Abstract: Disclosed are novel compounds that are A1 adenosine receptor agonists, useful for treating various disease states, in particular tachycardia and atrial flutter, angina, diseases related to release of nonesterified fatty acids, and myocardial infarction.

Abstract: The present invention provides compounds useful for increasing cellular ATP binding cassette transporter ABCA1 production in mammals, and to methods of using such compounds in the treatment of coronary artery diseases, dyslipidiemias and metabolic syndrome. The invention also relates to methods for the preparation of such compounds, and to pharmaceutical compositions containing them.

Abstract: The present invention provides compounds useful for increasing cellular ATP binding cassette transporter ABCA1 production in mammals, and to methods of using such compounds in the treatment of coronary artery diseases, dyslipidiemias and metabolic syndrome. The invention also relates to methods for the preparation of such compounds, and to pharmaceutical compositions containing them.

Abstract: Precursors for use in the synthesis of polynucleotides and methods of using the precursors in synthesizing polynucleotides are disclosed. The precursors include a heterocyclic base having an exocyclic amine group and a substituted or unsubstituted triaryl methyl protecting group bound to the exocyclic amine group.

Abstract: A method of synthesizing a polynucleotide which can, for example, be used during fabrication of an array. A second nucleoside is coupled to a first nucleoside through a phosphite linkage, with the second nucleoside having a hydroxyl protecting group that is a non-carbonate protecting group. The product of the foregoing step is exposed to a composition which both oxidizes the formed phosphite to a phosphate and deprotects the protected hydroxyl of the coupled nucleoside. The method has particular application to fabricating an addressable array of polynucleotides on a substrate which carries substrate bound moieties each with a hydroxyl group.

Abstract: A method of synthesizing polynucleotides is disclosed. The method involves contacting a first nucleotide with a selected reactive group in the presence of an ionic liquid. The selected reactive group may be on a second nucleotide, a polynucleotide, or on a moiety on an insoluble substrate, for example in an oligonucleotide synthesizer.

Abstract: Devices and methods for contactless heating of a fluid in a chamber using a high frequency alternating potential are described. At least two electrodes are coupled to the chamber such that the alternating potential is coupled to the fluid inside the chamber and heats the fluid. A control loop derives a signal from the alternating potential and uses the signal to control the alternating potential. The devices and methods described determine temperature and/or conductivity of the fluid using the signal.

Abstract: A method of fabricating polynucleotide arrays includes dissolving a nucleotide monomer, oligonucleotide, or polynucleotide in a solvent containing ionic liquid and depositing the resulting solution on an array substrate. The method has particular application to fabrication of an addressable array of polynucleotides on a substrate that carries substrate bound moieties each with a hydroxyl group. The process may be repeated at specific locations on the array to elongate the polynucleotide deposited on the array.

Abstract: A pin-in-capillary apparatus for depositing droplets of fluid onto a surface is described. The apparatus has an off-axis channel for supplying fluid to the capillary. In the capillary the fluid wets the tip of the pin and is delivered to the surface when the pin is extended from the capillary. The capillaries and pins are flexible, allowing spatial reformatting for fluid delivery. Methods of delivering fluid to a surface are also described.

Abstract: A high-throughput laser desorption/ionization (LDI) mass spectrometer has been developed and is described herein. The mass spectrometer employs an ion source that has a plurality of lasers firing in tandem at one or more samples to increase the rate at which ion packets are generated by the ion source.

Abstract: A device for sampling blood from the skin of a patient by puncture. The device includes one or more lancets for lancing the skin and a fluid-conducting plate unit for transporting blood from the lancing wound. The plate unit has one or more channels for conducting fluid to one or more target locations. Preferably there is a first channel for conducting away a first portion of fluid that contains more of a fluid that is emitted initially (initial fluid) from the puncture wound and a second channel for conducting a second portion of fluid that contains less of the initial fluid from the puncture wound. Blood is drawn into the channels by capillary force. The device can be used to lance the skin and obtain a representative sample of blood with relatively simple procedures.

Abstract: A chemiluminescence-based gel assay may be used as an indicator of fermenter health. The assay is performed using a sample of fermentation medium essentially directly from the fermenter (with no need for complicated purification of the sample before performing the assay). Results from the assay may be correlated with the final product purification yield of a given protein/purification protocol system. This may then be used as a predictor of purification yield before actually performing the purification.

Abstract: A sensor for sensing in a gas stream a vapor of a liquid. The sensor includes a micropore and a wet temperature sensor. The micropore has an evaporation end and has a lumen to conduct liquid from a supply of the liquid for evaporation at the evaporation end. The wet temperature sensor has a heat sensitive part in contact with the liquid in the micropore. The heat sensitive part circumscribes the micropore and forms part of the lumen. Heat loss due to evaporation of the liquid when the wet temperature sensor wet with the liquid is placed in the gas stream will result in the temperature sensed by the wet temperature sensor being lower than the non-evaporative temperature of the gas stream. This lowering in temperature can be measured to determine the concentration of the vapor in the gas stream. An example of such a sensor has a thermocouple junction having micropores passing through the thermocouple junction.

