Abstract: This invention provides: novel protein homologous of a Kunitz domain, which are capable of binding kallikrein; polynucleotides that encode such novel proteins; and vectors and transformed host cells containing these polynucleotides.
Type:
Grant
Filed:
October 19, 1999
Date of Patent:
December 25, 2001
Assignee:
Dyax Corp.
Inventors:
William Markland, Robert Charles Ladner
Abstract: A method is disclosed for obtaining highly specific and tailored ligands suitable for purifying a particular product target or for eliminating particular target impurities in a feed stream. Engineered affinity ligands according to the invention will bind a target with high specificity at a preselected binding condition and release the target at a preselected elution condition. The ligands are isolated by contacting a target with a multiplicity of polypeptides derived through variegation of the structure of a candidate binding domain, the variants (or analogues) including polypeptides favoring binding to the target under desired binding conditions and release from the target under elution conditions, where the binding and elution conditions differ according to one or more parameters, such as pH, temperature, concentration of salt or volume % of an organic solvent.
Type:
Grant
Filed:
March 21, 1997
Date of Patent:
December 4, 2001
Assignee:
Dyax Corp.
Inventors:
John Moore Maclennan, Robert Charles Ladner