Abstract: Methods of obtaining mutant nucleic acid sequences that demonstrate elevated oxaloacetate ?-decarboxylase activity are provided. Compositions, such as genetically modified microorganisms that comprise such mutant nucleic acid sequences, are described, as are methods to obtain the same.

Abstract: The present disclosure concerns methods and compositions relating to mixed-library parallel gene trait mapping. In particular embodiments, the methods concern quantitative microarray hybridization techniques for genome-wide identification of trait conferring genes. In other embodiments, the compositions concern genetic elements that confer or are associated with a trait. In an exemplary embodiment, the trait is enhanced growth rate. In another exemplary embodiment, genetic elements that confer enhanced bacterial growth rate comprise part or all of the sequences of SEQ ID NO:1, SEQ ID NO:2, SEQ ID NO:3, SEQ ID NO:4 or SEQ ID NO:5. In other embodiments, the genetic elements that confer enhanced bacterial growth rate correspond to the YliF, adrA, yeaP, yddV or ydeH genes of E. coli.

Abstract: A device for displaying indicia is constructed to fit onto a mirror housing of an exterior mirror of a motor vehicle. The device, upon installation, displays a desired indicia facing the front of the vehicle without obstructing the view of the mirrored surface facing the rear of the vehicle. Variations in structure are provided, including connectors to the mirror housing that slidably engage with respect to a base that supports the indicia. Embodiments provide for removal of the device without harm to the mirror housing, and also provide for ready change-out of indicia. Slidably engaging connectors in various embodiments provides for easy adaptation to a range of sizes and shapes of mirror housings. The invention also relates to kits and methods employing embodiments of the device.

Abstract: In the present invention, viruses, plasmids or both are constructed which contain viral DNA, at least one head-to-head ITR junction, and optionally, recombinase recognition sites positioned such that site-specific recombination between recombinase recognition sites in separate plasmids results in generation of infectious viral DNA at high-efficiency in cotransfected host cells that have been engineered to express a site-specific recombinase. Because of the high-efficiency and specificity of the Cre enzyme, suitably engineered plasmids can be readily recombined to produce infectious virus at high-efficiency in cotransfected 293 cells, without, at the same time, producing wild-type adenovirus, with the attendant problems for removal thereof.

Abstract: This invention responds to a long felt need, by providing in one embodiment, a helper virus based on the Ad2 serotype for use in the Cre/loxP system for the generation of Ad vectors deleted of all Ad protein coding sequences. Using this and helper virus based on Ad5, genetically identical hdAd that differ only in the virion protein components, which are derived from the helper virus, were produced. The vectors have identical expression characteristics in vitro, regardless of the serotype, and the sequential use of hdAd of different serotypes allows for successful repeat vector administration in vivo.

Abstract: Cytokines, including muteins thereof, which are biologically inactive in humans but remain immunogenic, are used in pharmaceutical compositions to promote a neutralizing immune response against native cytokines when administered to a subject in need thereof to treat homeostatic disorders and disorders associated with an overproduction of cytokines.

Abstract: Synthetic DNA molecules encoding HIV gag and modifications of HIV gag are provided. The codons of the synthetic molecules are codons preferred by the projected host cell. The synthetic molecules may be used as a polynucleotide vaccine which provides effective immunoprophylaxis against HIV infection through stimulation of neutralizing antibody and cell-mediated immunity.

Abstract: A transportable play system simulating a backpack or bag unfolds to provide a self-adhering fabric play surface whereby children can create themes or stories by attaching self-adhering play pieces onto the fabric play surface. When the play surface in the closed position to simulate a bag or a backpack, the inner and outer surfaces are held together in a such configuration by hook and loop fasteners such as Velcro®, or other fastening means. Self-adhering play pieces are preferably pre-made to create a theme or story. In other embodiments, the user can create individualized play pieces with supplied fabric stock, and in other embodiments, specific game backgrounds can be added to allow playing of a transportable game. In one embodiment, several backpacks or bags can be interconnected to form a large play surface when unfolded into the open position.

Abstract: A lighted cap is described, comprising a cap having a crown and a bill, and a light structure rotatably fixed to a point on the bill. Rotation to a forward-facing direction for the light results in contacting a contact point that closes a circuit, thereby supplying power to the lamp in the rotatable light structure. The light is used for nighttime activities, such as fishing, when light is needed and a light-weight hat is desired.

Abstract: The present invention provides an improved helper-dependent vector system for production of high capacity adenoviral cloning vectors. The invention makes use of the DNA size packaging constraints imposed on a pIX-defective Ad virion that prevent such virions from packaging DNA larger than approximately 35 kb. This constraint can be used to develop helper viruses that do not package their DNA. In one embodiment, the invention combines this methodology with the Cre-loxP helper-dependent system to decrease the quantity of contaminating helper virus in vector preparations. In another embodiment the invention is used for vector growth.

Abstract: The present invention provides a solution to the problems with known cements by providing a composition comprising (a) microscopic anhydrous pellets or particles containing the most important components of biological fluids or synthetic biological fluids (SBFs), (b) bioactive glass or other bioactive ceramic particles and (c) an appropriate resin such as, but not limited to, bisphenol-alpha-glycidyl methacrylate (BIS-GMA), to form a bone-forming cement which enhances (accelerates) bone production and bone bonding.

Abstract: A device for displaying indicia is constructed to fit onto a mirror housing of an exterior mirror of a motor vehicle. The device, upon installation, displays a desired indicia facing the front of the vehicle without obstructing the view of the mirrored surface facing the rear of the vehicle. Variations in structure are provided. Preferred embodiments provide for removal of the device without harm to the mirror housing, and also provide for ready change-out of indicia. The invention also relates to kits and methods employing embodiments of the device.

Abstract: Devices, methods of using the devices, and kits are described that provide a protective barrier device between an air delivery structure of an air conditioning unit and the ceiling components that are adjacent to the air delivery structure. The device is a unitary water-impermeable structure having surfaces that act as barrier, preventing condensation collecting on the air delivery structure from passing to the sorrounding ceiling components. A preferred embodiment of the invention has a surface projecting into the space being cooled, said surface deflecting air from the adjacent ceiling structures, thereby reducing or preventing collection of dirt or other particles, or moisture, from depositing on the adjacent ceiling components.