Patents Represented by Attorney, Agent or Law Firm Norman Kruse
  • Shuffling polynucleotides by incomplete extension
    Patent number: 6506603
    Abstract: A method for DNA reassembly after random fragmentation, and its application to mutagenesis of nucleic acid sequences by in vitro or in vivo recombination is described. In particular, a method for the production of nucleic acid fragments or polynucleotides encoding mutant proteins is described. The present invention also relates to a method of repeated cycles of mutagenesis, shuffling and selection which allow for the directed molecular evolution in vitro or in vivo of proteins.
    Type: Grant
    Filed: November 18, 2000
    Date of Patent: January 14, 2003
    Assignee: Maxygen, Inc.
    Inventor: Willem P. C. Stemmer
  • Methods for generating polynucleotides having desired characteristics by iterative selection and recombination
    Patent number: 6444468
    Abstract: A method for DNA reassembly after random fragmentation, and its application to mutagenesis of nucleic acid sequences by in vitro or in vivo recombination is described. In particular, a method for the production of nucleic acid fragments or polynucleotides encoding mutant proteins is described. The present invention also relates to a method of repeated cycles of mutagenesis, shuffling and selection which allow for the directed molecular evolution in vitro or in vivo of proteins.
    Type: Grant
    Filed: November 28, 2000
    Date of Patent: September 3, 2002
    Assignee: Maxygen, Inc.
    Inventors: Willem P. C. Stemmer, Andreas Crameri
  • Methods for generating polynucleotides having desired characteristics by iterative selection and recombination
    Patent number: 6420175
    Abstract: A method for DNA reassembly after random fragmentation, and its application to mutagenesis of nucleic acid sequences by in vitro or in vivo recombination is described. In particular, a method for the production of nucleic acid fragments or polynucleotides encoding mutant proteins is described. The present invention also relates to a method of repeated cycles of mutagenesis, shuffling and selection which allow for the directed molecular evolution in vitro or in vivo of proteins.
    Type: Grant
    Filed: January 15, 1999
    Date of Patent: July 16, 2002
    Assignee: Maxygen, Inc.
    Inventor: Willem P.C. Stemmer
  • Methods of evolving a polynucleotides by mutagenesis and recombination
    Patent number: 6365408
    Abstract: A method of mutating a polynucleotide such that it has a desired or improved functional property is disclosed.
    Type: Grant
    Filed: January 4, 2000
    Date of Patent: April 2, 2002
    Assignee: Maxygen, Inc.
    Inventor: Willem P. C. Stemmer
  • Methods for generating polynucleotides having desired characteristics by iterative selection and recombination
    Patent number: 6291242
    Abstract: A method for DNA reassembly after random fragmentation, and its application to mutagenesis of nucleic acid sequences by in vitro or in vivo recombination is described. In particular, a method for the production of nucleic acid fragments or polynucleotides encoding mutant proteins is described. The present invention also relates to a method of repeated cycles of mutagenesis, shuffling and selection which allow for the directed molecular evolution in vitro or in vivo of proteins.
    Type: Grant
    Filed: October 2, 1998
    Date of Patent: September 18, 2001
    Assignee: Maxygen, Inc.
    Inventor: Willem P. C. Stemmer
  • Methods for generating polynucleotides having desired characteristics by iterative selection and recombination
    Patent number: 6287861
    Abstract: A method for DNA reassembly after random fragmentation, and its application to mutagenesis of nucleic acid sequences by in vitro or in vivo recombination is described. In particular, a method for the production of nucleic acid fragments or polynucleotides encoding mutant proteins is described. The present invention also relates to a method of repeated cycles of mutagenesis, shuffling and selection which allow for the directed molecular evolution in vitro or in vivo of proteins.
    Type: Grant
    Filed: August 12, 1998
    Date of Patent: September 11, 2001
    Assignee: Maxygen, Inc.
    Inventors: Willem P. C. Stemmer, Andreas Crameri
  • Methods for generating polynucleotides having desired characteristics by iterative selection and recombination
    Patent number: 6277638
    Abstract: A method for DNA reassembly after random fragmentation, and its application to mutagenesis of nucleic acid sequences by in vitro or in vivo recombination is described. In particular, a method for the production of nucleic acid fragments or polynucleotides encoding mutant proteins is described. The present invention also relates to a method of repeated cycles of mutagenesis, shuffling and selection which allow for the directed molecular evolution in vitro or in vivo of proteins.
    Type: Grant
    Filed: January 15, 1999
    Date of Patent: August 21, 2001
    Assignee: Maxygen, Inc.
    Inventor: Willem P. C. Stemmer
  • Methods for generating polynucleotides having desired characteristics by iterative selection and recombination
    Patent number: 6180406
    Abstract: A method for DNA reassembly after random fragmentation, and its application to mutagenesis of nucleic acid sequences by in vitro or in vivo recombination is described. In particular, a method for the production of nucleic acid fragments or polynucleotides encoding mutant proteins is described. The present invention also relates to a method of repeated cycles of mutagenesis, shuffling and selection which allow for the directed molecular evolution in vitro or in vivo of proteins.
    Type: Grant
    Filed: June 17, 1998
    Date of Patent: January 30, 2001
    Assignee: Maxygen, Inc.
    Inventor: Willem P.C. Stemmer
  • Methods for generating polynucleotides having desired characteristics by iterative selection and recombination
    Patent number: 6165793
    Abstract: A method for DNA reassembly after random fragmentation, and its application to mutagenesis of nucleic acid sequences by in vitro or in vivo recombination is described. In particular, a method for the production of nucleic acid fragments or polynucleotides encoding mutant proteins is described. The present invention also relates to a method of repeated cycles of mutagenesis, shuffling and selection which allow for the directed molecular evolution in vitro or in vivo of proteins.
    Type: Grant
    Filed: May 8, 1998
    Date of Patent: December 26, 2000
    Assignee: Maxygen, Inc.
    Inventor: Willem P. C. Stemmer
  • Method for directing evolution of a virus
    Patent number: 6096548
    Abstract: The invention provides a number of strategies for transferring and/or evolving gene(s) associated with cellular DNA uptake so that they confer or enhance DNA-uptake capacity of a recipient cell. Evolution is achieved by recursive cycles of recombination and screening/selection. One such strategy entails evolving genes that confer competence in one species to confer either greater competence in that species, or comparable or greater competence in a second species. Another strategy entails evolving genes for use as components of cloning vector to confer enhanced uptake of the vector. Other strategies entail evolving viral receptors, viruses, and genes that mediate conjugal transfer.
    Type: Grant
    Filed: February 3, 1997
    Date of Patent: August 1, 2000
    Assignee: Maxygen, Inc.
    Inventor: Willem P. C. Stemmer