Patents Represented by Attorney Life Technologies Corporation
-
Patent number: 8329470Abstract: The invention relates to systems and methods for marketing and using products such as liquid materials, especially liquid reagents for use in microbiological and cellular biological laboratory settings include the use of unique color and simple numeric or alphanumeric identifiers to quickly and easily identify any product from a catalog list of products. Methods of marketing, advertising and producing such products are also disclosed. Particular embodiments include products, product packaging and product labeling. The invention also relates to collars and sleeves for containers, as well as related methods of use.Type: GrantFiled: August 1, 2006Date of Patent: December 11, 2012Assignee: Life Technologies CorporationInventors: Axel Unger, Elgin Meike Töpfer, Nicole Busch, Tom Hynek, Fredrik Johan Aidehag, Todd Pelman, Martin Naley
-
Preparation of stable, bright luminescent nanoparticles having compositionally engineered properties
Patent number: 8287761Abstract: A method is provided for preparing luminescent semiconductor nanoparticles composed of a first component X, a second component A, and a third component B, wherein X, A, and B are different, by combining B with X and A in an amount such that the molar ratio B:(A+B) is in the range of approximately 0.001 to 0.20 and the molar ratio X:(A+B) is in the range of approximately 0.5:1.0 to 2:1. The characteristics of the thus-prepared nanoparticles can be substantially similar to those of nanoparticles containing only X and B while maintaining many useful properties characteristic of nanoparticles containing only X and A. The nanoparticles so prepared can additionally exhibit emergent properties such as a peak emission energy less than that characteristic of a particle composed of XA or XB alone; this method is particularly applicable to the preparation of stable, bright nanoparticles that emit in the red to infrared regions of the electromagnetic spectrum.Type: GrantFiled: June 15, 2009Date of Patent: October 16, 2012Assignee: Life Technologies CorporationInventors: Marc Schrier, Donald Zehnder, Joseph Treadway, Joseph Bartel -
Patent number: 8290714Abstract: The use of nanostructures to monitor or modulate changes in cellular membrane potentials is disclosed. Nanoparticles having phospholipid coatings were found to display improved responses relative to nanoparticles having other coatings that do not promote localization or attraction to membranes.Type: GrantFiled: December 31, 2009Date of Patent: October 16, 2012Assignee: Life Technologies CorporationInventors: Michael Ignatius, Elena Molokanova, Alexei Savtchenko
-
Patent number: 8241765Abstract: A fluorescent ink composition comprising functionalized fluorescent nanocrystals, an aqueous-based ink carrier comprising water or a water-based solution, and a binder. Also provided are methods of providing an image or a security mark on a substrate for subsequent identification by applying a fluorescent ink composition onto the substrate.Type: GrantFiled: July 27, 2010Date of Patent: August 14, 2012Assignee: Life Technologies CorporationInventor: Emilio Barbera-Guillem
-
Patent number: 8217433Abstract: To reduce the pixel size to the smallest dimensions and simplest form of operation, a pixel may be formed by using only one ion sensitive field-effect transistor (ISFET). This one-transistor, or 1T, pixel can provide gain by converting the drain current to voltage in the column. Configurable pixels can be created to allow both common source read out as well as source follower read out. A plurality of the 1T pixels may form an array, having a number of rows and a number of columns and a column readout circuit in each column.Type: GrantFiled: March 15, 2012Date of Patent: July 10, 2012Assignee: Life Technologies CorporationInventor: Keith Fife
-
Patent number: 8198084Abstract: The present invention provides a cell culture medium formulation that supports the in vitro cultivation, particularly in suspension, of mammalian cells, particularly epithelial cells and fibroblast cells, and methods for cultivating mammalian cells in suspension in vitro using these media. The media comprise a basal medium and a polyanionic or polyanionic compound, preferably a polysulfonated or polysulfated compound, and more preferably dextran sulfate. The present invention also provides chemically defined, protein-free eukaryotic cell culture media comprising an iron chelate and zinc, which is capable of supporting the growth (and particularly the high-density growth of mammalian cells) in suspension culture, increasing the level of expression of recombinant protein in cultured cells, and/or increasing virus production in cultured cells.Type: GrantFiled: June 14, 2005Date of Patent: June 12, 2012Assignee: Life Technologies CorporationInventors: Stephen F. Gorfien, Richard M. Fike, Glenn P. Godwin, Joyce L. Dzimian, David A. Epstein, Dale Gruber, Don McClure, Paul J. Price
