Abstract: Image acquisition and analysis systems and methods are provided. A ray-based approach may be used to process images for cell-based assays. Such cell-based assays may be used to evaluate drugs or other compounds or to perform other biological studies. A scanning laser microscope or other equipment may be used to gather image data from fluorescently-marked cells or other suitable specimens. The rays are radially-oriented with respect to the cell nuclei. Seed points within the nuclei may be identified. The rays may extend outward from the seed points or other suitable ray origins until the rays are terminated according to ray termination criteria. The intensity of the image data that is associated with each of the rays may be analyzed to generate various parameters. For example, a peak intensity of the image data along each ray may be identified. Statistical calculations may be performed.

Abstract: A liquid ring pump system includes structure configured to automatically and actively vary the amount of seal liquid injected into the sweep of the pump (in order to boost gas discharge pressure) based on one or more variable operating parameters of the pump.

Abstract: A linear pulse-width modulator system is provided. The pulse-width modulation system of the present invention provides a pulse-width modulated (PWM) signal from a control voltage. The PWM signal varies linearly with the control voltage over a full range of duty cycles. The pulse width modulation system of the present invention has as plurality of comparators, each having one input coupled to a control voltage and a second input coupled to a periodic waveform signal provide by a waveform generator. The periodic waveform signals are identical except that each waveform is time delayed with respect to the other waveform signals. The outputs the comparators are coupled to a multiplexer which selects the output of each comparator as the PWM signal for a time interval corresponding to when the output signal of the comparator has substantially constant propagation delays.

Abstract: Methods and apparatus for screening large numbers of chemical compounds and performing a wide variety of fluorescent assays, including live cell assays. The methods utilize a laser linescan confocal microscope with high speed, high resolution and multi-wavelength capabilities and real time data-processing. Imaging may be done at video-rates and with use of ultraviolet illumination.

Abstract: Methods and apparatus for screening large numbers of chemical compounds and performing a wide variety of fluorescent assays, including live cell assays. The methods utilize a laser linescan confocal microscope with high speed, high resolution and multi-wavelength capabilities and real time data-processing. Imaging may be done at video-rates and with use of ultraviolet illumination.