Abstract: A method for extraction of cholesterol from untreated yolk with supercritical carbon dioxide. The supercritical carbon dioxide extraction of cholesterol from the liquid egg yolk is performed in the presence or absence of cosolvents or other agents enhancing the cholesterol extraction. All agents are non-toxic and safe for human consumption.

Abstract: Transplants are coated with an insoluble immunological barrier membrane. The membrane can comprise a non-cytotoxic first layer of agarose and cationic amino acid polymer and a non-cytotoxic second layer chemically bonded to said first layer, the second layer comprising an anionic amino acid polymer. The molecular weight of the anionic amino acid polymer is preferably in the range of 2000 to 500,000 daltons, and the thickness if the total membrane coating is within the range of 1 and 100 microns. Alternatively, the membrane can comprise a non-cytotoxic first layer of a cationic amino acid polymer. A non-cytotoxic second layer chemically bonded to the first layer comprises agarose and an anionic amino acid polymer. A non-cytotoxic optional third layer chemically bonded to said second layer comprises a cationic amino acid polymer.

Abstract: A method for extraction and removal of cholesterol from untreated liquid egg yolk with simultaneous production of cholesterol as a by-product. The removal of cholesterol from the untreated liquid egg yolk is achieved by the extraction of cholesterol into a vegetable oil by ultra high pressure homogenization producing liquid egg yolk having reduced amount of cholesterol. The cholesterol extracted into the vegetable oil is further removed from the oil by steam stripping method, allowing a recycling of the steam stripped extraction oil. Cholesterol is recovered as the extraction by-product from the aqueous steam stripping distillate.

Abstract: A high temperature furnace for heat treatment of various products, and for sintering. One of the uses is for preparing dental products such as dental crowns, bridges, implants and the like. The main component of the furnace is a cup-shaped quartz vessel, with a curved roof section, and which has a wide flange at its open end. There is provided a heating coil, applied to the vessel, and heat insulating means and heat measuring means indicating the temperature inside the vessel. There is provided a tube leading to the outside which can be used for establishing a vacuum in the vessel or for providing in the vessel or for providing in the vessel an atmosphere of any desired gas or gas mixture. There is provided a base through which there can be raised and lowered a worktable. The flange of the vessel rests on O-rings which provide a hermetic seal, and which are not subjected to an excessive temperature due to the width to the width of the flange of the vessel.

Abstract: A transplant comprising a core of a viable, physiologically active cells coated with a non-fibrogenic alkaline earth metal alginate free from fibrogenic amounts of fucose, sulfate, phloroglucinol and protein moieties, having a mannuronate to guluronate molar ratio of from 1.2 to 6. The coating protects the core from host immunological destruction after transplantation. The coating is sufficiently permeable to permit the diffusion of nutrients and cell products through the coating.A process for coating the transplant core comprising coating the core with alginate substantially free from fibrogenic compounds and reacting the alginate coating with alkaline earth metal cations comprising calcium ions or magnesium ions or mixture thereof to form an alkaline earth metal alginate coating.

Abstract: A method is described for the use of monoclonal antibodies in a sensitive immunoassay for halogenated dioxins and dibenzofurans in industrial samples which contain impurities. Appropriate sample preparation and selective enzyme amplification of the immunoassay sensitivity permits detection of dioxin contaminants in industrial or environmental samples at concentrations in the range of a few parts per trillion.

Abstract: A device and method for establishing and maintaining a gas discharge extending essentially through the entire space between electrodes which face each other. The gas discharge is obtained by providing on at least one of the surfaces of the electrodes facing each other a thin dielectric coating, and applying a suitable current, in the range of from about 50 KHz to about 2 MHz. A high frequency excitation voltage is used, and the thin dielectric coating acts as a capacitor.

Abstract: A highly specific and sensitive electrode for the determination of ultra-low levels of mercury and an analytical system based on such electrode. The electrode is a glassy carbon electrode spin-coated with a monolayer of a highly sensitive reagent for the detection of mercury. The analytical method based on the use of this type of electrode is a voltammetric method. Concentrations of the order of as low as about 2.10.sup.-12 Moles mercury can be detected and measured. The reagent is 4,7,13,16,21,24-hexaoxa-1,10-diazabicyclo-[8.8.8]hexacosane.

Abstract: A process is provided for the preparation of 10-deazaaminopterins and 5,10- and 8,10-dideazaaminopterins starting from the corresponding homoterephthalic acid diester coupling the corresponding dicarboxylic acid diester having the formula: ##STR1## with the corresponding diaminopterin 6-methylene halide having the formula: ##STR2## thereby forming a pteroic acid diester having the formula: ##STR3## hydrolysing the two ester groups to form the corresponding carboxylic acid groups, and then monodecarboxylating the diacid, thereby removing the carboxylic acid group attached to the carbon alpha to the A group and forming a pteroic acid.

