Patents Represented by Attorney, Agent or Law Firm James D. Darnley, Jr.
-
Patent number: 6096512Abstract: The present invention relates to a method for the isolation and expression of a glycosyltransferase enzyme for use in the synthesis of oligosaccharide or polysaccharide structures on glycoproteins, glycolipids, or as free molecules. The gene coding for the enzyme N-acetylgalactosaminyltransferase and the polypeptide sequence of the acceptor peptide for the enzyme N-acetylgalactosaminyltransferase have been isolated and used for the control of glycosylation of a protein.Type: GrantFiled: November 11, 1997Date of Patent: August 1, 2000Assignee: Pharmacia & Upjohn CompanyInventors: Ake P. Elhammer, Fred L. Homa
-
Patent number: 6013506Abstract: Disclosed are recombinant baculovirus expressing the gag, gp70, and gp85 genes of feline leukemia virus. Also disclosed are vaccines based on protein expressed from these recombinants. Also disclosed is a combined mucosal/parenteral inoculation method.Type: GrantFiled: June 5, 1995Date of Patent: January 11, 2000Inventors: Richard C. Wardley, Darrell R. Thomsen
-
Patent number: 6011011Abstract: The invention provides novel compositions useful for the sustained-release of bioactive proteins. More particularly, the present invention relates to novel suspensions of somatotropin and growth hormone releasing factor, in triacetin or polyethylene glycol. Also provided are methods of using these novel compositions as vehicles for the sustained or prolonged release of the bioactive protein or peptide.Type: GrantFiled: March 20, 1995Date of Patent: January 4, 2000Assignee: Pharmacia & Upjohn CompanyInventor: Michael J. Hageman
-
Patent number: 5910570Abstract: The present invention relates to a method for the isolation and expression of a glycosyltransferase enzyme for use in the synthesis of oligosaccharide or polysaccharide structures on glycoproteins, glycolipids, or as free molecules. The gene coding for the enzyme N-acetylgalactosaminyltransferase and the polypeptide sequence of the acceptor peptide for the enzyme N-acetylgalactosaminyltransferase have been isolated and used for the control of glycosylation of a protein.Type: GrantFiled: November 11, 1997Date of Patent: June 8, 1999Assignee: Pharmacia & Upjohn CompanyInventors: Ake P. Elhammer, Fred L. Homa
-
Patent number: 5849499Abstract: Provided are hybridomas for producing monoclonal antibodies against acetylated lysine residues of rbSt. The antibodies are specific to the presence of at least a single acetylated lysine in a protein and can differentiate between .alpha.-acetyl and .epsilon.-acetyl lysine as free amino acids.Type: GrantFiled: February 3, 1992Date of Patent: December 15, 1998Assignee: Pharmacia & Upjohn CompanyInventors: Diane Marie Hamlin, Gustavus Adolphus Walker
-
Patent number: 5833993Abstract: Disclosed are vaccines containing both a DNA sequence encoding FIV gag protein and a DNA sequence encoding FIV env protein. The gag and env proteins are preferably expressed by baculovirus expression systems containing the DNA sequences encoding the FIV env and gag proteins or in feline herpes virus vectors containing the DNA sequences encoding the FIV env and gag proteins. Also disclosed are combined mucosal/parenteral, mucosal/mucosal and parenteral/parenteral inoculation methods.Type: GrantFiled: October 25, 1996Date of Patent: November 10, 1998Assignee: Pharmacia & Upjohn CompanyInventors: Richard C. Wardley, David E. Lowery
-
Patent number: 5759852Abstract: Disclosed are expression vectors useful as vectors in recombinant methods to facilitate expression of exogenous genes in E. coli. Specifically, the disclosed expression vector has the following elements in operable linkage: the PL6m promoter, the TAT32 ribosome binding site and a gene encoding a heterologous polypeptide, Also disclosed are E. coli host cells transformed with this expression vector.Type: GrantFiled: June 27, 1994Date of Patent: June 2, 1998Assignee: Pharmacia & UpJohn CompanyInventors: Richard J. Kirschner, John Edward Mott, Frances M. Eckenrode, David Paul Brunner
