Abstract: The present invention provides a method for synthesizing heat shock protein-peptide complexes comprising the steps of: adding a shock protein to a denatured protein matrix to bind the heat shock protein to the denatured protein matrix; and adding a complexing solution comprising a peptide to elute a heat shock protein-peptide complex. The present invention also provides a heat shock protein-peptide complex synthesized by the method of the invention. In addition the present invention provides an apparatus for synthesizing heat shock protein-peptide complexes comprising a heat shock protein complex bound to a denatured protein matrix.