Abstract: Embodiments described herein provide methods of performing a process for determining an item of interest in a sample. In one embodiment, a container for holding the sample is accepted in a process lane where a process step is selectively automatically performed on the sample in the container. The process step is selectively automatically performed on the sample in the container. An effective length of the process lane is maintained constant while a physical length of the process lane is selectively varied.

Abstract: Embodiments described herein relate to methods and structures for controlling a valve. One embodiment provides a valve control comprising a first valve fluidly connected with a first fluid conveying conduit and a second fluid conveying conduit. The first valve is movable between a first position where fluid communicates between the first fluid conveying conduit and the second fluid conveying conduit and a second position where fluid does not communicate between the first fluid conveying conduit and the second fluid conveying conduit. A first source of relatively increased pressure and a first source of relatively reduced pressure are provided. A third conduit fluidly connects the first source of relatively increased pressure and the first source of relatively reduced pressure with the first valve. A third valve is fluidly connected with the third conduit.

Abstract: Embodiments described herein relate to methods and structures for controlling a valve. One embodiment provides a valve control comprising a first valve fluidly connected with a first fluid conveying conduit and a second fluid conveying conduit. The first valve is movable between a first position where fluid communicates between the first fluid conveying conduit and the second fluid conveying conduit and a second position where fluid does not communicate between the first fluid conveying conduit and the second fluid conveying conduit. A first source of relatively increased pressure and a first source of relatively reduced pressure are provided. A third conduit fluidly connects the first source of relatively increased pressure and the first source of relatively reduced pressure with the first valve. A third valve is fluidly connected with the third conduit.

Abstract: Embodiments described herein relate to methods and structures for controlling a valve. One embodiment provides a valve control comprising a first valve fluidly connected with a first fluid conveying conduit and a second fluid conveying conduit. The first valve is movable between a first position where fluid communicates between the first fluid conveying conduit and the second fluid conveying conduit and a second position where fluid does not communicate between the first fluid conveying conduit and the second fluid conveying conduit. A first source of relatively increased pressure and a first source of relatively reduced pressure are provided. A third conduit fluidly connects the first source of relatively increased pressure and the first source of relatively reduced pressure with the first valve. A third valve is fluidly connected with the third conduit.

Abstract: Embodiments described herein provide a valve construction. One embodiment comprises a first valve and a second valve. A first fluid conveying conduit fluidly conveying a first fluid is fluidly connected with the first valve. A second fluid conveying conduit fluidly conveying a second fluid is fluidly connected with the second valve. A third fluid conveying conduit is fluidly connected with the first valve and the second valve. The first valve is movable between a first position where first fluid flows between the first fluid conveying conduit and the third fluid conveying conduit, and a second position where first fluid does not flow between the first fluid conveying conduit and the third fluid conveying conduit.

Abstract: Methods of fluid handling are disclosed. According to one method, a tip of a nozzle is moved below a surface of the fluid to be handled. A pump fluidly associated with the nozzle is energized to move fluid into the nozzle. A pressure transducer fluidly associated with the nozzle is energized to monitor pressure within the nozzle. Pressure within the nozzle is substantially continuously monitored with the pressure transducer during operation of the pump. Based on the substantially continuously monitored pressure within the nozzle, it is determined whether movement of fluid into the nozzle is intended or unintended. Similar methods may be used to move fluid out of the nozzle.

Abstract: One embodiment of the invention provides a joining element for joining a tube with a port. The joining element comprises a seal having a bore for accepting the tube, a nut and a ferrule. The ferrule has a bore for accepting the seal and the tube, a retaining member which retains the tube with the ferrule by engaging the tube, and a positive stop which limits engagement of the retaining member and the tube. The nut has a bore for accepting the ferrule and the tube and a bearing surface which engages the positive stop on the ferrule to positively limit engagement of the retaining member and the tube. Still other embodiments provide methods for forming a connection between a tube and a port. One such method comprises inserting the tube into a first member and a second member. The first member is moved with respect to the second member such that the second member is positioned partially within the first member. The first member, the second member and the tube are placed in the port.

Abstract: A device for analyzing a whole blood sample is provided. The device comprises a conventionl hematology analyzer integrated with a fluorescence cyometry analyzer. A controller is provided for controlling the analyzers, obtaining and utilizing data from both and reporting a quantitative result. Methods are also provided for analyzing a whole blood sample. One such method comprises the steps of performing on a single instrument an analysis of impedance associated with the blood sample, an analysis of light scatter associated with the blood sample, and an analysis of fluorescence associated with the blood sample. Data is collected and utilized. A result is reported.

Abstract: A device for analyzing a whole blood sample is provided. The device comprises a conventional hematology analyzer integrated with a fluorescence cytometry analyzer. A controller is provided for controlling the analyzers, obtaining and utilizing data from both and reporting a quantitative result. Methods are also provided for analyzing a whole blood sample. One such method comprises the steps of performing on a single instrument an analysis of impedance associated with the blood sample, an analysis of light scatter associated with the blood sample, and an analysis of fluorescence associated with the blood sample. Data is collected and utilized. A result is reported.

Abstract: A pseudo telecentric flow cytometric optical system for the simultaneous detection of several characteristics of particles suspended in a flowing medium. The system is made up of a flowcell through which the particles pass substantially one particle at a time, an optical system for directing light from a light source onto the flowing particles in the flowcell, a side angle optical collection system for receiving light from the flowing particles and for directing the light to one or more of a first set of detectors, and a forward angle collection system for receiving light from the flowing particles and for directing the light to one or more of a second set of detectors.

