Patents Represented by Attorney, Agent or Law Firm Janelle S. Graeter
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Patent number: 6770440Abstract: Oligonucleotide primer sequences have been designed which are capable of uniquely amplifying DNA from Toxoplasma gondii when utilized in a polymerase chain reaction assay. In addition, a probe effective for detection of amplification products, particularly in a fluorogenic real time assay system has also been designed. The primers and probe are derived from the internal transcribed spacer 1 (ITS1) region of the T. gondii 18S rRNA gene.Type: GrantFiled: May 31, 2001Date of Patent: August 3, 2004Assignee: The United States of America as represented by the Secretary of AgricultureInventors: Joan K. Lunney, Luis H. Jauregui, James Higgins, Dante S. Zarlenga, Jitender P. Dubey
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Patent number: 6689383Abstract: Chromium is an essential element which has been shown to have beneficial effects on carbohydrate and lipid metabolism. Often, dietary intake is suboptimal, and nutritional supplements may not be effective due to poor absorption in the gastrointestinal tract. A novel chromium-histidine complex has been prepared which significantly improves absorption capability over current available chromium nutritional supplements. The complex is safe, easily prepared and may be combined with an ingestible carrier for consumption as a tablet, capsule, aqueous solution, food or food product.Type: GrantFiled: October 8, 1999Date of Patent: February 10, 2004Assignee: The United States of America as represented by the Secretary of AgricultureInventors: Richard A. Anderson, Marilyn M. Polansky, Noella A. Bryden
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Patent number: 6503698Abstract: A method for the cryopreservation of zona pellucida-intact, early-stage swine embryos, morulae and blastocysts includes centrifuging the embryos prior to cryopreservation in order to remove intracytoplasmic lipids. The lipids are localized external to the embryo proper inside the zona pellucida, thus neutralizing harmful effects which prevent subsequent embryological development. The embryos may be cryopreserved by vitrification or conventional freezing. After recovery and rehydration, zona pellucidae are removed, and intact, viable embryos are transferred to recipient females.Type: GrantFiled: June 16, 2000Date of Patent: January 7, 2003Assignee: The United States of America as represented by the Secretary of AgricultureInventors: John R. Dobrinsky, Hiroshi Nagashima
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Patent number: 6500425Abstract: The present invention is drawn to biological control of plant pathogens (e.g. either preharvest or postharvest diseases) on agricultural commodities (such as fruits, vegetables, cereals, grains, nuts, seeds and silage) by use of at least one microorganism which is an antagonist against plant pathogens.Type: GrantFiled: September 28, 1994Date of Patent: December 31, 2002Assignee: The United States of America as represented by the Secretary of AgricultureInventors: Randy J. McLaughlin, Charles L. Wilson, Edo Chalutz
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Patent number: 6476192Abstract: Antigens for the detection of antibodies to Neospora parasites for the diagnosis of neosporosis have been identified. Recombinant antigens may be produced by expression of DNA sequences derived from Neospora caninum. Both antigens are capable of detecting antibody responses in animals experimentally inoculated with N. caninum but show no evidence of cross-reactivity with serum from animals inoculated with closely related parasites such as Toxoplasma gondii or Sarcocystis species.Type: GrantFiled: April 15, 1996Date of Patent: November 5, 2002Inventors: Nicola C. Lally, Mark C. Jenkins, Jitender P. Dubey
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Patent number: 6451984Abstract: A stable chicken hybridoma secreting a monoclonal antibody (mAb) that detects the conoid structure of Eimeria acervulina (E. acervulina) sporozoites has been developed. The hybridoma is made by fusing a thymidine kinase (TK)-deficient chicken myeloma with spleen cells from chickens immunized with sporozoite antigen. The monoclonal antibody recognizes sporozoite proteins on the conoid of the anterior tip of E. acervulina sporozoites. The monoclonal antibody has been shown to inhibit the invasion of sporozoites into CD8+ T cells in vitro thereby indicating its role in the recognition of host cells during the invasion process following infection with Eimeria parasites.Type: GrantFiled: September 8, 1995Date of Patent: September 17, 2002Assignee: The United States of America as represented by the Secretary of AgricultureInventors: Hyun S. Lillehoj, B. Nichols Majorie
