Abstract: Recombinant DNA polymerases from archaebacteria as well as isolated DNA coding for such polymerases are provided. The isolated DNA is obtained by use or DNA or antibody probes prepared from the DNA encoding T. litoralis DNA polymerase and the T. litoralis DNA polymerase respectively. Also provided are methods for producing recombinant archaebacteria thermostable DNA polymerase and methods for enhancing the expression of such polymerases by identifying, locating and removing introns from within the DNA coding for such DNA polymerases.
Type:
Grant
Filed:
April 4, 1994
Date of Patent:
November 10, 1998
Assignee:
New England Biolabs, Inc.
Inventors:
Donald G. Comb, Francine Perler, Rebecca Kucera, William E. Jack