Abstract: The abstract discloses a method for preparing supports for the assay of chemical or biological substances, consisting in preparing N different supports, each being homogeneous for a type of reaction, then in mutually combining, according to a predetermined arrangement, all or part of the said homogenous N supports to constitute assay supports having each an ordered set of reaction zones of N different types or less. The invention also concerns the assay supports prepared by this method and their use in processes of immunological hybridisation assays.