Abstract: The present invention relates to novel lipopeptide compounds. The invention also relates to pharmaceutical compositions of these compounds and methods of using these compounds as antibacterial compounds. The invention also relates to methods of producing these novel lipopeptide compounds and intermediates used in producing these compounds.
Type:
Grant
Filed:
December 15, 2000
Date of Patent:
September 21, 2004
Assignee:
Cubist Pharmaceuticals, Inc.
Inventors:
Jason Hill, Ian Parr, Michael Morytko, Jim Siedlecki, Xian Yang Yu, Jared Silverman, Dennis Keith, John Finn, Dale Christensen, Tsvetelina Lazarova, Alan D. Watson, Yan Zhang
Abstract: The invention discloses highly purified daptomycin and to pharmaceutical compositions comprising this compound. The invention discloses a method of purifying daptomycin comprising the sequential steps of anion exchange chromatography, hydrophobic interaction chromatography and anion exchange chromatography. The invention also discloses a method of purifying daptomycin by modified buffer enhanced anion exchange chromatography. The invention also discloses an improved method for producing daptomycin by fermentation of Streptomyces roseosporus. The invention also discloses high pressure liquid chromatography methods for analysis of daptomycin purity. The invention also discloses lipopeptide micelles and methods of making the micelles. The invention also discloses methods of using lipopeptide micelles for purifying lipopeptide antibiotics, such as daptomycin. The invention also discloses using lipopeptide micelles therapeutically.
Type:
Grant
Filed:
November 28, 2000
Date of Patent:
February 24, 2004
Assignee:
Cubist Pharmaceuticals, Inc.
Inventors:
Thomas J. Kelleher, Jan-Ji Lai, Joseph P. DeCourcey, Paul Lynch, Maurizio Zenoni, Auro Tagliani