Abstract: The present invention relates to the preparation of a novel heat-labile phosphatase enzyme from the filamentous fungus Aspergillus niger. This A. niger phosphatase enzyme has a native molecular weight of approximately 80,000 daltons, and is shown by polyacrylamide gel electrophoresis under denaturing conditions to be an alpha-2 dimer consisting of identical subunits of molecular weight of approximately 37,000 daltons each. The native intact enzyme molecule has an isoelectric point (pI) of 4.6, and exhibits optimal functional activity under reaction conditions of neutral to slightly alkaline pH conditions (about pH 7.0 to about pH 8.5). This enzyme has two characteristics which make it valuable in molecular biology laboratory protocols. First, the enzyme is readily inactivated by mild heating conditions (50.degree. C.); and second, the enzyme is highly specific for DNA as a substrate for the hydrolysis reaction; it does not hydrolyze adenosine triphosphate (ATP).

Abstract: The present invention relates to the preparation of a novel heat-labile phosphatase enzyme from the filamentous fungus Aspergillus niger. This A. Niger phosphatase enzyme has a native molecular weight of approximately 80,000 daltons, and is shown by polyacrylamide gel electrophoresis under denaturing conditions to be an alpha-2 dimer consisting of identical subunits of molecular weight of approximately 37,000 daltons each. The native intact enzyme molecule has an isoelectric point (pI) of 4.6, and exhibits optimal functional activity under reaction conditions of neutral to slightly alkaline pH conditions (about pH 7.0 to about pH 8.5). This enzyme has two characteristics which make it valuable in molecular biology laboratory protocols. First, the enzyme is readily inactivated by mild heating conditions (50.degree. C.); and second, the enzyme is highly specific for DNA as a substrate for the hydrolysis reaction; it does not hydrolyze adenosine triphosphate (ATP).

Abstract: An article having an opaque, pigmented, radiation cured coating is formed by:(a) applying to a substrate a thin layer of pigmented radiation curable material and partially curing same with ionizing irradiation or ultraviolet light in an oxygen containing atmosphere; and(b) then applying a second layer of unpigmented radiation curable material over the partially cured first layer and completely curing both the first and second layers with ionizing irradiation or ultraviolet light in an inert atmosphere.

Abstract: Organic carboxylic acid containing from 2 to 12 carbon atoms and having at least two carboxyl groups or at least one carboxyl group and one amino or hydroxy group or anhydride or halide of such acid is used in making aqueous, foam-growth-controlling printing ink composition for use in chemical embossing of heat-foamable and expandable resinous materials such as polyvinyl chloride.

Abstract: A resin for coating sand and a resin-coated sand produced therefrom wherein the resin is prepared by incorporating furan into a phenolic novolak resin. In the preferred practice, the resin is produced by reaction of furfuryl alcohol with a phenolic compound at an acid pH to give a furfuryl-phenolic intermediate in a first step, and then, in a second step, reacting the furfuryl-phenolic intermediate with a lower aliphatic aldehyde to produce the resin. The resin-coated sands of the present invention provide unexpectedly improved shakeout and collapsibility characteristics in the shell process.

Abstract: A process for production of dextrose from starch wherein a starch hydrolysate having a high dextrose equivalent produced using soluble glucoamylase is treated solely with an immobilized glucoamylase enzyme to produce a dextrose product.

Abstract: Covers a process for producing an improved substantially non-birefringent granular starch product comprising: heating an aqueous slurry of a granular birefringent starch to substantially eliminate the birefringence of the starch and to slightly swell the starch while retaining the granule structure; separating and dehydrating the partially swollen non-birefringent starch granules; and recovering the substantially non-birefringent granular starch product. Also covers the use of this product as a thickener to increase the viscosity and to improve the coating characteristics of latex compositions and said latex compositions.

Abstract: This invention discloses latex compositions wherein a specially treated, substantially non-birefringent, cold water swelling, granular starch product is used as a thickener and for partial replacement for the polymer in the latex.

Abstract: A process for steeping grain wherein the steepwater is inoculated with an inoculum containing a microorganism of the genus Lactobacillus at or near the beginning of the steeping process. The process reduces the steeping time and increases the content of lactic acid in the steepwater. The process also reduces the amount of sulfur dioxide needed in the steeping process.

