Abstract: The present disclosure provides methods for obtaining the targeted integration of a DNA molecule into the genome of a host cell using a recombinase. The methods disclosed herein can be used with a variety of host cells, including, for example, dicotyledonous and monocotyledonous plant cells. The present disclosure provides a method for effecting site-specific recombination of DNA within a plant cell, comprising: introducing into the plant cell a target nucleotide sequence comprising a first Int recognition site; introducing into the plant cell a donor nucleotide sequence comprising a second Int recognition site; and introducing into the plant cell an integrase or integrase complex.
Type:
Grant
Filed:
March 28, 2003
Date of Patent:
April 1, 2008
Assignee:
Syngenta Participations AG
Inventors:
Janet Louise Suttie, Mary-Dell Chilton, Qiudeng Que
Abstract: Methods for making transgenic plants that are resistant to HPPD herbicides are presented. Polynucleotides other than those from Pseudomonas fluorescens that encode resistant HPPD enzymes are enclosed for use in the process of making transgenic plants that are tolerant to HPPD-inhibiting herbicides.
Type:
Grant
Filed:
November 14, 2001
Date of Patent:
December 25, 2007
Assignee:
Syngenta Limited
Inventors:
Christopher John Andrews, Simon Anthony James Warner, Timothy Robert Hawkes
Abstract: Promoter sequences capable of driving gene expression in plants are presented. The promoters are capable of driving constitutive expression of an associated nucleotide sequence encoding a protein that confers a phenotypic trait. The atRSp41 promoter and promoter fragments are described, and these promoters can be used to create transgenic plants expressing a gene or genes of interest at all times and in most tissues and organs.
Type:
Grant
Filed:
April 2, 2001
Date of Patent:
August 14, 2007
Assignee:
Syngenta Participations AG
Inventors:
Andrea Barta, Sergiy Lopato, Maria Kalyna
Abstract: Method for the inhibition of carbon flow in the glycolytic direction in a cell by increasing the intracellular availability of trehalose-6-phosphate.
Type:
Grant
Filed:
October 9, 2003
Date of Patent:
July 24, 2007
Assignee:
Syngenta Mogen B.V.
Inventors:
Oscar Johannes Maria Goddijn, Jan Pen, Josephus Christianus Maria Smeekens
Abstract: The present invention provides predominantly callus-specific promoters obtainable from Brassica napus plants. According to the present invention there is provided a DNA fragment harbouring a callus specific promoter, said DNA fragment being present in clone pJB1178-21 or clone pJB1178-43, respectively deposited with the Centraal Bureau of Schimmelcultures (Baarn, the Netherlands) on 6 Feb. 2001 under no. CBS 109271 and no. CBS 109273. The DNA fragment according to the present invention is further characterised in that it comprises the nucleotide sequence represented by SEQ ID NO: 1 or SEQ ID NO: 2 or the full length sequences as depicted in SEQ ID NO: 8 or SEQ ID NO: 9 respectively or parts thereof. Further, the invention comprises the homologue sequences in Arabidopsis thaliana as depicted in SEQ ID NO: 10 and SEQ ID NO: 11, respectively.
Type:
Grant
Filed:
May 31, 2002
Date of Patent:
April 3, 2007
Assignee:
Syngenta Limited
Inventors:
Jacob Bernardus Bade, Jerome Hubertina Henricus Victor Custers
Abstract: The Arabidopsis NI16 gene was isolated in a yeast 2-hybrid screen via its interaction with the NIM1 protein and encodes a protein involved in the regulation of SAR gene expression in plants. NI16 is strongly induced in NIM1-overexpressing plants treated with benzo(1,2,3)thiadiazole-7-carbothioic acid S-methyl ester (BTH). The nucleic acid sequence of the Arabidopsis NI16 promoter is disclosed herein.
Type:
Grant
Filed:
March 12, 2004
Date of Patent:
April 3, 2007
Assignee:
Syngenta Participations AG
Inventors:
Rebecca M. Cade, Robert A. Dietrich, Kay Ann Lawton
Abstract: This invention describes novel DNA sequences that function as promoters of transcription of associated nucleotide sequences. More specifically, this invention describes DNA sequences conferring constitutive expression to an associated nucleotide sequence. The invention also describes recombinant sequences containing such promoter sequences. The said recombinant DNA sequences may be used to create transgenic plants, but especially plants expressing a nucleotide sequence of interest at all times and in most tissues and organs.
Type:
Grant
Filed:
March 26, 2001
Date of Patent:
January 23, 2007
Assignee:
Syngenta Participations AG
Inventors:
Thomas Hohn, Livia Stavolone, Petrus Theodorus De Haan, Hope Thompson Ligon, Maria Kononova