Abstract: Composition comprising an aqueous solution of immune serum globulin and maltose, the amount of maltose being sufficient to inhibit "shedding" of the globulin with time.

Abstract: An improved check valve is disclosed for use in a system for sequentially delivering primary and secondary solutions through a common conduit. The check valve comprises a hollow housing with an inlet and an outlet connected to an enlarged intermediate portion. Sealed at its periphery in the enlarged portion is a flexible diaphragm characterized by having foraminous means in at least its outer portion and imperforate means in its central portion. The imperforate means is spaced from a valve seat surrounding the passage at the inner end of the inlet so that when the primary solution flows from the inlet to the outlet, it will pass freely around the imperforate means and through the foraminous means. When an increase in pressure, even though slight, is exerted against the diaphragm caused by flow of the secondary solution in the direction of the outlet to the inlet, the diaphragm moves towards the inlet causing the imperforate means to seal against the valve seat and stop the flow of the primary solution.

Abstract: Semi-solid preparation useful as a boticide and anthelmintic in horses and comprising an effective amount of trichlorfon dispersed in a mineral oil.

Abstract: The device comprises a closed container with a breathing tube and a means for variably adjusting the size of an aperture into the container. A transparent inclined tube is mounted on the container and houses a moveable air flow indicating member. The tube has a passage at the top leading into the container and a vent at the bottom of the tube allows air to enter to move the flow indicating member up the inclined tube. Air drawn from the container by inspiring through the breathing tube is replaced in part by air entering through the variable aperture means and by the air entering through the vent. By adjusting the size of the variable aperture, one can determine inspiratory capacity as correlated with the amount of air necessary to move the flow indicating member to the top of the tube or to an intermediate position in the tube.

Abstract: A glass article having an optical quality surface smoothness can be formed by molding a "silanol" glass material against a die having a molding surface which is a mirror of that required for the glass article, thereby obviating glass grinding and polishing steps. The article is prepared by first forming an anhydrous base glass comprising, in mole percent on the oxide basis, about 72 to 82% SiO.sub.2, 10 to 17% Na.sub.2 O and/or K.sub.2 O, and 5 to 15% of an oxide or oxides selected from ZnO and PbO. The base glass may include up to 5% Al.sub.2 O.sub.3 and up to 3% of B.sub.2 O.sub.3, BaO and/or MgO. The anhydrous base glass is then hydrated to include a water content ranging from 0.5 to 10% by weight to form a "silanol" glass. That glass is then molded against the die surface at a temperature ranging from about 250.degree. C. to 500.degree. C.

Abstract: An optical glass article having a desired surface figure and demonstrating maximum theoretical light transmission can be prepared by forming a base anhydrous glass of defined compositional ranges, hydrating the glass, partially dehydrating the glass at a temperature above the softening point and, using an inert gas, at a pressure above the vapor pressure of water contained therein, and then molding a preformed blank of the glass against a die having a desired optical quality surface figure, the molding being under conditions sufficient to transfer the surface figure of the die to the glass. The base glass comprises, in mole percent, about 70 to 82% SiO.sub.2, 10 to 17% Na.sub.2 O and/or K.sub.2 O, and 5 to 15% of an oxide or oxides selected from ZnO and PbO. The water content of the partially dehydrated glass ranges from about 3 to 8 wt. percent and the molded glass has a substantially uniform refractive index throughout its bulk.

Abstract: Immobilized enzyme composites comprising enzymes bonded to porous, high surface area support materials can be economically prepared by contacting "spent" composites with a solution of NaOCl to remove substantially all contaminants resulting from previous use of the composites and then reacting the cleaned support materials with a solution of active enzymes under conditions sufficient to permit the bonding of enzymes to the surfaces of the support materials.

Abstract: A glass article having an optical quality surface smoothness can be formed by molding a "silanol" glass material against a die having a molding surface which is a mirror of that required for the glass article, thereby obviating glass grinding and polishing steps. The article is prepared by first forming an anhydrous base glass comprising, in mole percent on the oxide basis, about 72 to 82% SiO.sub.2, 10 to 17% Na.sub.2 O and/or K.sub.2 O, and 5 to 15% of an oxide or oxides selected from ZnO and PbO. The base glass may include up to 5% Al.sub.2 O.sub.3 and up to 3% of B.sub.2 O.sub.3, BaO and/or MgO. The anhydrous base glass is then hydrated to include a water content ranging from 0.5 to 10% by weight to form a "silanol" glass. That glass is then molded against the die surface at a temperature ranging from about 250.degree. C. to 500.degree. C.

Abstract: Arylamine surface derivatives useful for the immobilization of biologically active proteins such as enzymes can be prepared by adsorbing p-phenylenediamine onto the surfaces of water-insoluble inorganic carriers having a relatively low surface isoelectric point.

Abstract: Biologically active proteins such as enzymes and antibodies can be chemically bonded to a variety of high surface area, inorganic supports by reacting the support surface with polymeric isocyanates and then reacting the coated surface with a dispersion of the proteins.BACKGROUND OF THE INVENTION1. FieldThe present invention is concerned generally with the immobilization of biologically active proteins and specifically with the chemical attachment of such proteins to high surface area inorganic supports.2. Prior ArtA variety of techniques have been devised to immobilize various biologically active proteins such as enzymes and antibodies onto and within high surface area inorganic supports. See, for example, U.S. Pat. No. 3,556,945 (enzymes adsorbed to porous glass); U.S. Pat. No. 3,804,719 (enzymes crosslinked within the pores of porous glass); U.S. Pat. No. 3,519,538 (enzymes coupled via intermediate silanes to various inorganics); U.S. Pat. No.

