Abstract: The invention relates to an isolated thermostable glucoamylase derived from Talaromyces emersonii suitable for starch conversion processes.

Abstract: The present invention relates to isolated polypeptides having glucanotransferase activity and isolated nucleic acid sequences encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the nucleic acid sequences as well as methods for producing and using the polypeptides.

Abstract: The present invention relates to a particle comprising an active dispersed in a visco-elastic liquid matrix having a &eegr;′ and a &eegr;″ between 103 to 1014 Pa measured in a cone-and-plate rheometer at 25° C. and a sinusoidal frequencies &ohgr; of 1 Hz.

Abstract: The invention relates to a ellulose film comprising microfibrillated cellulose and to the use of it for screening of a biological compound and nucleic acids encoding a biological compound.

Abstract: A process for producing paper materials having improved wet strength. This process involves (a) preparing a suspension of unbleached or semi-bleached chemical or semichemical pulp or pulp from recycled fibers; (b) treating the pulp with a phenol-oxidizing enzyme and a mediator; and (c) de-watering the treated pulp in a paper making machine to remove process water and produce the paper material. Preferably, the paper material is heated after the completion of step (c). By the process of the invention, the wet strength of paper materials can be improved without using wet strength resins which makes the product more easily re-used. Further disclosed is a process for producing corrugated paperboard or corrugated containers.

Abstract: The present invention relates to isolated polypeptides having branching enzyme activity and isolated nucleic acid sequences encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the nucleic acid sequences as well as methods for producing and using the polypeptides.

Abstract: The present invention relates to enzyme-containing granules comprising (a) an enzyme and (b) a core which intrinsically is capable of absorbing at least 5% w/w (based on the weight of the core) of water and to processes for the production of such granules comprises (a) contacting absorbent cores, capable of absorbing at least 5% w/w (based on the weight of the core) of water, with a liquid medium, such as an aqueous medium, containing an enzyme in dissolved and/or dispersed form, the amount of the liquid medium employed being such that substantially no attendant agglomeration of the resulting product occurs; and (b) at least partially removing volatile components of the liquid medium from the resulting product.

Abstract: A microfiltration process of a fermentation-derived product comprising adding activated carbon to a solution of the fermentation-derived product prior to or during the microfiltration process at a microfiltration process temperature of from 25° C. to 65° C.

Abstract: In the production of pulp and paper from starch-containing paper, the deinking effect can be improved by including treatment with both a starch-degrading enzyme and a pectate lyase. The process comprises enzyme treatment during or after disintegration of the paper to produce pulp, followed by separation of ink particles.

Abstract: The invention relates to a process for treatment of cellulosic material, as for example, knitted or woven cotton fabric, comprising the steps of preparing an aqueous enzyme solution comprising pectinase, treating the cellulosic material with an effective amount of the aqueous enzyme solution under alkaline scouring conditions; e.g., pH of 9 or above and a temperature of 50° C. or above, in a low calcium or calcium-free environment, yielding a modification of the cellulosic material such that exhibits an enhanced respond to a subsequent chemical treatment.

Abstract: The present invention relates to isolated polypeptides having polypeptide having 2,6-&bgr;-D-fructan hydrolase activity and isolated nucleic acid sequences encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the nucleic acid sequences as well as methods for producing and using the polypeptides.

Abstract: A process for making DNA libraries in filamentous fungal cells using a novel cloned gene involved in the mismatch repair system of filamentous fungal cells.

Abstract: A method for in vivo production of a library in cells comprising a multitude of mutated genetic elements, wherein an error-prone polymerase is used in each ancestral cell to replicate all or a part of a genetic element independently of the host chromosomal replication machinery. The genetic element comprises i) an origin of replication from which replication is initiated, ii) optionally a genetic marker, e.g. a gene conferring resistance towards an antibiotic, iii) a gene encoding the polypeptide of interest. Also methods for the generation of a DNA sequence encoding a desired variant of a polypeptide of interest, and for the determination of such a DNA sequence are described.

Abstract: The inventors have discovered some striking, and not previously predicted structural similarities and differences between the structure of Novamyl and the reported structures of CGTases, and based on this they have constructed variants of maltogenic alpha-amylase having CGTase activity and variants of CGTase having maltogenic alpha-amylase activity. Further, on the basis of sequence homology between Novamyl® and CGTases, the inventors have constructed hybrid enzymes with one or more improvements to specific properties of the parent enzymes, using recombinant DNA methodology.

Abstract: Sanitary paper with improved softness (lower stiffness) can be obtained without significant loss of paper strength by using a papermaking pulp which is treated with a certain type of cellulase component. The cellulase component in question is characterized by not containing a cellulose-binding domain (CBD), and is more effective for making softer sanitary paper than a conventional cellulase preparation which contains a mixture of various cellulase components with and without a CBD.

Abstract: It has been found by a screening assay that XET activity is produced by an overwhelming array of phylogenetically dispersed microorganisms. Accordingly, the present invention relates to a xyloglucan endotransglycosylase preparation which is producible by cultivation of a microorganism expressing an XET.

Abstract: The present invention relates to a starch conversion process of the type which includes a debranching step wherein an isoamylase being active at the process conditions prevailing is used for debranching the starch and to the use of thermostable isoamylases for starch conversion. The invention further relates to an isolated isoamylase obtained from a strain of the genus Rhodothermus and to cloned DNA sequences encoding isoamylases derived from a strain of Rhodothermus or Sulfolobus, to expression vectors comprising said DNA sequence, host cells comprising such expression vectors, and finally to methods for producing said isoamylases.

Abstract: The present invention relates to a method of constructing a variant of a parent Termamyl-like &agr;-amylase, which variant has &agr;-amylase activity and at least one altered property as compared to the parent &agr;-amylase, comprises i) analysing the structure of the parent Termamyl-like &agr;-amylase to identify at least one amino acid residue or at least one structural part of the Termamyl-like &agr;-amylase structure, which amino acid residue or structural part is believed to be of relevance for altering the property of the parent Termamyl-like &agr;-amylase (as evaluated on the basis of structural or functional considerations), ii) constructing a Termamyl-like &agr;-amylase variant, which as compared to the parent Termamyl-like &agr;-amylase, has been modified in the amino acid residue or structural part identified in i) so as to alter the property, and, optionally, iii) testing the resulting Termamyl-like &agr;-amylase variant with respect to the property in question.

Abstract: The invention relates to a variant of a parent Termamyl-like a-amylase, which variant has a-amylase activity and exhibits an alteration in at least one of the following properties relative to parent a-amylase: substrate specificity, substrate binding, substrate cleavage pattern, thermal stability, pH/activity profile, pH/stability profile, stability towards oxidation, Ca2+ dependency and specific activity.

Abstract: The present invention relates to transglutaminases and transglutaminase preparations obtained from lower fungi belonging to the class Oomycetes. Unprecedented high-level expression is achievable by growing these coenocytium forming organisms, especially the strains Pythium sp., Pythium irregulare, Pythium dissotocum, Pythium periilum (or P. periplocum), Pythium torulosum, Pythium ultimum, Pythium aphanidermatum, Phytophthora cactorum, Phytophthora palmivora, Phytophthora porri, Phytophthora infestans, Phytoph-thora megasperma, Phytophthora cinnamomi and Phytophthora cryptogea. Arecombinant transglutaminase has been cloned and expressed, the enzyme and enzyme preparations being useful for cross-linking proteins, e.g. in flour, baked products, meat products, fish products, cosmetics, cheese, milk products, gelled food products and leather finishing, or as a glutaminase, e.g. in bread and other baked glutein-containing food products.