Abstract: This invention involves the elution of macromolecules such as proteins, deoxyribonucleic acid(DNA), ribonucleic acid(RNA) from solid phase matrix such as polyacrylamide, agarose and membranes such as polyvinyl difluoride (PVDF) in an electric field. The elution chamber shown in FIG. 1 consists of two separate screws 9, 9a and attachable chamber 1. The screw well 8 holds the gel matrix containing the macromolecules and is separated by the bottom chamber 1 with a membrane 5 with a nominal molecular weight cutoff higher than the macromolecule. On the other hand, the macromolecule is retained in the well 1 by introducing another membrane 6 having a molecular weight cutoff lower than the molecular weight of the macromolecule. Alternatively, macromolecules separated on membrane, e.g. PVDF, are held in a Teflon or plastic membrane holder 11. This holder can fit inside well 8 in screw 9. In addition, chamber 1 contains a well 7.

Abstract: A device for the efficient automatic controlling of the speed of a vehicle through a brake-pedal interface via an electronic switch to a vehicle's cruise control system, and the method for operating that device. The switch connects to the vehicle's brake pedal whereby successive taps of the pedal signal and control the cruise control through the switch.