-
Patent number: 5721132Abstract: A modified gene coding for the human D4 dopamine receptor has been synthesized by chemo-enzymatic methods. The nucleotide sequence of the D4 dopamine receptor gene was changed to reduce the G+C content and to eliminate intronic sequences, while maintaining the published amino acid sequence. Using gene splicing by overlap extension and PCR amplification of long oligonucleotides (>200 bases), 3 synthetic fragments of about 400 base pairs each were amplified, from which the full length gene was assembled. Stable expression of this gene has been achieved in CHO-K1 cells, using an inducible expression system, and in HEK293 cells.Type: GrantFiled: May 3, 1995Date of Patent: February 24, 1998Assignee: The Upjohn CompanyInventors: Christopher L. Chio, Rita M. Huff
-
Patent number: 5718901Abstract: The present invention provides recombinant DNA molecules comprising a sequence encoding a feline calicivirus polypeptide, particularly the capsid protein, host cells transformed by recombinant DNA molecule sequences, and the capsid polypeptides. The present invention also provides subunit vaccines and multivalent vaccines for FCV.Type: GrantFiled: June 5, 1995Date of Patent: February 17, 1998Assignee: Pharmacia & Upjohn CompanyInventors: Richard Calvert Wardley, Leonard Edwin Post
-
Patent number: 5716822Abstract: The present invention provides recombinant DNA molecules comprising a sequence encoding a feline calicivirus polypeptide, particularly the capsid protein, host cells transformed by said recombinant DNA molecule sequences, and the capsid polypeptides.Type: GrantFiled: June 5, 1995Date of Patent: February 10, 1998Assignee: Pharmacia & Upjohn CompanyInventors: Richard Calvert Wardley, Leonard Edwin Post
-
Patent number: 5698418Abstract: The present invention provides an inexpensive fermentation medium for growing microorganisms. Also provided are fermentation media and methods for producing norleucine by growing E. coli thereon, fermentation media and methods for incorporating norleucine into polypeptides expressed by microorganisms grown thereon, and fermentation media and methods for preventing the incorporation of norleucine into polypeptides expressed by microorganisms grown thereon. Also provided are bovine somatotropin analogs.Type: GrantFiled: June 6, 1995Date of Patent: December 16, 1997Assignee: Pharmacia & Upjohn CompanyInventors: David P. Brunner, Gary C. Harbour, Richard J. Kirschner, James F. Pinner, Robert L. Garlick
-
Patent number: 5674709Abstract: The present invention provides recombinant DNA molecules comprising a sequence encoding a pseudorabies virus (PRV) glycoprotein selected from the group consisting of gI, gp50, and gp63, host cells transformed by said recombinant DNA molecule sequences, the gI, gp50 and gp63 polypeptides. The present invention also provides subunit vaccines for PRY, methods for protecting animals against PRV infection and methods for distinguishing between infected and vaccinated animals.Type: GrantFiled: June 21, 1994Date of Patent: October 7, 1997Assignee: Pharmacia & Upjohn CompanyInventors: Erik Aivars Petrovskis, Leonard Edwin Post, James G. Timmins
-
Patent number: 5663305Abstract: The present invention provides analogs of animal somatotropins particularly with changes in the amino acids between residue 96 to 133 which are changed to reduce hydrophobicity or helical stability.Type: GrantFiled: December 29, 1994Date of Patent: September 2, 1997Assignee: The Upjohn CompanyInventors: Sherwood Russ Lehrman, Henry A. Havel, Jody L. Tuls, Scott M. Plaisted, David N. Brems
-
