Patents Represented by Attorney, Agent or Law Firm Lisa A. Haile
  • Patent number: 6806048
    Abstract: Disclosed is a process for identifying clones having a specified activity of interest, which process comprises (i) generating one or more expression libraries derived from nucleic acid directly isolated from the environment; and (ii) screening said libraries utilizing a fluorescence activated cell sorter to identify said clones. More particularly, this is a process for identifying clones having a specified activity of interest by (i) generating one or more expression libraries derived from nucleic acid directly or indirectly isolated from the environment; (ii) exposing said libraries to a particular substrate or substrates of interest; and (iii) screening said exposed libraries utilizing a fluorescence activated cell sorter to identify clones which react with the substrate or substrates.
    Type: Grant
    Filed: May 3, 2001
    Date of Patent: October 19, 2004
    Assignee: Diversa Corporation
    Inventors: Jay M. Short, Martin Keller
  • Patent number: 6794127
    Abstract: Provided are methods of screening and identification of bio activities and bioactive molecules of interest using a capillary array system. More specifically, disclosed are methods of using optical detection and capillary array-based techniques for screening libraries and recovering bioactive molecules having a desired activity or a nucleic acid sequence encoding such bioactive molecules.
    Type: Grant
    Filed: October 12, 2000
    Date of Patent: September 21, 2004
    Assignee: Diversa Corporation
    Inventors: William Michael Lafferty, Jay M. Short, Martin Keller
  • Patent number: 6764683
    Abstract: There is disclosed a novel genus of small peptides, much smaller than human TGF&agr;, was discovered as having TGF&agr; biological activity and therefore are useful as pharmacologic agents for the same indications as full length TGF&agr; polypeptide. There is further disclosed that TGF&agr; and consequently the genus of small peptides disclosed herein, was found to have therapeutic activity to stimulate hematopoiesis in patients undergoing cytotoxic cancer chemotherapy and to act as a cytoprotective agent to protect a patient undergoing cancer cytotoxic therapy from gastrointestinal (GI) side effects, such as mucositis and otherwise support the barrier function of the GI tract when it is harmed by cytotoxic therapy.
    Type: Grant
    Filed: December 13, 1999
    Date of Patent: July 20, 2004
    Assignee: Kaleidos Pharma, Inc.
    Inventors: Daniel R. Twardzik, Stefan Paskell, Thomas S. Felker
  • Patent number: 6746867
    Abstract: The invention relates to a family of mammalian genes that are transcribed in the immediate early phase following exposure to Fibroblast Growth Factors (FGF) during mesoderm induction, termed Mesoderm Induction Immediate Early Response (MIER) genes. Defining features of the members of this family include that these genes are a) transcribed in response to fibroblast growth factors (FGF); b) are expressed within 40 minutes of FGF treatment; and c) do not require protein synthesis for transcription. There are at least eleven members within this family. The invention relates generally to compositions of and diagnostic methods relating to the M-MIER gene family, cDNA, nucleotide fragments, polypeptides coded thereby, recombinant host cells and vectors containing M-MIER encoding polynucleotide sequences, recombinant M-MIER polypeptides, and antibodies. By way of example, the invention discloses the cloning and functional expression of different M-MIER polypeptides.
    Type: Grant
    Filed: October 13, 1998
    Date of Patent: June 8, 2004
    Assignee: Genesis Group Inc.
    Inventors: Laura Lee Gillespie, Gary David Paterno
  • Patent number: 6733971
    Abstract: The present invention provides transcription factors associated with the hedgehog signaling pathway that are regulated by dephosphorylation by phosphatases. Hedgehog response elements (HRE) that interact with the dephosphorylated transcription factors are also provided as well as methods for identifying compounds that are phosphatase inhibitors. Methods of treating tumors in a subject by modulating the phosphorylation of the transcription factor are also included.
    Type: Grant
    Filed: August 21, 2001
    Date of Patent: May 11, 2004
    Assignees: Baylor College of Medicine, The Johns Hopkins University School of Medicine
    Inventors: Philip A. Beachy, Ming-Jer Tsai, Sophia Tsai, Venkatesh Krishnan, Chien-Huan Chen
  • Patent number: 6720145
    Abstract: Disclosed is a method for removing polynucleotide from the skin. This polynucleotide can be used to detect dermatitis and distinguish an irritant reaction from an allergic reaction by characterizing the polynucleotide according to the polypeptide which it encodes. Additionally, provided are methods for non-invasive isolation of samples from the skin as well as kits for use in the methods provided herein.
