Abstract: The invention describes catalytic nucleic acid based compounds capable of cleaving nucleic acid polymers both in vivo and in vitro. Two embodiments of this invention are compounds with a short stem that does not base pair, a minizyme, and compounds with DNA hybridizing arms and RNA catalytic domain and stem, DNA-armed ribozymes. The compounds of this invention, while nucleotide based may be substituted or modified in the sugar, phosphate, or base. Methods of use and methods of treatment are also described.

Abstract: The invention provides a method for diagnosing in a subject a neoplastic condition which comprises (a) obtaining from the subject a sample of a biological fluid; and (b) detecting the presence in the sample of a mutant p53 polypeptide encoded by an activated p53 oncogene, the presence of the mutant p53 polypeptide in the sample indicating that the subject has the neoplastic condition.

Abstract: This invention provides an isolated nucleic acid, vectors, transformed mammalian cells and non-human transgenic animals that encode and express normal or mutant .alpha. 1a, .alpha. 1b and .alpha. 1c adrenergic receptor genes. This invention also provides a protein, and an antibody directed to the protein and pharmaceutical compounds related to .alpha. 1a, .alpha. 1b and .alpha. 1c adrenergic receptors. This invention provides nucleic acid probes, and antisense oligonucleotides complementary to .alpha. 1a, .alpha. 1b and .alpha. 1c adrenergic receptor genes. This invention further provides methods for determining ligand binding, detecting expression, drug screening, and treatments for alleviating abnormalities associated with human .alpha. 1a, .alpha. 1b and .alpha. 1c adrenergic receptors.

Abstract: This invention provides a method of increasing sensitivity of cells to the cyctotoxic effects of ionizing radiation which includes incubating the cells with a spindle poison in a suitable carrier at a concentration effective to inhibit the cells from progressing through G.sub.2 or M phases of the cell cycle for a time effective to inhibit division of the cells and then administering a cyctotoxic dose of ionizing radiation to the cells.

Abstract: This invention provides a method for producing a temporally spaced subtracted cDNA library comprising: a) isolating temporally spaced RNAs from cells; b) generating cDNA inserts from the RNAs isolated from step (a); c) producing a temporally spaced cDNA library having clones containing the cDNA inserts generated from step (b); d) producing double stranded cDNA inserts from the temporally spaced cDNA library; e) denaturing the double stranded cDNA inserts; f) contacting the denatured double stranded cDNA inserts produced in step (e) with single-stranded DNAs from another cDNA library under conditions permitting hybridization of the single-stranded DNAs and the double-stranded cDNA inserts; g) separating the hybridized cDNA inserts from the unhybridized inserts; h) generating a cDNA library of the unhybridized inserts, thereby generating a temporally spaced subtracted cDNA library. The cDNAs are then used to monitor cellular differention.

Abstract: A composition for the upregulation of expression of cell antigens, without inducing shedding, which comprises a protein kinase C activator is provided by this invention. Further provided by this invention is a method of detecting and treating tumor cells comprising contacting tumor cells with an effective amount of a protein kinase C activator for the upregulation of expression of antigens of tumor cells, without inducing antigen shedding, and detecting the presence of said antigen or then further contacting said tumor cells with an effective amount of an antibody directed to said antigen.

Abstract: This invention provides an isolated, vertebrate nucleic acid molecule encoding the normal protein that prevents development of Wilson's disease. This invention also provides a nucleic acid molecule comprising a nucleic acid molecule of at least 15 nucleotides capable of specifically hybridizing with a sequence included within the sequence of the above-described nucleic acid molecule. Finally, this invention provides various uses of the isolated Wilson's disease gene.

Abstract: A gas turbine moving blade, which has a blade root section, a wing section, and a platform section, comprises a refrigerant supply port provided in the blade root section, a refrigerant recovery port provided in the blade root section, a serpentine passage provided in the wing section and communicating with the refrigerant supply port and the refrigerant recovery port, and a convection-cooling passage provided in the platform section and allowing sealing air and a refrigerant for cooling the platform section by convection to pass therein.

Abstract: This invention provides a method of detecting metastatic thyroid cancer in a subject which comprises detecting circulating thyroid cells in a bodily fluid sample of the subject by obtaining an appropriate nucleic acid sample from the bodily fluid sample of the subject; and determining whether the nucleic acid sample contains a marker sequence. Specifically, this invention provides wherein the marker sequence is mRNA corresponding to the reverse transcript of DNA encoding thyroglobulin. Also, this invention provides wherein the marker sequence is mRNA corresponding to the reverse transcript of DNA encodes thyroid peroxidase. This invention further provides a test kit for performing the above-described method.

Abstract: This invention provides a method of diagnosing periodontal disease in a subject by detecting elevated concentrations of .beta.-glucuronidase in saliva from the subject. The concentration of .beta.-glucuronidase in the subject's saliva may be determined by adding to a sample of the saliva a substrate for .beta.-glucuronidase and measuring the amount of a product produced by the reaction of .beta.-glucuronidase on the substrate. Also, the concentration of .beta.-glucuronidase in the subject's saliva may be determined by adding to a sample of saliva a labeled antibody specific for .beta.-glucuronidase and measuring the amount of labeled antibody which complexes with .beta.-glucuronidase present in the saliva.

