Abstract: The addition of buffer to a harvest stream during diafiltration can cause increased turbidity and have other undesirable effects including limiting product recovery. Methods and compositions related to the use of a non-ionic surfactant are provided for improving diafiltration.
Abstract: Separation methods, for example, to isolate a recombinant protein, are disclosed. In some implementations, a method includes forming a solid containing a first cation and a first anion in a medium containing a protein, and separating the solid from the protein.
Type:
Grant
Filed:
September 8, 2006
Date of Patent:
December 21, 2010
Assignee:
Wyeth LLC
Inventors:
Jonathan L. Coffman, Russell I. Shpritzer, Steven M. Vicik