the handle.

Abstract: A toilet brush having a frame member (1), a handle portion (3) and a brush portion (5). The brush portion includes front bristles (13) for cleaning a toilet bowl and rearwardly extending bristles (15) for cleaning the lip of a toilet. The bristles and a soft covering (19) of the handle have been moulded onto the frame member using a soft rubbery plastics composition.

Abstract: There is provided an apparatus (LCPA) for measuring a load applied to a quantity of material. The LCPA includes a load cell assembly sandwiched between an inner plate and an outer plate. The load cell assembly measures the load applied to the quantity of material when a force is exerted against the outer plate. Preferably, the load cell assembly includes three equally spaced apart load cells. In one aspect, the LCPA is a part of a gyratory compactor such that the LCPA measures the load applied to a paving material test specimen when a ram exerts a force against the outer plate during the compaction and gyration process. In one aspect, the location of the resultant force exerted by the ram on the specimen is calculated so that the performance of the test specimen may be evaluated.

Abstract: A hollow lumen cable is formed by helically winding inner and outer coils with the helices of each coil being in an abutting relationship and the outer coil inner peripheral diameter being less than the outer peripheral diameter of the inner coil. Preferably, each coil is multifilar. After the coils are wound, an unwinding force is applied to the outer coil and the inner coil is inserted into the outer coil to have its winding inclined opposite to that of the outer coil; and thence, the outer coil is allowed to contract to form an interference fit with the inner coil. The cable may be incorporated as part of a medical apparatus. The cable is bendable about the tight radii and has a high torque transfer resolution while having a small cable outer diameter, a relatively long life, and has a hollow lumen in which there is no structure or through which fluid, wires or medial devices may pass.

Abstract: The present invention provides novel carbamate-based cationic lipids of the general structure: or a salt, or solvate, or enantiomers thereof, wherein (a) R1 is a lipophilic moiety; (b) R2 is a positively charged moiety; (c) n is an integer from 1 to 8; (d) X is an anion or polyanion; and (e) m is an integer from 0 to a number equivalent to the positive charge(s) present on the lipid. The present invention further provides compositions of these lipids with polyanionic macromolecules, methods for interfering with protein expression in a cell utilizing these compositions, and a kit for preparing the same.

Abstract: Derivatives of isoquinolinone and dihydrolisoquinolinone, and related compounds, and their use as pharmaceuticals in the treatment of a disease by inhibition of the enzyme poly(ADP-ribose)polymerase (“PARP”) are disclosed.

Abstract: The present invention provides novel amide-based cationic lipids of the general structure: or a salt, or solvate, or enantiomers thereof wherein; (a) Y is a direct link or an alkylene of 1 to about 20 carbon atoms; (b) R1 is H or a lipophilic moiety; (c) R2, R3, and R4 are positively charged moieties, or at least one but not all of R2, R3, or R4 is a positive moiety and the remaining are independently selected from H, an alkyl moiety of 1 to about 6 carbon atoms, or a heterocyclic moiety of about 5 to about 10 carbon atoms; (d) n and p are independently selected integers from 0 to 8, such that the sum of n and o is from 1 to 16; (e) X− is an anion or polyanion and (f) m is an integer from 0 to a number equivalent to the positive charge(s) present on the lipid; provided that if Y is a direct link and the sum of n and p is 1 then one of either R3 or R4 must have an alkyl moiety of at least 10 carbon atoms.

Abstract: A high refractive index, foldable polymer suitable for use in ophthalmic devices, such as intraocular lenses, is provided. The polymer may be produced from a polymerization reaction of first, second and third monomeric components and a crosslinking agent. The first monomeric component includes an aryl acrylate or an aryl methacrylate. The second monomeric component, which is not an acrylate, includes a monomer having an aromatic ring with a substituent having at least one site of ethylenic unsaturation. The third monomeric component includes a high water content hydrogel-forming monomer. The resulting high refractive index copolymer is durable enough to be cut and polished when dry, and becomes soft and foldable when hydrated.

Abstract: A method for producing paper pulp for use in the making of paper from eucalyptus wood chips. The method comprises inoculating wood chips with white rot fungi, fermenting the wood chips so as to cause a propagation of the fungus through the wood chips and allowing the fungus to modify the lignin, and pulping the degraded wood chips by a kraft process.

Abstract: Disclosed are alkyl peptide amides capable of inhibiting or reducing sperm cell motility. Methods of inhibiting or reducing sperm motility using certain alkyl peptide amides are also disclosed.

Abstract: A compound of formula (IV): O is a solid support; L is a linking group or a single bond; X′ is selected from CO, NH, S, or O; A is O, S, NH, or a single bond; R2 and R3 are independently selected from: H, R, OH, OR, ═O, ═CH—R, ═CH2, CH2—CO2R′, CH2—CO2H, CH2—SO2R, O—SO2R, CO2R, COR, CN and there is optionally a double bond between C1 and C2 or C2 and C3; R6, R7, and R9 are independently selected from H, R, OH, OR, halo, nitro, amino, Me3Sn; R11 is either H or R; Q is S, O or NH; R10 is a nitrogen protecting group; and Y is a divalent group such that HY=R, and other related compounds and collections of compounds.

Abstract: A compound with formula (I) where R10 is a therapeutically removable nitrogen protecting group; R2 and R3 are independently selected from: H, R, OH, OR, ═O, ═CH—R, ═CH2, CH2—CO2R, CH2—CO2H, CH2—SO2R, O—SO2—R, CO2R, COR and CN; R6, R7 and R9 are independently selected from H, R, OH, OR, halo, amino, nitro, Me3Sn; X is S, O or NH; R11 is either H or R; and where there is optionally a double bond between C1 and C2 or C2 and C3; and R8 is selected from H, R, OH, OR, halo, amino, nitro, Me3Sn, or R7 and R8 together form a group —O—(CH2)p—O—, where p is 1 or 2. Such compounds may be used in methods of ADEPT, GDEPT, NPEPT or PDT.