    Type: Grant
    Filed: September 28, 2001
    Date of Patent: April 13, 2004
    Assignee: Dermtech International
    Inventors: Lawrence A. Rheins, Vera B. Morhenn
  • Patent number: 6720175
    Abstract: A method is provided for identifying a compound that modulates a cellular response associated with Homer and mediated by a cell-surface or an intracellular receptor. A method is further provided for identifying a compound that modulates receptor activated calcium mobilization associated with Homer. A method is provided for identifying a compound that inhibits Homer protein activity based on the crystal structure coordinates of Homer protein binding domain. A method is also provided for identifying a compound that affects the formation of cell surface receptors into clusters. Also provided are nucleic acids encoding Homer proteins as well as Homer proteins, and Homer interacting proteins.
    Type: Grant
    Filed: August 18, 1999
    Date of Patent: April 13, 2004
    Assignee: The Johns Hopkins University School of Medicine
    Inventors: Paul F. Worley, Jian Cheng Tu, Bo Xiao, Daniel Leahy, Jutta Beneken, Anthony A. Lanahan, Paul R. Brakeman
  • Patent number: 6716825
    Abstract: The present invention relates to phosphonate compounds, compositions containing them, processes for obtaining them, and their use for treating a variety of medical disorder, e.g., osteoporosis and other disorders of bone metabolisum, cancer, viral infections, and the like.
    Type: Grant
    Filed: November 6, 2002
    Date of Patent: April 6, 2004
    Assignee: The Regents of the University of California
    Inventors: Karl Y. Hostetler, James R. Beadle, Ganesh D. Kini
  • Patent number: 6713302
    Abstract: Growth differentiation factor-6 (GDF-6) is disclosed along with its polynucleotide sequence and amino acid sequence. Also disclosed are diagnostic and therapeutic methods of using the GDF-6 polypeptide and polynucleotide sequences.
    Type: Grant
    Filed: July 18, 2000
    Date of Patent: March 30, 2004
    Assignee: The Johns Hopkins University School of Medicine
    Inventors: Se-Jin Lee, Thanh Huynh
  • Patent number: 6709818
    Abstract: Provided are methods and compositions useful for the diagnosis, prognosis and treatment of hepatic cellular proliferative disorders. The methods include the modulation or analysis of hypemethylated glutathione-S-transferase nuleic acid sequence in hepatic samples and biological fluids.
    Type: Grant
    Filed: October 12, 2000
    Date of Patent: March 23, 2004
    Assignee: The Johns Hopkins University School of Medicine
    Inventors: William G. Nelson, Xiaohui Lin, Julia C. Tchou, Jila Bakker
  • Patent number: 6706509
    Abstract: An isolated polypeptide (JNK) characterized by having a molecular weight of 46 kD as determined by reducing SDS-PAGE, having serine and threonine kinase activity, phosphorylating the c-Jun N-terminal activation domain and polynucleotide sequences and method of detection of JNK are provided herein. JNK phosphorylates c-Jun N-terminal activation domain which affects gene expression from AP-1 sites.
    Type: Grant
    Filed: May 18, 2001
    Date of Patent: March 16, 2004
    Assignee: The Regents of the University of California
    Inventors: Michael Karin, Masahiko Hibi, Anning Lin
  • Patent number: 6706498
    Abstract: The invention describes a method for the isolation of components from samples, particularly large molecular weight DNA from biological samples. The method involves the application of controlled oscillatory mechanical energy to the sample for short periods of time of about 5 to 60 seconds to lyse the sample and release the component(s) from the sample, followed by standard isolation methods. In preferred embodiments, the method includes the use of a spherical particle for applying the mechanical energy.
    Type: Grant
    Filed: May 22, 2001
    Date of Patent: March 16, 2004
    Assignee: Bio101, Inc.
    Inventors: James W. Gautsch, Michael G. Saghbini, David A. Lippman, Richard C. Dana
  • Patent number: 6699482
    Abstract: The present invention presents novel leptospiral membrane lipoproteins, LipL1 and LipL2, associated with pathogenic strains of Leptospira. LipL1 is of about 35 kDa, and LipL2 is of about 41 kDa. Also disclosed are the method for purifying these proteins from Leptospira, their nucleotide and amino acid sequences, the cloning of the genes encoding the proteins and their recombinant proteins, methods for producing antibodies to these proteins, the resulting antibodies. These proteins, their immunogenic fragments, and antibodies against them, are useful for inducing an immune response to pathogenic Leptospira as well as providing a diagnostic target for leptospirosis.
