Abstract: A method for the determination of autoantibody in a test sample comprises contacting a substrate for the autoantibody with sample; treating the contacted substrate with labeled antihuman antibody selected from (1) a mixture comprising enzyme labeled antihuman antibody and fluorescent labeled antihuman antibody, and (2) antihuman antibody labeled with an enzyme and a fluorescent label; determining the enzyme activity of the treated substrate; and determining the immunofluorescent patterns in substrates exhibiting enzyme activity. The method is useful for the rapid screening and characterization of autoantibodies.

Abstract: Assay reagents for the determination of enzymes such as .alpha.-amylase, .alpha.-glucosidase, .beta.-glucosidase, and acid and alkaline phosphatases are described. The reagents comprise a substrate capable of releasing the chromophore p-nitrophenol at a rate proportional to the amount of enzyme being assayed and a buffer material that eliminates the temperature dependence of the chromophore.

Abstract: An immunoadsorbent material comprising inactivated, protein-A bearing Staphlococcus aureus immobilized in a polymeric matrix is used to remove IgG immunoglobulins and immune complexes from whole blood.

Abstract: The novel compounds cis-3-hydroxy-4-mercaptotetrahydrothiophene (I) and octahydrodithieno(3,4-b:3',4'e) 1,4-dithin (II) are prepared from 1,4-dithiothreitol and 1,4-dithioerythritol respectively. (I) has utility as an antibacterial agent. (II) has utility as an odorant.

Abstract: An immunoadsorbent material comprising inactivated, protein-A bearing Staphlococcus aureus immobilized in a polymeric matrix is used to remove IgG immunoglobulins and immune complexes from whole blood.

Abstract: Erythrocytes are coated with specific hemagglutinating virus antigen and then treated to produce a stabilized, antigen coated erythrocyte preparation which is used in the determination of antibody in biological fluids.

Abstract: A reagent system for assaying a biological specimen for a peptidase enzyme comprising a peptide substrate in which the C-terminal amino acid is dehydroalanine, the enzyme lactate dehydrogenase, the reduced coenzyme nicotinamide adenine dinucleotide and a buffer material.

Abstract: Addition of a low concentration of an alkali metal metabisulfite completely stops color development in a peroxidase-labeled immunoassay wherein the peroxidase catalyzed reaction comprises oxidation of an achromatic substrate to the corresponding chromatic product in the presence of a peroxide.

Abstract: A method for determining the presence of a ligand in a liquid medium which utilizes enzyme labeled avidin and biotin labeled reagent in a specific binding process wherein the ligand to be detected is contacted with an insoluble phase containing specific binding substance for the ligand. Various protocols may be used to assay the resulting enzyme activity which is related to the amount of ligand in the liquid medium.

Abstract: Disclosed as novel compounds are 3-hydroxy-3-methylglutaric acid monoamide and the lower alkyl esters and salts thereof, 3-hydroxy-3-methylglutaramide mononitrile and 3-hydroxy-3-methylglutaramide. 3-Hydroxy-3-methylglutaric acid monoamide and derivatives thereof exhibit anti-germination and anti-growth utility against a wide variety of perennial and annual weed grasses. 3-Hydroxy-3-methylglutaramide mononitrile and 3-hydroxy-3-methylglutaramide have utility as intermediates in the preparation of 3-hydroxy-3-methylglutaric acid monoamide.

Abstract: A watch pocket for mounting on a belt includes a pair of pliable sheets shaped and secured together to form an upwardly opening pocket and a transverse belt receiving loop behind the pocket with a pair of vertical relief slits formed in the wall between the pocket and loop to provide relief of pressure on the center of the watch and permit the pocket to assume the curvature of the body around which the belt is fastened.

Abstract: A therapeutic composition for treating adrenal cortical carcinoma comprising 1,1-dichloro-2-(o-chlorophenyl)-2-(p-chlorophenyl)ethane and an oil carrier or vehicle.

Abstract: A topical preparation for the treatment of skin diseases such as acne vulgaris, acne rosacea, psoriasis and herpes simplex which comprises 1,1-dichloro-2-(o-chlorophenyl)-2-(p-chlorophenyl)ethane and a pharmaceutically acceptable carrier or diluent.

Abstract: Therapeutic composition useful for mitigating cholelithiasis in humans comprising 3-hydroxy-3-methylglutaric acid, or a pharmaceutically acceptable salt thereof, and a pharmaceutically acceptable non-toxic carrier.

Abstract: Novel 1-[.beta.-(R-thio)phenethyl]-imidazoles and the corresponding 1-[.beta.-(R-sulfinyl)phenethyl]imidazoles and 1-[.beta.-(R-sulfonyl)phenethyl]imidazoles wherein R is alkyl, alkenyl, aralkenyl, substituted aralkenyl, alkynyl, cycloalkyl, cycloalkyl alkyl, aralkyl, substituted aralkyl, aryl or substituted aryl; and the antimicrobial acid addition salts thereof, are useful as antifungal, anti-bacterial and anti-protozoal agents.

Abstract: 3-[3-(1-Methyltetrazol-5-ylthio)-prop-1-(t)-enyl]-7.beta.-(.alpha.-phenylac etamido)-ceph-3-em-4-carboxylic acid; 7.beta.-(.alpha.-phenylacetamido)-3-[3-(1,2,4-triazol-5-ylthio)-prop-1-(t) -enyl]-ceph-3-em-4-carboxylic acid derivatives and salts thereof; and intermediates and processes for preparing such compounds. The compounds are useful as antibacterials and are active against a wide variety of gram positive and gram negative bacteria.

Abstract: 21-ACETALS AND MIXED ACETALS OF STEROIDS OF THE CORTICOID SERIES ARE PREPARED FROM THE CORRESPONDING 21-HYDROXY STEROIDS AND HAVE UTILITY AS ANTI-INFLAMMATORY AGENTS.

Abstract: 21-Esters of 21-aldehyde hydrates and 21-aldehyde hemiacetals of steroids of the corticoid series are prepared from the corresponding 21-hydroxy steroids and have utility as antiinflammatory agents.

Abstract: 3-[3-(1-Methyltetrazol-5-ylthio)-prop-1-(t)-enyl]-7.beta.-(.alpha.-substitu ted acetamido)-ceph-3-em-4-carboxylic acid; 7.beta.-(.alpha.-substituted acetamido)-3-[3-(1,2,4-triazol-5-ylthio)-prop-1-(t)-enyl]-ceph-3-em-4-carb oxylic acid derivatives and salts thereof; and ester intermediates and processes for preparing such compounds. The compounds are useful as antibacterials and are active against a wide variety of gram positive and gram negative bacteria.