Abstract: The invention relates to a process for the culturing of cells, preferably E1-immortalized HER cells, more preferably PER.C6 cells in a reactor in suspension in a cell culture medium, wherein the cells produce a biological substance, preferably an antibody, wherein at least one cell culture medium component is fed to the cell culture and wherein the cell culture comprising the cells, the biological substance and cell culture medium is circulated over a separation system and wherein the separation system separates the biological substance from substances having a lower molecular weight than the biological substance and wherein the biological substance is retained in or fed back into the reactor. Preferably part of the substances of lower molecular weight is continuously removed from the cell culture.

Abstract: A method of scoring Oestrogen and Progesterone Receptors expression (ER and PR) from histological images determines the percentage of brown image blob area in total blob area and derives percentage thresholds to quantify scoring. Brown blob area proportion is then compared with the thresholds to provide a first contribution to scoring of ER or PR. The number of relatively dark pixels is then counted, pixel number thresholds are determined to quantify scoring, and the number of relatively dark pixels is compared with the thresholds to provide a second contribution scoring of ER or PR. The two contributions are then added to provide an overall scoring in the range 0 to 8 which may be taken as a final score or it may be scaled to a conventional range 0 to 3.

Abstract: Structures and methods are disclosed to produce mechanical strength in Micro Electro Mechanical Systems by increasing the moment of inertia of some of the composing elements. In one aspect, a thermal sensor with improved mechanical strength, thermal insulation and time constant is achieved. Moreover, the current method and apparatus is advantageous in terms of process time and process cost, particularly in the area of lithographic patterning.

Abstract: Methods and apparatuses are provided for modeling the compensation error and simulating registration error of a multilayer printed circuit board. Experiments are conducted in order to model compensation error which factor one or a combination of the following: the dielectric layer, the position of the core in the stack, the circuit configuration, the assembly of the printed circuit board, and the interaction between the core and the dielectric layer. The registration simulator combines the sources of registration error in an interdependent manner so as to model overall registration error over the panel surface.