Abstract: Recombinant baculoviruses were prepared which contain sequences expressing peptide hormones which cause lack of feeding in Helicoverpa zea (corn earworm) and related pestw. The peptides were isolated from H. zea and identified as helicokinins. When present in sufficient amounts in the insect, weight and water loss and the eventual death of the insect occurs.
Type:
Grant
Filed:
February 26, 1999
Date of Patent:
July 11, 2000
Assignee:
The United States of America as represented by the Secretary of Agriculture
Inventors:
Ashok K. Raina, Robert F. Leclerc, Vikram N. Vakharia
Abstract: Hybridoma cell lines have been produced which secrete monoclonal antibodies that bind hygromycin B and are effective to detect hygromycin B levels as low as about 0.7 ppm. These monoclonal antibodies may be used for the detection and quantitative determination of trace amounts of hygromycin B in samples, especially in animal tissue and feed material.
Type:
Grant
Filed:
March 14, 1995
Date of Patent:
April 15, 1997
Assignee:
The United States of America as represented by the Secretary of Agriculture
Inventors:
Carol Kamps-Holtzapple, Larry H. Stanker, John R. DeLoach