Abstract: The present invention provides an integrated, fully automated, high-throughput system for two-dimensional electrophoresis comprised of gel-making machines, gel processing machines, gel compositions and geometries, gel handling systems, sample preparation systems, software and methods. The system is capable of continuous operation at high-throughput to allow construction of large quantitative data sets.

Abstract: The present invention provides an integrated, fully automated, high-throughput system for two-dimensional electrophoresis comprised of gel-making machines, gel processing machines, gel compositions and geometries, gel handling systems, sample preparation systems, software and methods. The system is capable of continuous operation at high-throughput to allow construction of large quantitative data sets.

Abstract: The present invention provides an integrated, fully automated, high-throughput system for two-dimensional electrophoresis comprised of gel-making machines, gel processing machines, gel compositions and geometries, gel handling systems, sample preparation systems, software and methods. The system is capable of continuous operation at high-throughput to allow construction of large quantitative data sets.

Abstract: A process for the determination of the presence or concentration of a polyepitopic antigen in a specimen, comprising contacting the specimen with a first reagent comprising one or more monoclonal antibodies to form a conjugate comprising monoclonal antibodies in the first reagent and an antigen in the specimen having epitopes to which said monoclonal antibodies are immunoreactive; contacting the conjugate so formed with a second reagent comprising one or more monoclonal antibodies to form a conjugate comprising the antigen, the monoclonal antibodies from the first reagent and the monoclonal antibodies from the second reagent, wherein one of the first and second reagents comprises two different monoclonal antibodies and the antibodies in the first reagent and the antibodies in the second reagent comprises three different monoclonal antibodies reactive with different epitopes on said antigen; and detecting the conjugate comprising the antigen and the three different monoclonal antibodies.

Abstract: A cellfree extract of Haemophilus parasuis that exhibits toxic activity is described. The cellfree extract is useful as a vaccine or diagnostic reagent.

Abstract: The invention relates to a steroid compound having the formula (I) comprising a ring E, said ring sharing carbon atoms at position 16 and 17 with the five-membered ring D and being &agr; with respect to said D-ring. In addition, the carbon atom at position 17 is substituted with an oxygen atom-comprising group through a CO bond. The invention also relates to a pharmaceutical composition comprising said steroid compound. The steroid compounds of the present invention are very suitable for use in the prevention or treatment of peri-menopausal or menopausal complaints, more preferably the prevention or treatment of osteoporosis. Furthermore, the steroid compounds of the present invention can be used for contraceptive purposes.

Abstract: The present invention is directed to a transcription based amplification method for the amplification of DNA targets. With the method of the present invention an isothermal transcription based amplification method is provided for the amplification of double stranded DNA.

Abstract: The compound mirtazapine is found to be effective in treating sleep apneas. Optionally, mirtazapine is combined with an SSRI such as fluoxetin.

Abstract: The present invention relates to certain novel piperidine derivatives, to processes for their preparation, to pharmaceutical formulations containing them and to their use in medical therapy, particularly in the treatment of psychotic disorders.

Abstract: A C-11 substituted steroid and a method of use of a C-11 substituted steroid to provide an estrogenic effect to a human or animal.

Abstract: An isothermal transcription based amplification assay for the detection or quantification of chemokine RNA uses primers and probes for sequences within the gene for RANTES, MIP-1&agr; and MIP-1 &bgr;. The quantitative system uses an internal control Q of a mutant version of each gene. Target specific primers and probes are also disclosed.

Abstract: A method and a test kit for genotyping the CCR5 chemokine receptor. CCR5 transcript DNA is amplified using transcription based amplification. The amplification product is detected through the hybridization of appropriately labeled oligonucleotide wild type and mutant probes. In a liquid hybridization based ECL analysis, the mutant probe is a 16mer having the sequence 5′TCCATACATTAAAGAT3′ [SEQ ID NO:9]. The method and kit can be used to predict.

Abstract: This invention relates to the use of a DNA vaccine for the manufacture of a medicament for use in eliciting an immune response against parvovirus in a maternally derived antibody positive animals. The DNA vaccine comprises a plasmid vector and at least one isolated nucleotide sequence encoding a parvovirus immunogenic polypeptide, and transcriptional regulatory sequences operably linked to the isolated nucleotide sequence.

Abstract: The present invention provides in situ amplification methods for the detection of a target nucleic acid molecule. The methods use a molecule termed “molecular tape” to retain the amplified target nucleic acid molecule within a cell upon amplification. The molecular tape, which may be used with a variety of amplification methods, improves the in situ detection of an amplified target molecule.

Abstract: The present invention relates to a method of systemically administering a vaccine comprising live attenuated bacteria of the species Streptococcus equi in order to protect against Streptococcus equi infection.

Abstract: An isothermal transcription based amplification assay for the detection or quantification of chemokine RNA uses primers and probes for sequences within the gene for RANTES, MIP-l.alpha. and MIP-1 .beta.. The quantitative system uses an internal control Q of a mutant version of each gene. Target specific primers and probes are also disclosed.

Abstract: The present invention provides oligonucleotides that can be used as primers to amplify a region of the 16S rRNA of M. pneumoniae. Also provided are probes and kits for detection of amplified RNA and typing of M. pneumoniae strains. The primers, probes, methods and kits are useful for diagnosing M. pneumoniae.

Abstract: A vaccine for immunizing animals against diseases caused by microorganisms producing an osteolytic toxin comprising an immunologically effective amount of a recombinant, immunogenic, detoxified Pasteurella multocida toxin or toxin analog, the vaccine being encoded by a nucleotide sequence coding for a Pasteurella multocida toxin or toxin analog which is inserted in an expression vector capable of replicating in a suitable host microorganism in which the said sequence may be expressed. Optionally, the produced toxin or toxin analog is subjected to posttranscriptional modifications to provide the detoxified toxin or toxin analog. Alternatively, the nucleotide sequence may be modified prior to insertion into the expression vector so as to provide the toxin or toxin analog in a detoxified form.

Abstract: An isothermal transcription based amplification assay for MDC RNA uses primer combinations for sequences within the MDC gene. A quantitative control uses a mutant RNA for comparison.

Abstract: The invention relates to a steroid compound having the formula (I) ##STR1## comprising a ring E, said ring sharing carbon atoms at position 16 and 17 with the five-membered ring D and being .alpha. with respect to said D-ring. In addition, the carbon atom at position 17 is substituted with an oxygen atom-comprising group through a CO bond. The invention also relates to a pharmaceutical composition comprising said steroid compound. The steroid compounds of the present invention are very suitable for use in the prevention or treatment of peri-menopausal or menopausal complaints, more preferably the prevention or treatment of osteoporosis. Furthermore, the steroid compounds of the present invention can be used for contraceptive purposes.