-
Patent number: 8192998Abstract: System for detection and/or analysis of nucleic acids using nanowires to detect covalent modification of nucleic acids.Type: GrantFiled: May 19, 2009Date of Patent: June 5, 2012Inventors: Hongye Sun, Steven Fung, Sam Lee Woo
-
Patent number: 8163562Abstract: The present invention provides a method for reducing undesirable light emission from a sample using at least one photon producing agent and at least one photon reducing agent (e.g. dye-based photon reducing agents). The present invention further provides a method for reducing undesirable light emission from a sample (e.g., a biochemical or cellular sample) with at least one photon producing agent and at least one collisional quencher. The present invention also provides a method for reducing undesirable light emission from a sample (e.g., a biochemical or cellular sample) with at least one photon producing agent and at least one quencher, such as an electronic quencher. The present invention also provides a system and method of screening test chemicals in fluorescent assays using photon reducing agents. The present invention also provides compositions, pharmaceutical compositions, and kits for practicing these methods.Type: GrantFiled: August 23, 2010Date of Patent: April 24, 2012Assignee: Life Technologies CorporationInventors: Tom Knapp, Gregory Zlokarnik, Paul Negulescu, Roger Tsien, Timothy Rink
-
Patent number: 8158194Abstract: Water-dispersible nanoparticles are prepared by applying a coating of a amphipathic dispersant to the surface of a hydrophobic nanoparticle comprised of a semiconductive or metallic material. The amphipathic dispersant is typically polymeric. Preferred polymeric dispersants are comprised of (1) a hydrophobic backbone with hydrophilic branches, (2) a hydrophilic backbone with hydrophobic branches, or (3) a backbone that may be either hydrophobic or hydrophilic, and substituted with both hydrophilic and hydrophobic branches. Monodisperse populations of water-dispersible nanoparticles are also provided, as are conjugates of the water-dispersible nanoparticles with affinity molecules such as peptides, oligonucleotides, and the like.Type: GrantFiled: November 23, 2009Date of Patent: April 17, 2012Assignee: Life Technologies CorporationInventors: Edward Adams, Marcel Bruchez
-
Patent number: 8153828Abstract: The invention provides a compound, useful as an optical probe or sensor of the activity of at least one cytochrome P450 enzyme, and methods of using the compound to screen candidate drugs, and candidate drugs identified by these methods. The optical probe of the invention is a compound having the generic structure Y-L-Q, wherein Q is a chemical moiety that gives rise to optical properties in its hydroxy or hyrdoxylate, phenol or phenoxide form that are different from the optical properties that arise from its ether form.Type: GrantFiled: July 7, 2009Date of Patent: April 10, 2012Assignee: Life Technologies CorporationInventors: Lewis Makings, Gregor Zlokarnik
-
Patent number: 8039642Abstract: The invention provides a novel class of reactive fluorescent agents that are based on a pyrene sulfonic acid nucleus. The agents are readily incorporated into conjugates with other species by reacting the reactive group with a group of complementary reactivity on the other species of the conjugate. Also provided are methods of using the compounds of the invention to detect and/or quantify an analyte in a sample. In an exemplary embodiment, the invention provides multi-color assays incorporating the compounds of the invention.Type: GrantFiled: October 12, 2007Date of Patent: October 18, 2011Assignee: Life Technologies CorporationInventors: Jixiang Liu, Richard Haugland, Wai-Yee Leung
-
Patent number: 8003767Abstract: Fluorescent, sulfonated 3,7-diamino-[8,9]benzophenoxazine dyes are provided that are especially useful for labelling biopolymers and other substrates. The dye-labelled conjugates can be used in a variety of contexts, including cell surface assays employing intact, live cells and in nucleic acid detection methods. The new dyes are water soluble and can be conjugated to a variety of substrates, such as polynucleotides, nucleosides, nucleotides, peptides, proteins, antibodies, carbohydrates, ligands, particles and surfaces.Type: GrantFiled: June 29, 2007Date of Patent: August 23, 2011Assignee: Applied Biosystems, LLCInventors: Xiongwei Yan, Pau Miau Yuan
