Abstract: The present invention concerns new lipid derivatives of phosphonocarboxylic acids of the general formula I, ##STR1## in which the meaning of the symbols is elucidated in the description, tautomers thereof and their physiologically tolerated salts of inorganic and organic bases as well as processes for the production thereof and pharmaceutical agents containing these compounds.
Type:
Grant
Filed:
August 26, 1998
Date of Patent:
September 21, 1999
Assignee:
Roche Diagnostics GmbH
Inventors:
Harald Zilch, Dieter Herrmann, Hans-George Opitz, Gerd Zimmermann, Edgar Voss
Abstract: Method for the specific detection of nucleic acids in a sample by reaction of the sample with one or several labelled nucleotide triphosphates and one or several enzymes which catalyse the production of a labelled nucleic acid B which contains this nucleotide, non-thermally denaturation, reacting the sample with a nucleic acid probe C which is sufficiently complementary to nucleic acid B and which contains at least one immobilizable group, if desired, contacting the nucleic acid hybrid D formed with a solid phase which recognizes and binds the immobilizable group, removing the liquid from the solid phase and determining the label on the solid phase as a measure for the presence of the nucleic acid.
Type:
Grant
Filed:
September 19, 1994
Date of Patent:
May 19, 1998
Assignee:
Boehringer Mannheim GmbH
Inventors:
Christoph Kessler, Rudiger Ruger, Rudolf Seibl, Cornelia Kruse-Muller, Sibylle Berner