Patents Represented by Attorney John M. Polo

Find this attorney in the Justia Lawyer Directory

  • Methods and materials for increasing expression of recombinant polypeptides
    Patent number: 7993915
    Abstract: The present invention provides novel methods and materials for increasing the expression of recombinant polypeptides. Methods and materials of the invention allow increased expression of transcription units that include recombinant DNA sequences which encode polypeptides of interest. The present invention provides expression vectors which contain multiple copies of a transcription unit encoding a polypeptide of interest separated by at least one selective marker gene and methods for sequentially transforming or transfecting host cells with expression vectors to increase transcription unit dosage and expression.
    Type: Grant
    Filed: February 9, 2007
    Date of Patent: August 9, 2011
    Assignee: XOMA Technology Ltd.
    Inventor: Arnold M. Horwitz
  • Eukaryotic signal sequences for polypeptide expression and polypeptide display libraries
    Patent number: 7977068
    Abstract: The present invention generally relates to methods and compositions for expressing proteins or polypeptides in prokaryotic hosts using eukaryotic signal sequences.
    Type: Grant
    Filed: January 25, 2008
    Date of Patent: July 12, 2011
    Assignee: XOMA Technology Ltd.
    Inventors: Jeff Gray, Joe Buechler, Uday Kumar Veeramallu
  • Eukaryotic signal sequences for polypeptide expression and polypeptide display libraries
    Patent number: 7972811
    Abstract: The present invention generally relates to methods and compositions for expressing proteins or polypeptides in prokaryotic hosts using eukaryotic signal sequences.
    Type: Grant
    Filed: January 28, 2008
    Date of Patent: July 5, 2011
    Assignee: XOMA Technology Ltd.
    Inventors: Jeff Gray, Joe Buechler, Uday Kumar Veeramallu
  • Methods and materials for expression of a recombinant protein
    Patent number: 7794976
    Abstract: Recombinant expression vectors are provided comprising a 3?UTR of a light chain and an Epstein-Barr virus origin of replication. Also provided are host cells comprising such vectors and methods of producing recombinant protein with such vectors. Additional methods of producing a recombinant protein involve contacting cells with a first and second vector, each of which encode a different polypeptide chain, and wherein the second vector is present in an amount which is about 1.5 to 2.5 times as much as that of the first vector. Cells also can be transfected with a recombinant transient expression vector encoding a protein and are cultured in a medium in a membrane-enhanced culturing vessel to produce recombinant protein.
    Type: Grant
    Filed: July 31, 2007
    Date of Patent: September 14, 2010
    Assignee: XOMA Technology Ltd.
    Inventors: Masahisa Handa, Arnold H. Horwitz, Robyn Cotter, Eddie Batista
  • Methods for recombinant peptide production
    Patent number: 7749731
    Abstract: The present invention provides improved methods for the production of recombinant peptides from bacterial cells.
    Type: Grant
    Filed: June 13, 2007
    Date of Patent: July 6, 2010
    Assignee: XOMA Technology Ltd.
    Inventors: Marc D. Better, Patrick D. Gavit