Abstract: A method of preventing clinically manifest Pneumocystis carinii pneumonia in immunocompromised humans is provided wherein an aerosol of pentamidine is administered to the patient in a manner which delivers the aerosol to the patient's alveoli. Also provided is a method of treating Pneumocystis carinii pneumonia in HIV seropositive individuals comprising administering a therapeutically effective amount of pentamidine aerosol to the HIV positive individual through inhalation. In addition, a method is provided of administering pentamidine to a human comprising aerosolizing a pentamidine solution in a nebulizer system having a one-way valve disposed between the nebulizer and the mouthpiece of the nebulizer system.

Abstract: A method of preventing clinically manifest Pneumocystis carinii pneumonia in immunocompromised humans is provided wherein an aerosol of pentamidine is administered to the patient in a manner which delivers the aerosol to the patient's alveoli. Also provided is a method of treating Pneumocystis carinii pneumonia in HIV seropositive individuals comprising administering a therapeutically effective amount of pentamidine aerosol to the HIV positive individual through inhalation. In addition, a method is provided of administering pentamidine to a human comprising aerosolizing a pentamidine solution in a nebulizer system having a one-way valve disposed between the nebulizer and the mouthpiece of the nebulizer system.

Abstract: There is disclosed certain heteroaroyl 10-deazaaminopterin and 5, 10 and 8, 10 di deazaminopterin compounds and their use for treatment of rheumatoid arthritis and related diseases and preparative process.Also disclosed are 10 alkenyl-(and alkynyl) 10-deazaminopterins also disclosed for treatment of rheumatoid arthritis and for leukemia and ascites tumors and preparative process.

Abstract: Methods are described for making specific monoclonal antibodies useful for detection of cyclodienes in foods and environmental samples. Monoclonal antibodies specifically reactive with cyclodienes can detect accumulated pesticides in food, tissue or environmental samples. Extraction and preparation of organic samples for immunoassay in a polar-nonpolar reaction medium permits detection of halogenated organic ring structures at concentrations in samples.

Abstract: Novel photosensitizers useful for photodynamic therapy and diagnosis of tumors. Photosensitizers are derived from chlorophyll-a of photosynthetic plants and algae, possess long wavelength absorption between 600 and 800 nm, are stable, and water soluble. A process of preparation of these compounds and method of use for diagnostic and therapeutic purposes.

Abstract: A novel recombinant streptavidin-protein A chimeric protein which allows conjugation of antibody molecules with biological materials. The chimeric protein is efficiently expressed in Escherichia coli and is purified by simple procedures. The purified chimetic protein can bind one biotin molecule and one to two immunoglobulin molecules per subunit.

Abstract: Compounds and a method useful for protection of tissue cells in a mammalian body from irreversible damages due to lactic acidosis caused by oxygen deficiency. The protection is achieved by administering a compound having a cell membrane permeability and/or ability to cross the blood brain barrier and being able to provide a buffering action to prevent an increase in a hydrogen ion concentration over the physiologically acceptable levels.

Abstract: A method and assay for determination of functionality of steroid receptors present in breast or other tumors. The method is useful for determination of cancer responsiveness to hormonal therapy by establishing a correlation between functioning estrogen receptors and those which are dysfunctional or nonfunctional. The assay is useful for determination whether the cancer, particularly the breast cancer, will respond to anti-estrogen hormonal therapy.

Abstract: The use of the active ingredient present in the juice obtained from the green hull of the kukui nut for use as an antiviral pharmaceutical agent. This active ingredient is particularly useful in the topical treatment of viral infections, such as herpes simplex, genital herpes, genital warts, herpes zoster and chickenpox. The raw liquid juice, or the juice formulated in a pharmaceutically acceptable carrier, is topically administered to the surface lesion caused by the virus. Some components present of the active ingredient in the kukui nut hull juice include methyl esters of long chain fatty acids and their isomers.

Abstract: A method for long range transcontinental or transoceanic transport of fresh chilled meat. The method concerns a transport of vacuum packaged chilled beef, pork or lamb meat or poultry or fish in refrigerated containers wherein one part of the container is filled with the frozen meat and the fresh non-frozen but chilled meat is placed in the close proximity of the frozen meat and insulated with foamed polystyrene laminated on both sides with sheets of insulated material. The chilled meat is stored in controlled atmosphere containing less then 1% of oxygen.

Abstract: An iontophoretic bioelectrode includes an enclosure forming upper sheet of material having a generally planar outer section circumscribing a raised central section which defines an interior compartment. A compliant, resilient and sticky raised barrier is formed on the underside of the outer section of the upper sheet of material to circumscribe the central section. When placed against the skin of a person, the barrier adheres to the skin to form a seal completely around the central section and interior compartment into which an ion carrying solution may then be introduced for administration, by iontophoresis, into a persons' skin.