-
Patent number: 7989617Abstract: The invention describes crown ether chelators, including crown ethers having the formula:Type: GrantFiled: August 24, 2009Date of Patent: August 2, 2011Assignee: Life Technologies CorporationInventors: Kyle Gee, Vladimir Martin
-
Patent number: 7985340Abstract: A magnetic separator unit for the selective separation of at least one component from a fluid mixture is described. The unit can include at least one mandrel-shaped magnet unit and tubing wrapped around it. Fluid containing magnetisable particles can be passed through the tubing to allow attraction of the particles to the magnet unit.Type: GrantFiled: December 1, 2006Date of Patent: July 26, 2011Assignee: Invitrogen Dynal ASInventors: Hilde Kjersti Almaasbak, Christer Johansson
-
Patent number: 7977057Abstract: The present invention provides methods for determining the presence of immobilized nucleic acid employing unsymmetrical cyanine dyes that are derivatives of thiazole orange, a staining solution and select fluorogenic compounds that are characterized as being essentially non-genotoxic. The methods comprise immobilizing nucleic acid, single or double stranded DNA, RNA or a combination thereof, on a solid or semi solid support, contacting the immobilized nucleic acid with an unsymmetrical cyanine dye compound and then illuminating the immobilized nucleic acid with an appropriate wavelength whereby the presence of the nucleic acid is determined. The cyanine dye compounds are typically present in an aqueous staining solution comprising the dye compound and a tris acetate or tris borate buffer wherein the solution facilitates the contact of the dye compound and the immobilized nucleic acid.Type: GrantFiled: April 30, 2010Date of Patent: July 12, 2011Assignee: Life Technologies CorporationInventors: Matthew Beaudet, Gregory Cox, Stephen Yue
-
Patent number: 7948015Abstract: Methods and apparatus relating to very large scale FET arrays for analyte measurements. ChemFET (e.g., ISFET) arrays may be fabricated using conventional CMOS processing techniques based on improved FET pixel and array designs that increase measurement sensitivity and accuracy, and at the same time facilitate significantly small pixel sizes and dense arrays. Improved array control techniques provide for rapid data acquisition from large and dense arrays. Such arrays may be employed to detect a presence and/or concentration changes of various analyte types in a wide variety of chemical and/or biological processes. In one example, chemFET arrays facilitate DNA sequencing techniques based on monitoring changes in hydrogen ion concentration (pH), changes in other analyte concentration, and/or binding events associated with chemical processes relating to DNA synthesis.Type: GrantFiled: December 14, 2007Date of Patent: May 24, 2011Assignee: Life Technologies CorporationInventors: Jonathan M. Rothberg, Wolfgang Hinz, Kim L. Johnson, James Bustillo
-
Patent number: 7943777Abstract: Chemical compounds having a high selectivity for double stranded DNA over RNA and single stranded DNA are disclosed. The chemical compounds are stains that become fluorescent upon illumination and interaction with double stranded DNA, but exhibit reduced or no fluorescence in the absence of double stranded DNA. The compounds can be used in a variety of biological applications to qualitatively or quantitatively assay DNA, even in the presence of RNA.Type: GrantFiled: October 5, 2009Date of Patent: May 17, 2011Assignee: Life Technologies CorporationInventors: Jolene Bradford, Ching-Ying Cheung, Shih-Jung Huang, Patrick Pinson, Stephen Yue
-
Patent number: 7943752Abstract: Disclosed, among other things, are primers containing certain modified nucleobases in the 3? terminal region of the primers that provide reduced formation of primer-dimers during amplification reactions, and various methods of use thereof.Type: GrantFiled: April 14, 2009Date of Patent: May 17, 2011Assignee: Applied Biosystems, L.L.C.Inventors: Khairuzzaman Bashar Mullah, Zhaochun Ma, Wanli Bi
-
Patent number: D637709Type: GrantFiled: March 12, 2008Date of Patent: May 10, 2011Assignee: Invitrogen Dynal ASInventors: Gaute Brede, Darren Ellis, Tine Borgen Hildisch, Jez Mundy, Lars Nokleby, Erlend Ragnhildstveit, Richard Johan Toften, Kornelija Zgonc
-
Patent number: D638953Type: GrantFiled: November 12, 2009Date of Patent: May 31, 2011Assignee: Invitrogen Dynal ASInventors: Gil Cavada, Morten Egeberg, Jamie Hogan, Espir Kahatt, Lars Nøkleby, Lars Norderhaug, Tommy Rivrud, Kornelija Zgonc