Abstract: The present invention provides methods and reagents for assessing lipoprotein metabolism. The methods provided are applicable for use on multiple samples in a clinical laboratory, thus obviating the need for sophisticated instrumentation, such as flow cytometry. Selected cell types in a test sample are uniformly labelled with a detectable reporter substance, so that they may later be enumerated. Pre-determined lipoprotein receptors associated with the cells of interest are labelled with a receptor-selective marker, thereby to determine the number of lipoprotein receptors per cell in the test sample. Selected cells of interest are conveniently separated from the test sample by binding thereto a specific binding substance attached to a solid support. The specific binding substance binds specifically with a characteristic determinant of the cell subset of interest. The remainder of the test sample may be washed away or discarded.
Type:
Grant
Filed:
March 6, 1998
Date of Patent:
June 13, 2000
Assignee:
Lehigh University
Inventors:
Sue Ellen Slezak, John H. Abel, Barbara Obrepalska-Bielska, Eugene A. Nau, Kathy L. Gottlund
Abstract: A process is provided for continuously decomposing ozone in the presence of water or water vapor by contacting the ozone containing gas with a zeolite, such as mordenite, dealuminized mordenite, zeolite Y or zeolite 13 X, at a temperature at which water or water vapor is not sorbed by the zeolite, such as 128 to 175.degree. C.