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Patent number: 6442920Abstract: An apparatus for the mechanical harvesting of tree fruits has been designed which has the capability of providing fruit suitable for the fresh market. The harvester features an impactor for dislodging fruit from a tree limb, a unique positioning system, a collecting conveyor utilizing energy-absorbing materials for minimizing fruit damage due to the impact of falling fruit, a transport conveyor which moves the harvested fruit to a revolving collecting bin, and bin-filling mechanisms which gently deposit harvested fruit in the collecting bin.Type: GrantFiled: June 7, 2001Date of Patent: September 3, 2002Assignee: The United States of America as represented by the Secretary of AgricultureInventors: Donald L. Peterson, Scott D. Wolford
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Patent number: 6410223Abstract: A novel polymerase chain reaction (PCR) technique which can specifically detect viable cells of a target cell or microorganism has been developed. The method combines a biological preamplification on growth medium with direct PCR and eliminates DNA extraction steps required for conventional PCR methods.Type: GrantFiled: November 4, 1994Date of Patent: June 25, 2002Assignee: The United States of America as represented by the Secretary of AgricultureInventors: Norman W. Schaad, Nikolas J. Panopoulos, Efstathios Hatziloukas
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Patent number: 6372241Abstract: A novel dispenser for the release of insect attractants has been invented for baiting insect traps. The dispenser is comprised of a flexible support coated with a polyvinylchloride (PVC)-insect attractant mixture. A PVC/twine dispenser has been found to be particularly effective for the release of the gypsy moth pheromone, (+)-disparlure.Type: GrantFiled: May 24, 1994Date of Patent: April 16, 2002Assignee: The United States of America as represented by the Secretary of AgricultureInventors: Barbara A. Leonhardt, E. David DeVilbiss, Victor C. Mastro
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Patent number: 6210931Abstract: Circular RNAs may be synthesized by inserting DNA fragments into a plasmid containing sequences having the capability of spontaneous cleavage and self-circularization. Insertion of the DNA fragments allows RNAs of predetermined size to be constructed. In addition, a two-dimensional polyacrylamide gel electrophoresis system having a second dimension more highly cross-linked than the first dimension permits the separation and analysis as well as the precise sizing of both linear and circular RNAs produced by the synthetic method.Type: GrantFiled: November 30, 1998Date of Patent: April 3, 2001Assignee: The United States of America as represented by the Secretary of AgricultureInventors: Paul A. Feldstein, Robert A. Owens, Laurene Levy, John W. Randles
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Patent number: 6180378Abstract: Immobilized bioactive protein compositions are prepared containing a bioactive protein such as an enzyme intercalated into galleries of a phyllosilicate, and a crosslinking compound crosslinking the phyllosilicate and the bioactive protein. The phyllosilicate may contain sodium or alkylammonium ions and be montmorillonite. The protein may be lipoxygenase, and crosslinking compounds include tetramethyl orthosilicate, tetraethoxy silicate, propyltrimethoxy silicate, polydimethylortho silicate and methyltrimethoxy silicate. The composition is prepared by delaminating a sodium-saturated phyllosilicate, mixing a bioactive protein with the delaminated phyllosilicate and crosslinking with a crosslinking compound. After crosslinking, the composition may be vacuum dried and ground.Type: GrantFiled: January 29, 1999Date of Patent: January 30, 2001Assignee: The United States of America as represented by the Secretary of AgricultureInventors: Siyuan Shen, An-Fei Hsu, Thomas A. Foglia, Shu-I Tu
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Patent number: 6177073Abstract: Compositions and methods are provided for attracting and subsequently trapping and/or killing adults of the Asian longhorned beetle, Anoplophora glabripennis, or interfering with their reproduction. The aggregation pheromones, 4-(n-heptyloxy)butanal and 4-(n-heptyloxy)butan-1-one, in combination with a trap or toxicant, provide means for monitoring, killing or inhibiting the reproduction of these insects.Type: GrantFiled: July 7, 1999Date of Patent: January 23, 2001Assignee: The United States of America as represented by the Secretary of the AgricultureInventors: Aijun Zhang, James E. Oliver, Jeffrey R. Aldrich
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Patent number: 6143337Abstract: High-quality pectins may be extracted from pectin-containing plant materials with microwave energy under pressure. The pectins are characterized by increased molecular weight, size and intrinsic viscosity when compared to pectin extracted by conventional heating techniques.Type: GrantFiled: April 30, 1999Date of Patent: November 7, 2000Assignee: The United States of America as represented by the Secretary of AgricultureInventors: Marshall L. Fishman, Hoa K. Chau