Abstract: Method for fabricating an inner shield for use in a color cathode ray tube, is disclosed, for preventing degradation of a color finesse of the color cathode ray tube, the method including the steps of drawing or forming a piece of thin sheet of aluminum killed steel to form an inner shield of required form, annealing the inner shield in a reducing atmosphere at a temperature above a recrystallization temperature of the aluminum killed steel for improving a non-uniform magnetic distribution thereof, and coating black ironoxide film on a surface of the annealed inner shield.

Abstract: A cleaning method for a flame detector of a gas turbine activating combustor, comprising branching control air for forcing a spark plug of an ignition system into the gas turbine activating combustor, and blowing the branched control air against the respective inner surfaces of light sensor glasses of a flame detector, thereby blowing off dust or soot from the glass.

Abstract: An improved vector upon introduction into a suitable bacterial host containing the thermolabile repressor C.sub.I renders the host cell capable, upon increasing the temperature of the host cell to a temperature at which the repressor is destroyed, of effecting expression of a desired gene inserted into the vector and production of polypeptide encoded by the gene. The vector is a double-stranded DNA molecule which includes in 5' to 3' order the following: a DNA sequence which contains the promoter and operator P.sub.L O.sub.

Abstract: A genetic construct for use in production of transgenic plants with reduced susceptibility or increased resistance to pests or diseases comprises an isolated nucleotide sequence encoding the glucose oxidase enzyme of Talaromyces flavus, the nucleotide sequence being operably linked to a promoter capable of expression in a plant, plant cell or group of plant cells, and further comprises a nucleic acid segment having a nucleotide sequence encoding a signal sequence which directs secretion of the functional glucose oxidase enzyme of T. flavus from plant cells.

Abstract: This invention provides a method of generating a subtracted cDNA library of a cell comprising: a) generating a cDNA library of the cell; b) isolating double-stranded DNAs from the cDNA library; c) releasing the double-stranded cDNA inserts from the double-stranded DNAs; d) denaturing the isolated double-stranded cDNA inserts; e) hybridizing the denatured double-stranded cDNA inserts with a labelled single-stranded nucleic acid molecules which are to be subtracted from the cDNA library; and f) separating the hybridized labeled single-stranded nucleic acid molecule from the double-stranded cDNA inserts, thereby generating a subtracted cDNA library of a cell. This invention also provides different uses of the subtracted library.

Abstract: This invention provides an isolated protein of approximately 40 kDa which is purified from human aortic tissue and immunoreactive with AAA-associated immunoglobulin. Also provided are a method of diagnosing AAA disease in a subject using said isolated protein and a pharmaceutical composition comprising said isolated protein. A method of alleviating AAA disease in a subject comprising administering said pharmaceutical composition comprising the isolated protein is also provided. The subject invention also provides a recombinantly produced human aortic protein which is immunoreactive with AAA-associated immunoglobulin. Also provided are a method of diagnosing AAA disease in a subject using said recombinantly produced protein and a pharmaceutical composition comprising said recombinantly produced protein. A method of alleviating AAA disease in a subject comprising administering said pharmaceutical composition comprising the recombinantly produced protein is also provided.

Abstract: A multi-nozzle combustor comprising a pilot nozzle for flame retention and a plurality of main nozzles arranged around the pilot nozzle, each of the nozzles including an outer cylinder, an inner cylinder, and an annular passage defined between the outer and inner cylinders and forming therein a mixture-evaporation region for a fuel ejected and atomized by an airflow.

Abstract: Method for fabricating a compound semiconductor device including the steps of forming an active layer on a substrate, forming a plurality of ohmic electrodes on predetermined regions of the active layer, forming a mask material layer on the entire surface of the active layer inclusive of the ohmic electrodes, removing the mask material layer from predetermined regions thereof to open regions of the active layer between the ohmic electrodes and at least any one of the ohmic electrodes, etching the opened regions of the active layer each to a predetermined depth, and removing the mask material layer and forming a gate electrode on each of the opened regions of the active layer each having been etched to the predetermined depth, whereby the possibility of forming devices having threshold voltages different from one another on the same substrate allows application of the device of the present invention to variety of MMIC.

Abstract: This invention is directed to imidazolones which are selective antagonists for human .alpha..sub.1a receptors. This invention is also related to uses of these compounds for lowering intraocular pressure, inhibiting cholesterol synthesis, relaxing lower urinary tract tissue, the treatment of benign prostatic hyperplasia, impotency, cardiac arrhythmia, sympathetic mediated pain, migraine, and for the treatment of any disease where the antagonism of the .alpha..sub.1a receptor may be useful. The invention further provides a pharmaceutical composition comprising a therapeutically effective amount of the above-defined compounds and a pharmaceutically acceptable carrier.

Abstract: An apparatus and a method to treat a disease process in a luminal structure. A guidewire is inserted into the patient's luminal structure at least as far as a target area of the luminal structure, a balloon catheter with a blind lumen sealed at its distal end and a guidewire lumen extending therethrough to accept the guidewire is inserted into the luminal structure, the blind lumen being adapted to accept a radiation delivery wire into its proximal end.