Abstract: The invention describes an assay for detecting amplified target nucleic acid sequences with a visual signal. The sensitivity and specificity of the methodology are based on bifunctional target labeling during the amplification step or subsequent hybridization that generates a bifunctional label. The invention may be used, for example, in the screening of amplicon detection for the purpose of more efficiently screening libraries. The invention is also useful to detect nucleic acid sequences indicative of a genetic defect or contagious disease when used with the appropriate primers, as well as detect the existence of nucleic acid amplification.

Abstract: An improved quilt comprising a first, second and third layer of material or fill providing a quilt having superior insulating capabilities and, thus, providing superior warmth to the user. These layers are comprised of at least one layer of fur or simulated fur disposed directly adjacent to a fill layer. Further, the quilt generally, but not necessarily, has a repeating pattern on the third layer of material disposed directly adjacent to the other side of said fill layer. The invention may be used, for example, as bedwear or throw as well as a lap robe.