Abstract: The present invention includes a biopsy needle for use in a biopsy gun or as surgical instrument, capable of taking multiple samples, and a guiding needle holder for holding a biopsy needle. The biopsy needle includes an inner needle, also called a stylet, and an outer needle, also called a cannula, and a guiding needle holder. The stylet has a recess in the vicinity of its distal end which receives the tissue sample. The guiding needle holder includes an integrated spacer, and first and second connecting elements. The first connecting element holds the proximal end of the cannula and is displaceably attached along the length of the integrated spacer. The second connecting element holds the proximal end of the stylet and is fixedly attached to the distal end of the integrated spacer. The connecting elements each have an opening that facilitates insertion of the biopsy needle into a known biopsy gun in either the cocked or uncocked position.