Abstract: The present invention provides a method of nucleic acid, including DNA, immunization of a host, including humans, against disease caused by infection by a strain of Chlamydia, specifically C. pneumoniae, employing a vector, containing a nucleotide sequence encoding a CPN100605 polypeptide of a strain of Chlamydia pneumoniae and a promoter to effect expression of the CPN100605 polypeptide in the host. Modifications are possible within the scope of this invention.

Abstract: In summary of this disclosure, the present invention provides a method of nucleic acid, including DNA, immunization of a host, including humans, against disease caused by infection by a strain of Chlamydia, specifically C. pneumoniae, employing a vector, containing a nucleotide sequence encoding an 98 kDa outer membrane protein of a strain of Chlamydia pneumoniae and a promoter to effect expression of the 98 kDa outer membrane protein gene in the host. Modifications are possible within the scope of this invention.

Abstract: In summary of this disclosure, the present invention provides a method of nucleic acid, including DNA, immunization of a host, including humans, against disease caused by infection by a strain of Chlamydia, specifically C. pneumoniae, employing a vector, containing a nucleotide sequence encoding a lorf2 protein of a strain of Chlamydia pneumoniae and a promoter to effect expression of the lorf2 gene in the host. Modifications are possible within the scope of this invention.

Abstract: A method and system are provided for preserving nucleic acids in a bodily fluid, such as urine, blood, blood serum, and amniotic fluid. The preservative includes an amount of a divalent metal chelator selected from ethylenediaminetetraacetic acid (EDTA), [ethylenebis(oxyethylenenitrilo)]tetraacetic acid (EGTA) and 1,2-bis(2-aminophenoxy)ethane-N,N,N′, N′-tetraacetic acid (BAPTA), or salts thereof in the range of from about 0.001M to 0.1M; and an amount of at least one chelator enhancing component selected from lithium chloride, guanidine, sodium salicylate, sodium perchlorate, and sodium thiocyanate in the range of from about 0.1M to 2M.

Abstract: Therapeutic compounds and methods for inhibiting a glycosaminoglycan (GAG)-associated molecular interaction in a subject, whatever its clinical setting, are described.

Abstract: Methods and compounds useful for the inhibition of convulsive disorders, including epilepsy, are disclosed. The methods and compounds of the invention inhibit or prevent ictogenesis and epileptogenesis. Methods for preparing the compounds of the invention are also described.

Abstract: Methods for preparing organic compounds, such as oligonucleotides, on solid supports are described. Libraries of the compounds and methods of using the compounds are also disclosed.

Abstract: Methods for detecting the presence or absence of an analyte in a sample are disclosed. Kits for performing the analysis methods of the invention are also disclosed.

Abstract: Pharmaceuticals and Apparatus used in combination for diagnosis and tissue necrosis applicable to provide effective and selective therapy using the Mossbauer absorption phenomenon. Selected pharmaceutical compounds containing a radiation absorber isotope are administered to a tissue and excited by a radiation source which provides energy at the corresponding resonant Mossbauer absorption frequency of isotope containing pharmaceutical, where excitation effects nuclear transitions to cause highly selective energy absorption in the selected target tissue. For diagnostic purposes, de-excitation fluorescence of the isotope is monitored. For therapeutic purposes, the energy is converted to particle radiation by the isotope at the target tissue by internal conversion followed by an Auger electron cascade which results in radiolysis of DNA resulting in lethal double strand breaks in the DNA molecules of the target tissue.

Abstract: Methods and compositions, useful in the treatment of amyloidosis and conditions and diseases associated therewith, such as Alzheimer's Disease (AD). These methods involve administering to a subject a pharmaceutical composition including one or more agents which modulate APP catabolism and amyloid deposition. Accordingly, the methods and compositions are useful for inhibiting amyloidosis in disorders in which amyloid deposition occurs. The methods are based, at least in part, on modulating catabolism of APP in APP-containing cells through the use of a mobile ionophore, such as carbonyl cyanide p-(trifluoromethoxy)phenylhydrazone, which does not substantially alter the viability of those cells.

Abstract: Methods, compositions, and kits for modulating the stability of at least one nucleic acid duplex, are disclosed.

Abstract: Hydraulic cement set-accelerating admixture compositions; cement compositions containing such admixtures; and methods for preparing said cement compositions. The admixture compositions comprise one or more nitroalcohols present in an amount effective to increase the set acceleration of a hydraulic cement composition. The invention further relates to a method of making a hydraulic cement composition comprising adding to a cement composition comprising a hydraulic cement binder, a nitroalcohol component in an amount effective to increase the set-acceleration of the binder, and to a cement composition comprising a hydraulic cement binder and a nitroalcohol set-accelerating agent.

