Abstract: The invention relates to a plant-colonizing microorganism which has been genetically engineered to integrate into the chromosome of such microorganism, DNA derived from B. thuringiensis coding for protein toxin. The genetically engineered plant-colonizing microorganisms of the invention, and their progeny, proliferate in commensal or non-detrimental relationship with the plant in the plant environment and are insecticidally active against a subspecies of insect pest which are harmful to the plant. The invention further relates to insecticidal compositions containing such plant-colonizing microorganisms as the active insecticidal agent and to a method of using such genetically engineered plant-colonizing microorganisms in a method of killing or inhibiting insect pests.
Type:
Grant
Filed:
June 7, 1989
Date of Patent:
July 20, 1993
Assignee:
Monsanto Company
Inventors:
Mark G. Obukowicz, Frederick J. Perlak, Lidia S. Watrud
Abstract: Variant somatotropins are described in which the asparagine located in the 95-101 region are replaced by glutamine which somatotropins exhibits enhanced stability.
Type:
Grant
Filed:
August 12, 1991
Date of Patent:
July 14, 1992
Assignee:
Monsanto Company
Inventors:
Gwen G. Krivi, Michael R. Schlittler, Bernard N. Violand
Abstract: Variant forms of mammalian somatotropins, particularly bovine and porcine somatotropin, in which the asparagine located between positions 95-101 has been replaced by glutamine exhibit enhanced stability at high pH and at elevated temperatures while retaining bioactivity equivalent to the corresponding unmutated somatotropins, and are useful in the treatment of animals to enhance growth and/or milk production.
Type:
Grant
Filed:
August 29, 1988
Date of Patent:
February 18, 1992
Assignee:
Monsanto Company
Inventors:
Gwen G. Krivi, Michael R. Schlittler, Bernard N. Violand
Abstract: A method for regenerating soybean plants from cotyledonary nodes is disclosed. Soybean seeds are germinated on nutrient medium containing a cytokinin to produce a donor plant. The cotyledonary nodes of the donor plant are excised and divided into pieces. The cotyledonary node pieces are cultured on nutrient medium containing a cytokinin until callus tissue develops which contains shoots. The shoots are removed and rooted on nutrient medium free of exogenous hormone to form a plantlet.
Abstract: A method of recovering biologically active somatotropins which are produced as insoluble refractile bodies in transformed microorganisms which comprises dissolving refractile bodies in a denaturant and 2-amino-2-methyl-1-propanol, homogenizing, and diluting to fold.
Abstract: This invention involves a cloning or expression vector comprising a gene which encodes 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS) polypeptide which, when expressed in a plant cell contains a chloroplast transit peptide which allows the polypeptide, or an enzymatically active portion thereof, to be transported from the cytoplasm of the plant cell into a chloroplast in the plant cell, and confers a substantial degree of glyphosate resistance upon the plant cell and plants regenerated therefrom.The EPSPS coding sequence may be ligated to a strong promoter, such as the 35S promoter from cauliflower mosaic virus, to create a chimeric gene. Such genes can be inserted into plant transformation vectors, and subsequently introduced into plant cells. Plant cells transformed using such genes and plants regenerated therefrom have been shown to exhibit a substantial degree of glyphosate resistance.
Type:
Grant
Filed:
July 7, 1986
Date of Patent:
July 10, 1990
Assignee:
Monsanto Company
Inventors:
Dilip M. Shah, Stephen G. Rogers, Robert B. Horsch, Robert T. Fraley
Abstract: Synthetic polypeptides are disclosed which exhibit potent serine protease inhibition. Methods and compositions useful for treating conditions caused by unwanted serine protease activity are also disclosed.
Type:
Grant
Filed:
May 8, 1987
Date of Patent:
May 9, 1989
Assignee:
Monsanto Company
Inventors:
George I. Glover, Charles A. McWherter, Charles S. Schasteen
Abstract: Mixtures of an acetanilide derivative and a triazole derivative are found to have surprising efficacy in suppressing seed head development in turf grasses.
Abstract: A process is described for the preparation of thermoplastic elastomers comprising a blend of plastic and cured rubber by dynamic vulcanization wherein the blend is subjected to high shear.
Abstract: Urethane elastomer is described having methylol phenolic groups grafted thereto which modified elastomer is useful for preparing improved polymer blends.
Abstract: A catalyst containing tungsten, an alkali or alkaline earth component, and iron on a support, preferably silica gel, is disclosed which is useful in reverse disproportionation of stilbene and ethylene to produce styrene.
Abstract: A catalyst containing tungsten, potassium, and bismuth on a support, preferably silica gel, is disclosed which is useful in reverse disproportionation of stilbene and ethylene to produce styrene.
Abstract: A catalyst containing tungsten, iridium, and an alkali or alkaline earth component, preferably potassium, on a support, preferably silica gel, is disclosed which is useful in reverse disproportionation of stilbene and ethylene to produce styrene.
Abstract: Urethane elastomer is described having methylol phenolic groups grafted thereto which modified elastomer is useful for preparing improved polymer blends.
Abstract: A catalyst containing tungsten, copper, and an alkali or alkaline earth component on a support, preferably silica gel, is disclosed which is useful in reverse disproportionation of stilbene and ethylene to produce styrene.
Abstract: A process is described for preparing elastoplastic composition which comprises dynamically vulcanizing rubber with a thermoplastic blend of cured rubber and plastic.
Abstract: Alkyl thiosalicylate prevulcanization inhibitors having a pleasant odor are described.Claimed compounds include those of the formula: ##STR1## in which X is an amide inhibitor moiety attached through the nitrogen atom derived from the group consisting of 2-benzimidazolinone, 2-imidazolidinone, 2-benzothiazolone, 2-thiazolone, phthalimide, succinimide, glutarimide, hexahydrophthalimide, maleimide, hydantoin, urea, napthalimide, oxamide, oxanilide, phenylcarbamic acid ester, formamide, formanilide, acetamide, benzamide, acetanilide, benzanilide, propionamide, butyramide, pivalamide, valeramide and hexanamide, R is alkyl of 1-8 carbon atoms, R' is alkyl of 1-8 carbon atoms, alkoxy of 1-8 carbon atoms, --CO.sub.2 --R, or halo, and n is 0, 1 or 2.
Abstract: Vulcanizable rubber compositions are described which are inhibited from premature vulcanization by thio-substituted-1,3,5-triazine-diamine or -triamine.