    Type: Grant
    Filed: November 13, 2001
    Date of Patent: March 2, 2004
    Assignee: The Regents of the University of California
    Inventors: David A. Haake, Ellen S. Shang
  • Patent number: 6699660
    Abstract: The present invention provides methods and materials related to immediate early genes. Specifically, the invention provides isolated immediate early gene nucleic acid, cells that contain isolated immediate early gene nucleic acid, substantially pure polypeptides encoded by immediate early gene nucleic acid, and antibodies having specific binding affinity for a polypeptide encoded by immediate early gene nucleic acid. In addition, the invention provides cDNA libraries enriched for immediate early genes cDNAs, isolated nucleic acid derived from such cDNA libraries, and methods for treating conditions related to a deficiency in a neuron's immediate early gene responsiveness to a stimulus.
    Type: Grant
    Filed: February 5, 1999
    Date of Patent: March 2, 2004
    Assignee: The Johns Hopkins University School of Medicine
    Inventors: Paul F. Worley, Anthony Lanahan, Bernhard Goetz, Holger Hiemisch, Rohini Kuner, Sigrid Scheek, Karoly Nikolich, Eugene Zhukovsky
  • Patent number: 6696559
    Abstract: Novel antimicrobial peptides from bovine and murine neutrophils are provided. The peptides, designated bovine granulocyte peptide A (BGP-A) and murine granulocyte peptide A (MGP-A) were purified to homogeneity from peripheral blood granulocytes. The amino acid and nucleotide sequence of BGP-A and MGP-A are also provided. A synthetic version of BGP-A and MGP-A is also provided. The purified BGP-A peptide is shown to have antimicrobial activity indistinguishable from that of natural BGP-A. Synthetic carboxamidated analogs of BGP-A (BGP-A-amide) and MGP-A (MGP-A-amide) are also provided.
    Type: Grant
    Filed: October 19, 1999
    Date of Patent: February 24, 2004
    Assignee: The Regents of the University of California
    Inventor: Michael E. Selsted
  • Patent number: 6696260
    Abstract: The present invention provides receptors for the growth differentiation factor (GDF) family of growth factors and methods of identifying such receptors. Also included are methods of identifying antibodies which bind to the receptors, peptide fragments of the receptor which inhibit GDF binding, GDF receptor-binding agents capable of blocking GDF binding to the receptor. The receptors of the invention allow the identification of antagonists or agonists useful for agricultural and human therapeutic purposes.
    Type: Grant
    Filed: May 5, 2000
    Date of Patent: February 24, 2004
    Assignee: The Johns Hopkins University School of Medicine
    Inventors: Se-Jin Lee, Alexandra McPherron
  • Patent number: 6685945
    Abstract: An antigenic preparation is provided which contains a 31 Kd outer membrane protein from Leptospira which can be used immunologically as a vaccine for leptospirosis caused by this organism.
    Type: Grant
    Filed: August 19, 1997
    Date of Patent: February 3, 2004
    Assignee: The Regents of the University of California
    Inventors: David A. Haake, David R. Blanco, Cheryl I. Champion, Michael A. Lovett, James N. Miller
  • Patent number: 6686458
    Abstract: The present invention features biarsenical molecules. Target sequences that specifically react with the biarsenical molecules are also included. The present invention also features kits that include biarsenical molecules and target sequences. Tetraarsenical molecules are also featured in the invention.
    Type: Grant
    Filed: April 19, 2002
    Date of Patent: February 3, 2004
    Assignee: The Regents of the University of California
    Inventors: Roger Y. Tsien, B. Albert Griffin
  • Patent number: 6682904
    Abstract: Methods for identifying a hyaluronidase 2 (HYAL2) specific inhibitor, which selectively inhibits HYAL2 activity, but does not substantially affect the activity of non-inflammatory hyaluronidases, are provided. Also provided are HYAL2 specific inhibitors obtained using such a method. In addition, methods for ameliorating an inflammatory disorder or vasculitis condition by specifically inhibiting HYAL2 is provided.
    Type: Grant
    Filed: August 15, 2002
    Date of Patent: January 27, 2004
    Assignee: Deliatroph Pharmaceuticals, Inc.
    Inventor: Gregory I. Frost
  • Patent number: 6680372
    Abstract: The present invention provides antibodies that bind to the growth differentiation factor-7.
    Type: Grant
    Filed: October 5, 1999
    Date of Patent: January 20, 2004
    Assignee: The Johns Hopkins University School of Medicine
    Inventors: Se-Jin Lee, Thanh Huynh