Abstract: Thyroxine binding globulin (TBG) and thyroxine (T.sub.4) can be efficiently removed fron serum containing TBG and T.sub.4 by contacting the serum with finely-divided alumina particles to adsorb a major portion of the T.sub.4 and then reacting the serum at an acidic pH with an anion exchange resin to extract substantially all TBG and the remaining T.sub.4.

Abstract: An aqueous suspension of porous glass particles having a closely controlled average particle size within the range of about 0.7 to about 3.0 microns. The controlled particle size porous glass particles are prepared by initially milling course porous glass particles to yield particles of about 10 microns or less, leaching those particles to remove contaminants and residues, rinsing the particles, and then mixing the particles with an aqueous solution and subjecting the mixture to at least two controlled sedimentation steps.

Abstract: Method of determining the concentration of thyroxine binding globulin (TBG) in a fluid sample. The method comprises the steps of adding to the sample an excess amount of thyroxine (T.sub.4) and then analyzing the sample for T.sub.4 via immunoassay technique in the presence and absence of a blocking agent to establish a binding differential. The differential is then correlated with a standard curve which relates known TBG concentrations to binding differentials obtained in a similar manner.

Abstract: Solidified, polar, micorbial growth medium containing 8-anilino-1-naphthalenesulfonic acid or a salt thereof. Microbial colonies grown on the medium can be detected fluorimetrically.

Abstract: Gel-like composition useful for separating and partitioning whole blood into serum and clot portions. The composition has a specific gravity between that of the serum and clot portions such that, when centrifuged in the presence of whole blood, the composition forms a chemical and physical barrier between the serum and clot portions. The composition comprises, in combination, a silicone fluid, an inert siliceous filler dispersed therein, and a network former consisting of a polysiloxane-polyoxyalkyl copolymer which stabilizes the composition by minimizing and/or avoiding "wet out" of the siliceous filler with time. The composition has a viscosity within the range of about 200,000 to about 600,000 centistokes, preferably within the range of about 350,000 to about 450,000 centistokes.

Abstract: An optical glass article having a desired surface figure and demonstrating maximum theoretical light transmission can be prepared by forming a base anhydrous glass of defined compositional ranges, hydrating the glass, partially dehydrating the glass at a temperature above the softening point and, using an inert gas, at a pressure above the vapor pressure of water contained therein, and then molding a preformed blank of the glass against a die having a desired optical quality surface figure, the molding being under conditions sufficient to transfer the surface figure of the die to the glass. The base glass comprises, in mole percent, about 70 to 82% SiO.sub.2, 10 to 17% Na.sub.2 O and/or K.sub.2 O, and 5 to 15% of an oxide or oxides selected from ZnO and PbO. The water content of the partially dehydrated glass ranges from about 3 to 8 wt. percent and the molded glass has a substantially uniform refractive index throughout its bulk.

Abstract: Method for determining the concentration of free (unbound) thyroid hormones (T.sub.3 or T.sub.4) in a fluid sample. The method comprises the steps of performing an immunoassay for total T.sub.3 or total T.sub.4 in the presence and absence of a blocking agent to establish a binding differential and then correlating that differential with a standard curve which relates known free thyroid hormone concentrations with binding differentials.

Abstract: A microbial growth medium for distinguishing Neisseria gonorrhoeae from Neisseria meningitidis. The medium comprises a gonococci and meningococci specific medium having incorporated therein a quantity of 8-anilino-1-naphthalene-sulfonic acid (ANS) or a salt thereof, at a concentration sufficient to permit the growth of the meningococci and inhibit the growth of the gonococci.

Abstract: Reagent and method for detecting the presence of hepatitis associated antigen (HAA) in blood serum. The reagent comprises anti-HAA antibodies coupled via an intermediate silane to a plurality of siliceous particles having a surface area of at least about 1 m.sup.2 /g and, if the particles are porous, an average pore diameter of at least about 1000 A. The test method comprises the steps of incubating a serum sample with the reagent to complex any HAA in the samplewith the antibodies of the reagent, incubating the reaction product with a solution of radio labelled anti-HAA antibodies to complex the labelled antibodies with any HAA that may be complexed to the reagent, separating the incubation products from the solution of labelled antibodies, and counting the radioactivity of either the separated products or the remaining solution to determine whether HAA was in the sample.

Abstract: Immunochemical composites consisting of saturating amounts of radio-labelled triiodothyronine (T.sub.3) or thyroxine (T.sub.4) complexed respectively to anti-T.sub.3 or anti-T.sub.4 antisera covalently coupled to suspendable porous glass particles via an intermediate silane coupling agent. The composites are pre-loaded into individual tubes used in solid phase radioimmunoassay of T.sub.3 or T.sub.4, requiring the addition of only test serum or standard, thereby minimizing chances of erroneous assay.