Patents Represented by Attorney Mintz Levin Cohn Ferris Glovsky and Popeop PC
  • Patent number: 7927797
    Abstract: Embodiments of the present invention are directed to methods and devices/systems for amplifying genetic material and may include providing a water-in-oil emulsion in a continuous flow. The emulsion may include a plurality of water droplets comprising microreactors. Each of the plurality of microreactors may include a single bead capable of capturing a nucleic acid template, a single species nucleic acid template and sufficient reagents to amplify the copy number of the nucleic acid template. The method also includes flowing the emulsion across a first temperature zone and a second lower temperature zone to thermally process the microreactors to amplify the nucleic acid template by polymerase chain reaction.
    Type: Grant
    Filed: January 28, 2005
    Date of Patent: April 19, 2011
    Assignee: 454 Life Sciences Corporation
    Inventors: John R. Nobile, William L. Lee, John H. Leamon