Abstract: An apparatus and method for simultaneously loading a plurality of small items such as small items into the wells of a multiple well microtiter plate which includes a small item loading unit and a small item insertion device. The small item loading device includes a top plate having a plurality of openings positioned to register with the microtiter plate wells and a closure unit to close the openings on one side of the top plate. The small item insertion device includes a base mounting a plurality of plunger pins which each register with a well in the microtiter plate. A movable deck unit is mounted in spaced relationship above the base and is provided with apertures, each of which receives a plunger pin. The small item loading device rests on the deck unit and the closure unit opens the openings therein to drop small items into the apertures over the pins. The deck unit is moved toward the base to cause the pins to enter the wells of a microtiter plate and seat the small items.