Abstract: A method and apparatus for intelligent Internet searching, the apparatus residing as a software application on a user's computer (the client). A single subject database of sources of directly and indirectly accessible content is stored on the client and accessed by the application. The database also necessary information for searching each source. Preferably, the database is updated at a regular interval. Multiple simultaneous hidden database searches may be performed by the application by linking the client to the appropriate database access pages on the network and forwarding the user's desired search information. Preferably, search results are updated and compared to highlight new information.

Abstract: This invention provides a self-assembling polynucleotide delivery system comprising components aiding in the delivery of the polynucleotide to the desired address which are associated via noncovalent interactions with the polynucleotide. The components of this system include DNA-masking components, cell recognition components, charge-neutralization and membrane-permeabilization components, and subcellular localization components. Specific compounds useful in this system are also provided.

Abstract: The invention comprises homogeneous nucleotide amplification strategies and assays. The methods involve amplification of a target nucleic acid sequence that includes the use of a probe that forms a duplex with a target nucleic acid sequence having an enzymatically cleavable region. The probe may anneal to other nucleic acid sequences but only forms an enzymatically cleavable region if the nucleic acid sequence is complementary to the probe. In other embodiments, the probe is configured to act as a primer for the amplification reaction if it anneals to a target nucleic acid sequence and is enzymatically cleaved. The target nucleic acid sequences amplified by the methods of this invention may be assayed by labeling the probe, by employing a second probe having features of the invention or by other suitable methods. Preferably, the probes comprise an RNA portion that forms an RNase H cleavable duplex with DNA.

Abstract: A cigarette lighter adapter formed from two complementary members that form a cylindrically shaped housing having an axial end with an annular recess, and a ring that fits within the annular recess and secures the two complementary portions together. The ring is configured to fit entirely within the annular recess and to fill the recess completely, making removal without destroying the ring or the housing virtually impossible. Preferably, the ring is configured to snap fit into the annular recess. Additional means for securing the two members may also be provided, preferably at a location removed from the first axial end.

Abstract: A composition for transfecting eukaryotic cells comprising a cationic polymer which has protonatable groups which serve to buffer the acidic endosome, protecting the endocytosed polynucleotide from degradation and a viral agent is used to target uptake into and/or lysis from endosomes in the desired eukaryotic cell. By co-infecting the eukaryotic cells with cationic polymer, polynucleotide, and the viral agent, the polynucleotide is brought into the cell and then released. Preferably, polyamidoamine dendrimers are used as the cationic polymer and adenovirus is used as the viral agent. The dendrimers help associate plasmid DNA with the adenovirus, which then provokes receptor-mediated endocytosis. Within the endosome, the tertiary amine groups of the dendrimer buffer the pH change in the endosome. Then, the endosomalytic activity of the adenovirus releases the plasmid DNA into the cell.