Abstract: Embodiments described herein provide methods and apparatuses for thermally controlling an instrument. According to one embodiment, an apparatus includes a heater and a source of electrical energy electrically connected with the heater for energizing the heater to produce thermal energy. A driver is electrically connected with the heater and the source of electrical energy for controlling application of electrical energy to the heater. A sensor is electrically connected with the heater for detecting thermal energy produced by the heater responsive to the electrical energy from the source of electrical energy. A controller is electrically connected with the driver and the sensor for controlling production of thermal energy by the heater responsive to electrical energy from the source of electrical energy. Another embodiment provides a method in which a heater is electrically connected with a relatively reduced thermal energy reference resistor.

Abstract: According to one embodiment, a nozzle for introducing a fluid comprises a first conduit having a first end and a second conduit having a second end. The second conduit is disposed substantially concentrically with the first conduit. The first end is offset from the second end. A third conduit having a third end is provided with the third conduit being disposed substantially concentrically with the first conduit and the second conduit and the third end being offset from the first end and the second end. Another embodiment provides a method wherein a first fluid conveying conduit, a second fluid conveying conduit and a third fluid conveying conduit are provided. The second fluid conveying conduit substantially surrounds the first fluid conveying conduit and the third fluid conveying conduit substantially surrounds both the first fluid conveying conduit and the second fluid conveying conduit. A first fluid flows through the first fluid conveying conduit.

Abstract: Embodiments described herein provide apparatuses and methods for preparing fluid. In one method, a heater thermally associated with a housing for receiving fluid is operated to apply thermal energy to the housing. A second predetermined volume of a second fluid is introduced to the housing. The second predetermined volume of the second fluid is moved with the housing for a first predetermined time period. The first predetermined volume of the first fluid is introduced to the housing. The first predetermined volume of first fluid and the second predetermined volume of second fluid are moved with the housing for a second predetermined time period. The first predetermined volume of first fluid and the second predetermined volume of second fluid are removed from the housing. Another embodiment provides an apparatus comprising a first housing for containing fluid. A second housing is operatively connected with the first housing.

Abstract: Embodiments disclosed herein provide apparatuses and methods for verifying dispense of a fluid from a dispense nozzle. According to one method, a path of electromagnetic radiation from a source to a receiver is obstructed with the fluid dispensed from the dispense nozzle. The intensity of the electromagnetic radiation received by the receiver is measured. The measured intensity is compared with a predetermined intensity to verify the dispense of fluid from the dispense nozzle. One apparatus for verifying dispense of a fluid comprises a source of electromagnetic radiation, and a receiver of the electromagnetic radiation from the source of the electromagnetic radiation operatively associated with the source of the electromagnetic radiation such that the electromagnetic radiation from the source is received by the receiver.

Abstract: A mover for moving a container and a method of moving a container are provided. One container mover comprises a prime mover and a container engaging element operatively connected with the prime mover such that the container engaging element moves responsive to the prime mover to move the container. A driver is operatively connected with the container engaging element for moving the container engaging element between a first position where the container engaging element engages the container and a second position where the container engaging element is offset from the container. In one method of moving a container with a container mover including a container engaging element, the container engaging element is moved such that the container engaging element engages the container. The container engaging element and the container are moved conjointly. The container engaging element is moved such that the container engaging element is offset from the container.

Abstract: Embodiments described herein relate to a valve and a method of use. In one embodiment, a first element is operatively and removably positioned with respect to a first fluid conveying conduit and a second fluid conveying conduit. The first element is movable between a first position where no fluid communicates between the first fluid conveying conduit and the second fluid conveying conduit and a second position where fluid communicates between the first fluid conveying conduit and the second fluid conveying conduit. A second element is operatively and removably engagable with the first element for maintaining operative position of the first element with respect to the first fluid conveying conduit and the second fluid conveying conduit. A third element is operatively and removably engagable with the second element for maintaining engagement between the first element and the second element.

Abstract: A pipetting apparatus is provided with clot detection. The pipetting apparatus comprises a nozzle for aspirating a sample. A pressure sensor is connected with the nozzle for measuring pressure in said nozzle. A plurality of pressure difference calculating circuits are operatively connected with the pressure sensor, each for inputting an output of said pressure sensor and obtaining a pressure difference at a different pressure calculation period, respectively. A plurality of discriminating circuits each having a different discrimination threshold value determined according to each of the pressure calculation periods are provided. An alarm circuit is included for outputting a clot detection alarm signal when at least one of said discriminating circuits discriminates that the obtained pressure difference exceeds the discrimination threshold value.

Abstract: Embodiments described herein provide methods of analysis of a reaction, such as an agglutination reaction producing an agglutination pattern, and the like. In one method, an image of the agglutination pattern is captured. The image captured is digitized to form a digital image comprising a pixel. Roughness is measured, reflecting pixel local environment, at each pixel comprising a region of interest of the digital image of the agglutination pattern. At least one of classification and quantification of the image captured of the agglutination pattern is performed based on the measured roughness. In another embodiment, roughness is derived using a shared weights neural network approach, the roughness reflecting pixel local environment at each pixel comprising a region of interest of the digital image of the agglutination pattern. At least one of classification and quantification of the image captured of the agglutination pattern is performed based on the derived roughness.

Abstract: Embodiments described herein provide a pipetting apparatus with leak detection and a method for detecting a leak. In one embodiment, an automatic pipetting apparatus comprises a nozzle having a lower end portion through which a fluid sample is aspirated and dispensed by changes in air pressure supplied to the nozzle. A piping system operatively connects the nozzle to a pump for supplying the air pressure to the nozzle. A pressure sensor is operatively connected with the piping system for measuring internal pressure in the piping system. An XYZ robot is operatively connected with the nozzle for three-dimensionally moving the nozzle between an aspirating position of the fluid sample and a dispensing position.