Abstract: A resin blend useful in molding compounds wherein a novolak resin, a carbohydrate and an aminoplast are blended with fillers and the like to form a molding composition curable with cross linking agents. Articles molded in the molding composition of the invention have good flow characteristics, physical and mechanical properties, and provide molded articles with uniform, smooth surfaces which can be pigmented with bright colors.

Abstract: A carbohydrate-phenolic resol resin and a process for production of same wherein an aldose saccharide, preferably a hexose, is reacted with a phenolic compound and urea in the presence of an acid catalyst to form a liquid fusible resin which is reacted with a lower aliphatic aldehyde in the presence of a basic catalyst to form said resol resin.

Abstract: Stable, homogeneous buttered table syrups are obtained by incorporating in the table syrup formulations an emulsion system consisting of at least 0.1% by weight sodium stearoyl-2-lactylate, at least 0.2% by weight sorbitan monostearate, and at least 2.0% by weight butter. The sugar solids content of the table syrup is adjusted to be no less than 65% Brix, its viscosity is no less than about 300 cps and its pH is about 4.0-5.5. Table syrups containing the emulsion system exhibit good clarity and prolonged shelf life stability.

Abstract: Oil-in-water emulsions containing starch carboxylates as the emulsifying agent. The starch carboxylate imparts improved stability to the emulsions and in the case of salad dressings it can be substituted for all or a portion of the egg content without the loss of any desirable properties.

Abstract: A process for the production of carbohydrate-phenolic resins wherein a phenolic compound is reacted with a hexose saccharide in the presence of a Lewis acid catalyst to form a solid fusible resin which is curable with cross linking agents, such as hexamethylenetetramine.

Abstract: A carbohydrate-phenolic resin and a process for production of same wherein an aldose saccharide, preferably a hexose, is reacted with a phenolic compound and urea in the presence of an acid catalyst to form a solid fusible resin which is curable with cross linking agents, such as hexamethylenetetramine. Instead of urea, use can also be made of diamines, such as toluene diamines and alkylene diamines.

Abstract: A dry, granular mix for making a potage instantly, i.e., in 1 minute or less, upon the addition of boiling or hot water. The mix granules have sizes in the range 10 mesh to 60 mesh, and are formed from a uniformly constituted mixture of certain pulverized dry potage ingredients that are coated with a fat component, and with a finely-divided dispersion aid distributed between the fat-coated particles. The fat component consists of an edible oil or fat and an oleophilic surfactant having an HLB value of about 6 or less.

Abstract: 1,3,4,5-Tetrahydropyrrolo[4,3,2-de]isoquinoline and its N-alkyl derivatives are disclosed. The compounds are antidepressant and antihypertensive agents. Methods for their preparation and use are disclosed.

Abstract: There are disclosed herein the 3.beta., 14-dihydroxy-,3.beta.,5,14-trihydroxy-, 3.beta.,12.beta.,14-trihydroxy- and 3.beta.,14,16.beta.-trihydroxy-21,23-epoxy-23-methoxy-24-nor-5.beta.,1 4.beta.-chola-20,22-diene-22-carbonitriles, as well as their corresponding 3-acetates, 3-propionates and 3-butyrates and their 3-digitosides and 3.beta.-cyamarosyl-glucosides as encountered in naturally-occuring starting materials. The compounds possess useful cardiotonic activity. Also included are the corresponding 22-cyano-5.beta.-card-20(22)-enolides, useful as intermediates in the preparation of the compounds of this invention and also as cardiotonic agents.

Abstract: Indole derivatives characterized by having a 1,3,4,9-tetrahydropyrano[3,4-b]indole or 1,3,4,9-tetrahydrothiopyrano[3,4-b]indole nucleus bearing a substituent in position 1, said substituent incorporating an acid, ester or amide function therein, are disclosed. The nucleus is further substituted at position 1 and may be optionally substituted at positions 3, 4, 5, 6, 7, 8, and 9. The derivatives are useful antiinflammatory, analgesic, antibacterial and antifungal agents and methods for their preparation and use are also disclosed.