Abstract: The addition of buffer to a harvest stream during diafiltration can cause increased turbidity and have other undesirable effects including limiting product recovery. Methods and compositions related to the use of a non-ionic surfactant are provided for improving diafiltration.

Abstract: Separation methods, for example, to isolate a recombinant protein, are disclosed. In some implementations, a method includes forming a solid containing a first cation and a first anion in a medium containing a protein, and separating the solid from the protein.

Abstract: A retractable roof closure for a vehicle body having a windshield header spaced from a fixed rear roof panel to provide an unobstructed roof opening. The closure panel has its rearward end supported and guided by the cooperation of a guide track extending along each side of the vehicle body beneath the fixed roof panel and guide rollers mounted on the rear end of the closure panel and engaged within the guide track. The forward end of the closure panel is supported by a telescopable extension device on each end of the vehicle body. Each telescopable extension device includes a first member mounted along the fixed roof panel and a second member having its forward end pivotally connected to the forward end of the panel.

Abstract: A buckle component of a vehicle occupant restraint belt system is slidably disposed on a restraint belt of the system in an improved manner. The buckle component may take the form of a female buckle or a male D-ring that secures the belt in an occupant restraining position. The buckle component includes a housing with an outwardly facing rectilinear locking portion extending transversely with respect to the elongated direction of the belt. An external lock bar extends alongside the locking portion with the belt received therebetween. The lock bar has a slightly greater length than the width of the belt and has end portions extending about the side edges of the belt and back toward each other in generally U-shaped configurations. These end portions are pivotally mounted on the housing of the buckle component.

Abstract: A sliding vehicle door has a guard beam therein having flanges at its upper and lower edges. An extruded carriage member is associated with each of the flanges and slidably engages the flange to permit longitudinal movement of the guard beam and the door on the carriage member. A hinge arm is associated with each of the extruded carriage members and has one end pivoted to the carriage member and the other end pivoted to a body pillar defining the door opening. An articulating link acts between the body pillar and one of the carriage members to maintain the door in substantial parallel relationship with the vehicle longitudinal axis during pivotal movement of the hinge arms to move the door laterally of the body between a closed position in the plane of the door opening and an open position laterally outboard of the door opening.