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Patent number: 6143565Abstract: A stable transformed insect cell line has been achieved by infecting an insect cell culture with fluid obtained from a female parasitoid wasp, such as Glyptapanteles indiensis. The fluid contains polydnavirus DNA capable of integrating into the host cell genome. Susceptible insect cells include both Lepidopteran and Coleopteran cells.Type: GrantFiled: August 22, 1997Date of Patent: November 7, 2000Assignee: The United States of America as represented by the Secretary of AgricultureInventors: Edward M. Dougherty, Terry A. McKelvey, Donald A. Stoltz, Dwight E. Lynn, Dawn E. Gundersen-Rindal
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Patent number: 6090398Abstract: Diabroticite insects, in particular the corn rootworm, are major pests of corn as well as a variety of other agricultural crops. The invention describes an aqueous composition which is effective as an insecticide for the control of Diabroticite insects and contains a photoactive xanthene dye, such as phloxine B, as a toxicant and a cucurbitacin-containing feeding stimulant. Additives such as a starch thickener and an adherent may also be included in the composition.Type: GrantFiled: July 15, 1999Date of Patent: July 18, 2000Assignee: The United States of America as represented by the Secretary of AgricultureInventors: Robert F. W. Schroder, Albert B. Demilo, Chang Joo Lee
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Patent number: 6087165Abstract: Recombinant baculoviruses were prepared which contain sequences expressing peptide hormones which cause lack of feeding in Helicoverpa zea (corn earworm) and related pestw. The peptides were isolated from H. zea and identified as helicokinins. When present in sufficient amounts in the insect, weight and water loss and the eventual death of the insect occurs.Type: GrantFiled: February 26, 1999Date of Patent: July 11, 2000Assignee: The United States of America as represented by the Secretary of AgricultureInventors: Ashok K. Raina, Robert F. Leclerc, Vikram N. Vakharia
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Patent number: 6037374Abstract: Gel compositions of formic acid and a gelling agent such as fumed silica or polyacrylic acid have been prepared for controlling parasitic mites of honey bees. The gels provide slow-release capability and have been found effective against both Varroa jacobsoni and Acarapis woodi. They are advantageous in that they have improved handling characteristics and are safer than liquid formic acid, which is quite corrosive.Type: GrantFiled: November 19, 1997Date of Patent: March 14, 2000Assignee: The United States of America as represented by the Secretary of AgricultureInventors: Jan P. Kochansky, Hachiro Shimanuki, Mark Francis Feldlaufer, Jeffery S. Pettis
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Patent number: 6023855Abstract: The invention provides an air-assisted wiping device for use in a continuous-spray dryer. The air-assisted wiping device rotates inside the housing to continuously wipe heavy sticky powder from the walls of the housing using a wiper blade. The air-assisted wiping device includes an elongated hollow plenum having a flow-directing slot disposed along its length. Air received into the elongated hollow plenum is passed through the flow-directing slot onto a wiper blade, which is coupled to the elongated hollow plenum. The air discharged onto the wiper blade removes powder that collects on the leading side of the wiper blade.Type: GrantFiled: November 27, 1996Date of Patent: February 15, 2000Assignee: The United States of America as represented by the Secretary of AgricultureInventors: Neil M. Goldberg, Charles I. Onwulata, Philip Wayne Smith, Greg Tomes
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Patent number: 6017713Abstract: An in vitro system has been developed capable of effectively predicting the iron (Fe) availability of foods and food products. The system utilizes the formation of ferritin in intestinal epithelial cells as an indicator of Fe uptake from peptic and intestinal food digests.Type: GrantFiled: March 12, 1999Date of Patent: January 25, 2000Assignee: The United States of America, as represented by the Secretary of AgricultureInventor: Raymond P Glahn
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Patent number: 6017752Abstract: An isolate from the surface of apple leaves, Pseudomonas syringae pv. lachrymans having the deposited accession number NRRL B-18739, exhibits antifungal properties. The organisms have utility in methods for controlling postharvest disease in agricultural commodities caused by fungal pathogens.Type: GrantFiled: November 27, 1990Date of Patent: January 25, 2000Assignee: The United States of America as represented by the Secretary of AgricultureInventors: Wojciech J Janisiewicz, Leonard Yourman