Abstract: An admixture for cement compositions is provided which contributes both anodic and cathodic corrosion inhibition, and the cement compositions containing such additives. The cement additive is a combination of an anodic corrosion inhibitor such as calcium nitrite, and a cathodic corrosion inhibitor such as calcium stearate or other fatty acid. The mixed inhibitors in accordance with the invention provide as good or better corrosion inhibition than does calcium nitrite when used alone.

Abstract: Doubled-stranded oligonucleotide molecules for use in providing enchaned sequencing of DNA, comprising: a first stand having 1) a priming site; 2) a labeling region contiguous with the priming site, wherein the labeling region consists essentially of at least six identical nucleotides; and 3) a cohesive end contiguous with the labeling region, having a nucleotide sequence complementary to a nucleotide sequence generated by the action of a restriction enzyme; and a second strand annealed to the labeling region of the first stand, but not overlapping with the cohesive end. A method of DNA gene sequencing, involving the use of mobile priming sites for DNA polymerases in DNA sequencing, is also disclosed.

Abstract: A spiral-wound filtration cartridge, which renders the need for brine seals unnecessary, is disclosed. The spiral cartridges of the invention comprise a spirally-wound inner filtration assembly having an impermeable outer cylindrical surface, a housing having a cylindrical cavity formed therein for receiving the inner filtration assembly, and at least one continuous longitudinally-oriented fluid pathway disposed between the inner filtration assembly and the housing, the fluid pathway allowing for a portion of a fluid entering one end of the filtration cartridge to bypass the inner filtration assembly and proceed to the other end of the filtration cartridge.

Abstract: A method for making, clean, bughole-free hardened cement structural units, including the steps of a) applying a release agent to the surface of a form; b) preparing a cement composition; c) adding to the cement composition a water-soluble polymer dispersant and a solution viscosity-reducing agent; d) placing the cement composition of step c) into the form, e) curing the cement composition of step c) to form a hardened structural unit, and f) removing the structural unit from the form.

Abstract: An apparatus for producing shredded aggregate from, e.g., boards of expanded polystyrene, is described. The apparatus includes a shredding box defining a shredding chamber; a rotor housed in the shredding box; means for driving the rotor; a plurality of saw blades coupled to the rotor in linear succession, each saw blade having a plurality of spaced apart teeth, each successive saw blade being coupled to the rotor such that its teeth lie about halfway between the teeth of an adjacent saw blade; receiving means in the shredder box for receiving material to be shredded; and take-away means coupled to the shredder box for removing shredded material from the shredding chamber. Low density (e.g., 0.4-0.5 pcf loose bulk density) material having a uniform particle size distribution over time can be economically produced.

Abstract: The invention relates to a gypsum-based cementitious composition capable of upon mixing with fine aggregate in a weight ratio to said gypsum-based cementitious composition of from about 1.25:1 to 2.5:1; and sufficient water to provide a pumpable, self-leveling wet gypsum-based cementitious composition having a flowability such that the wet gypsum-based cementitious composition completely self-levels to produce a topographically smooth surface without floating or finishing, providing, upon hardening, a sub-floor resistant to delamination from its substrate having a dust-free surface and compressive strength in the range of about 2500 to 6000 psi. The invention further relates to methods for making floor underlayments using these compositions, and for making the compositions.

Abstract: A rehabilitative solution for preventing or retarding the spread of corrosion of metal reinforcements in concrete, comprising a) one or more corrosion-inhibiting agent(s) and b) a impregnation-increasing amount of a penetration-enhancing agent selected from the group consisting of C.sub.1 to C.sub.20 alcohols; alkali metal salts of gluconic acid; alkyl-substituted benzene compounds; or mixtures thereof, is disclosed, along with several methods of its application for concrete rehabilitation.

Abstract: Apparatus and method for dispensing admixtures based upon weight. One or more admixture storage containers are supported in one embodiment by a suspended platform, and in another embodiment by brackets. A weight sensing system such as a single load cell is used for weighing the contents of the admixture storage containers. The platform or brackets are arranged so that the load cell is responsive to less than 100% of the weight in the containers. The containers are filled to a predetermined weight, and the contents are dispensed from each container in series or simultaneously.