Abstract: The invention provides novel polypeptides having phospholipase activity, including, e.g., phospholipase A, B, C and D activity, patatin activity, phosphatidic acid phosphatases (PAP)) and/or lipid acyl hydrolase (LAH) activity, nucleic acids encoding them and antibodies that bind to them. Industrial methods, e.g., oil degumming, and products comprising use of these phospholipases are also provided.
Type:
Grant
Filed:
March 8, 2005
Date of Patent:
July 12, 2011
Assignee:
Verenium Corporation
Inventors:
Svetlana Gramatikova, Geoff Hazlewood, David Lam, Nelson R. Barton, Blake G. Sturgis, Dan E. Robertson, Jincai Li, Joel A. Kreps, Roderick James Fielding, Robert C. Brown, Amit Vasavada, Xuqiu Tan, Adrian Badillo, Wilhelmus P. Van Hoek, Giselle Janssen, Charles Isaac, Mark J. Burk
Abstract: The invention provides a purified, isolated, synthetic or recombinant phytase enzyme (e.g., SEQ ID NO:2) initially derived from Escherichia coli B. The enzyme has a molecular weight of about 47.1 kilodaltons and has phytase activity (SEQ ID NO:2). The enzyme can be produced from native or recombinant host cells and can be used to aid in the digestion of phytate where desired. In particular, the phytase of the present invention can be used in foodstuffs to improve the feeding value of phytate rich ingredients.
Abstract: The invention provides isolated and recombinant phytase enzymes. In one aspect, the phytases are produced by modification of the wild type appA of E. coli. The enzyme can be produced from recombinant host cells. The phytases of the invention can be used to aid in the digestion of phytate where desired. In particular, the phytases of the invention can be used in foodstuffs to improve the feeding value of phytate rich ingredients. The phytases of the invention can be thermotolerant and/or thermostable. Also provided are methods for obtaining a variant polynucleotide encoding a phytase and for obtaining a phytase with thermostability or thermotolerant at high or low temperatures.
Type:
Grant
Filed:
October 31, 2007
Date of Patent:
November 2, 2010
Assignee:
Verenium Corporation
Inventors:
Jay M. Short, Kevin A. Gray, Nelson R. Barton, James B. Garrett, Eileen O'Donoghue, Dan E. Robertson
Abstract: The invention relates to alpha amylases and to polynucleotides encoding the alpha amylases, and methods of making and using them. In addition methods of designing new alpha amylases and methods of use thereof are also provided. The alpha amylases have increased activity and stability at increased pH and temperature.
Type:
Grant
Filed:
April 6, 2006
Date of Patent:
February 23, 2010
Inventors:
Walter Callen, Toby Richardson, Gerhard Frey, Carl Miller, Martin Kazaoka, Jay M. Short, Eric J. Mathur