Abstract: A miniaturized total analysis system with an in-line NMR detection compartment and an NMR rf microcoil detector is described for use in liquid phase analysis. The device is formed by microfabrication of microstructures in novel support substrates. The NMR detector coil may be fabricated directly in the support body at the point of detection or, alternatively, may be formed as part of a modular structure that is insertable into the device at the point of detection. In addition, an integrated device for sample preparation and NMR detection is provided comprising the miniaturized total analysis system and a miniature magnet configured to accept the miniaturized total analysis system, wherein the device is capable of generating an NMR spectrum. The invention herein is used for the analysis of small and/or macromolecular and/or other solutes in the liquid phase.

Abstract: Peptides that have domains that bind to recombinant factor VIII (rFVIII) are disclosed. A method of rFVIII binding assay using the peptides deduced from a combinatorial library in a filtration plate process is described. A method of using peptides having these available binding domains in an affinity chromatography process to purify factor VIII is also taught.

Abstract: BTL.010 is a novel human serine proteinase inhibitor of the Kunitz family that exhibits greater potency towards neutral serine proteinases, particularly leukocyte elastase-, and proteinase 3, than towards trypsin-like proteinases. BTL.010, or variants thereof, may be employed as therapeutics in diseases such as emphysema, idiopathic pulmonary fibrosis, adult respiratory distress syndrome, cystic fibrosis, rheumatoid arthritis, organ failure, and glomerulonephritis in which uncontrolled proteolysis due to neutral serine proteinase activity results in tissue damage.

Abstract: Human/human hybrid cells were made via fusion of human embryonic kidney cells (293S) and modified Burkitt's lymphoma cells (2B8). The fusion cells are useful as host cells for the recombinant expression of mammalian genes. The advantages of using these hybrid clones of human kidney- and B-cells, called HKBs, for mammalian gene expression, include (i) the cells are negative for immunoglobulin expression, (ii) the cells grow easily in plasma protein-free medium (with or without the addition of recombinant insulin) as suspension cultures in a shake flask or in a fermenter (iii) the cells are very susceptible for transfection of DNA, and (iv) the cells secrete high levels of heterologous recombinant proteins, such as recombinant monoclonal antibodies, soluble ICAM-1, rIL-4, and rFVIII.

Abstract: A method and device for activating and applying a solution of fibrinogen to form fibrin glue at a desired site. The method includes contacting the solution of fibrinogen with immobilized thrombin under conditions that result in a solution of polymerizable fibrin and delivering the activated solution to the desired site. The device includes a housing having a compartment for a solution of fibrinogen, a container for immobilized thrombin, a structure following the solution of fibrinogen to be brought into contact with the immobilized thrombin under conditions which permit the activation of the fibrinogen to polymerizable fibrin, and a structure allowing for delivery of the activated solution to the desired site.

Abstract: Peptides which bind to alpha-1 Proteinase inhibitor (AlPI) are disclosed. The peptides have an available AlPI binding domain which may be any of the following preferred sequences: Val Ile Trp Leu Val Arg, Ile Ile Trp Leu Tyr Lys, Arg Tyr Arg Ile Phe Ile, Arg Ala Phe Trp Tyr Ile, Arg Phe Ile Tyr Tyr Thr, Tyr Lys Phe Arg Phe Trp, Leu Ile Val His Arg Trp, Pro Tyr Trp Ile Val Arg, Ala Arg Trp Tyr Ile His, Gln Tyr His Phe Trp Tyr, Arg Leu Trp Arg Tyr Gly, Val Ile Tyr Leu Val Arg, Val Ile Phe Leu Val Arg, Lys Ile Phe Leu Val Arg, Arg Ile Phe Leu Val Arg, His Ile Phe Leu Val Arg, Arg Val Leu Phe Ile Val, or Arg Val Leu Phe Ile His (SEQ ID NOS: 1-8, 10, 11, 15, 36, 37, 45, 46, 47, 61, and 62 respectively). A method of using peptides having these available binding domains in an affinity chromatography process to purify AlPI is described.

Abstract: Recombinant Factor VIII expression in a mammalian cell culture can be increased by including a novel liposome-like substance in the culture medium. The liposome-like substance comprises at least two (preferably at least three) different lipids in defined molar ratios. In a preferred embodiment, the addition of a liposome-like substance comprised of dioleoyl phosphatidylcholine, phosphatidylethanolamine, and phosphatidylserine in a molar ratio of 4:1:1 to the culture medium of GS-MDR cells resulted in an increase in FVIII production by a factor greater than five.