Abstract: A method of synthesis of a material corresponding to the general formula: R3—X—H characterized in that it comprises a material corresponding to the following general formula: being cleaved as indicated enzymatically or non-enzymatically using acid catalysis in the presence of a nucleophile, wherein: R1 represents a group providing the site for exo-enzyme or acid hydrolysis; R2 represents an intermediate linked to a solid support; R3 represents a carbohydrate, (oligo-)saccharide, (glyco-)peptide, (glyco-)lipid or an organic molecule which is at least one of heterocyclic and aromatic in structure; X represents O, N(H), N(R″), C(O)O, S, C(O)N(H) or C(O)N(R″), R″ being a non-interfering group; and Support represents a solid support.

Abstract: Recombinant active glucocerebrosidase is produced by a eukaryotic cell. Also, a cell includes nucleic acid encoding enzymatically active glucocerebrosidase; also a eukaryotic organism contains such a cell. Also, a method for producing enzymatically active glucocerebrosidase includes steps of introducing glucocerebrosidase-encoding nucleic acid into a eukaryotic cell, causing the cell to express glucocerebrosidase, and purifying the glucocerebrosidase from the cell.

Abstract: Inter alia, an enzyme, a 2-hydroxy carboxylic acid dehydrogenase, characterised in that it is obtainable from Lactobacillus delbrueckii ssp. Bulgaricus and in that it catalyses the production of (R)-hydroxy derivatives of 2-keto acids, which are unsubstituted in the 3-position, but which may be substituted in the 4-position or beyond is disclosed.