Abstract: A method of enhancing the migration and proliferation of keratinocytes in wound healing or in the growth of artificial skin grown in vitro. The wound is contacted with (a) an effective amount of purified Clostridiopeptidase A collagenase that is substantially free from other proteinases and (b) an amount of a growth factor that increases the effects of said collagenase. The artificial skin is grown upon biomatrices previously synthesized by living cells and digested with Clostridiopeptidase A collagenase while in the presence of (a) an effective concentration of purified Clostridiopeptidase A collagenase substantially free from other proteinases and (b) a growth factor in a concentration effective to increase the effectiveness of said collagenase.

Abstract: Densely packed cells growing rapidly in substrate-attached culture are gently released from the substrate by enzymatic treatment to form aggregates ranging from 20 .mu. to 500 .mu. in diameter. These aggregates are then transferred to fresh medium in agitated liquid suspension. The cells thereby continue to grow in aggregates whereby a normally anchorage-dependent estabished mammalian cell line is rapidly adapted to suspension culture.