Patent number: 5643940Abstract: This invention concerns a method for killing internal parasites, especially nematodes, trematodes and cestodes affecting warm blooded animals such as sheep, cattle, swine, goats, dogs, cats, horses and humans as well as poultry by administering an effective amount of dioxapyrrolomycin of the formula I. Anthelmintic compositions of dioxapyrrolomycin and an improvement in the process of preparation of dioxapyrrolomycin are also provided.Type: GrantFiled: May 1, 1995Date of Patent: July 1, 1997Assignee: The Upjohn CompanyInventors: George Anthony Conder, Ming-Shang Thomas Kuo, Vincent P. Marshall, Raymond John Zielinski
-
Patent number: 5631227Abstract: The present invention provides analogs of somatotropins wherein the asparagine residue corresponding to position 99 of a bovine somatotropin is replaced with a compound selected from the group consisting of proline, aspartic acid, glutamic acid, serine, glycine, serine-serine or serine-aspartic acid. Particularly preferred analogs are bovine somatotropin and their use to increase milk production.Type: GrantFiled: June 5, 1995Date of Patent: May 20, 1997Assignee: The Upjohn CompanyInventors: Gary C. Harbour, John G. Hoogerheide, Robert L. Garlick, Stephen B. Lyle, John E. Mott
-
Patent number: 5622845Abstract: The present invention provides an inexpensive fermentation medium for growing microorganisms. Also provided are fermentation media and methods for producing norleucine by growing E. coli thereon, fermentation media and methods for incorporating norleucine into polypeptides expressed by microorganisms grown thereon, and fermentation media and methods for preventing the incorporation of norleucine into polypeptides expressed by microorganisms grown thereon. Also provided are bovine somatotropin analogs.Type: GrantFiled: June 6, 1995Date of Patent: April 22, 1997Assignee: The Upjohn CompanyInventors: David P. Brunner, Gary C. Harbour, Richard J. Kirschner, James F. Pinner, Robert L. Garlick
-
Patent number: 5601974Abstract: Provided are methods for determining whether a vaccinated animal is uninfected or infected with a virulent wild-type virus. Methods, vaccines and viruses are disclosed that are useful to seratologically distinguish infected from uninfected animals in populations of animals vaccinated with a properly incapacitated virus lacking an antigen of the wild-type virus.Type: GrantFiled: December 19, 1994Date of Patent: February 11, 1997Assignee: The Upjohn CompanyInventors: Leonard E. Post, Darrell R. Thomsen
-
Patent number: 5594115Abstract: Recombinant fusion proteins which comprise a biologically active polypeptide portion and metal chelating peptide are disclosed. The fusion proteins contain metal chelating peptides which have at least three and preferably six alternating histidine residues. Fusion proteins which contain metal chelating peptides that are substrates for dipeptidylpeptidase I are also disclosed. Additionally, a method of obtaining desired polypeptides by producing recombinant fusion proteins and purifying them with immobilized metal ions is disclosed. A kit for purifying desired polypeptides is also disclosed.Type: GrantFiled: December 29, 1994Date of Patent: January 14, 1997Assignee: Pharmacia & Upjohn CompanyInventor: Satish K. Sharma
-
Patent number: 5583106Abstract: This invention provides a method of using somatotropin to enhance the reproductive performance in cattle by commencing administration during the early postpartum period. Following one aspect of the invention, somatotropin (5 mg/day) is administered starting at five days postpartum, continuing until the animal becomes pregnant or until about 100 days postpartum.Type: GrantFiled: February 8, 1994Date of Patent: December 10, 1996Assignee: The Upjohn CompanyInventor: Edward P. Stanisiewski
-
Patent number: 5510256Abstract: The present invention is based upon the discovery that proteins made from genes that include the CD4 sequence in its cDNA can make additional polypeptides as a result of internal translation initiation. This invention is thus directed to DNA sequences which eliminate internal initiation expression in sCD4.Type: GrantFiled: December 29, 1994Date of Patent: April 23, 1996Assignee: The Upjohn CompanyInventors: Richard J. Kirschner, John E. Mott, Frances M. Eckenrode, David P. Brunner