Abstract: Species-linked diamine triacetic acids of the formula ##STR1## wherein T is an organic species containing at least one amine, hydroxyl, or thiol functional group, L is the residue of at least one of those functional groups and R is a two or more atom long covalent bridge, are disclosed. Methods for their preparation, for the preparation of metal chelates from them and for the use of the chelates are also disclosed. In a preferred embodiment, the metal ions employed in the formation of the chelates are rare earth metal ions capable of forming fluorescent chelates which can in turn be employed in fluoroassay techniques.

Abstract: A port assembly for a container is provided. The port assembly includes an elongated tube extending from an end of a base, the tube defining a tubular bore for receiving a member for accessing the container. The tubular bore includes a membrane for separating the tubular bore into a first portion and second portion, the first portion being closer to the container than the second portion. A resealing injection site is mechanically locked within the second portion of the channel. A method for making the port assembly is also provided.

Abstract: A port and closure assembly is provided. The closure includes a sleeve member and the port a rib member. The sleeve member having an inner circumference that is less than the outer circumference of the rib. The port and closure cooperating to produce an audible signal when the closure is removed from the port.

Abstract: A system for heat sealing in conjunction a pair of plastic strips each of having a series of regularly spaced transverse pockets so that the pockets together form chambers separated by webs. The two pocketed strips to be joined are brought together and the strips heat sealed around the pockets to seal the chambers from one another while passing cooling fluid over the pockets so as to avoid distortion or collapse of the pockets.

Abstract: A method for conducting a ligand assay in an inert porous medium wherein a binding material is immunologically immobilized within the medium, which includes the steps of immunologically immobilizing a binding material within a finite zone of the medium, applying an analyte to the zone containing the immobilized binding material, applying a labeled indicator to the zone which becomes immobilized within the zone in an amount which can be correlated to the amount of analyte in the zone, applying a solvent to substantially the center of the zone to chromatographically separate the unbound labeled indicator from the zone, and measuring the amount of labeled indicator remaining in the zone.

Abstract: A method and assembly provided for the detection and determination of at least one ligand in a sample suspected of containing the ligand. The method includes providing in a hand-held assembly having a first chamber and a second chamber separated by a separation means which permits the passage therethrough of materials of a relatively small size while preventing the passage therethrough of materials of a relatively larger size; a suspension in the first chamber of an admixture of a binding partner for the ligand and a labeled component. The labeled component is selected from the group consisting of binding partner, compound which can bind to the binding partner, compound which can bind to the reaction product of the ligand and binding partner, and compound which can bind to the ligand. The sample suspected of containing the ligand is introduced to the first chamber.

Abstract: A method for conducting a ligand assay in an inert porous medium wherein a binding material is immunologically immobilized within the medium, which includes the steps of immunologically immobilizing a binding material within a finite zone of the medium, applying an analyte to the zone containing the immobilized binding material, applying a labeled indicator to the zone which becomes immobilized within the zone in an amount which can be correlated to the amount of analyte in the zone, applying a solvent to substantially the center of the zone to chromatographically separate the unbound labeled indicator from the zone, and measuring the amount of labeled indicator remaining in the zone.

Abstract: It is disclosed that aryl-substituted 2,6-bis[N,N-di(carboxyalkyl)aminoalkyl] pyridines in which the aryl groups are substituted with one or more electron-releasing groups are an advantageous ligand for forming fluorescent chelates with rare earth metals. The pyridine moieties can be linked to target molecules, especially biologically active target molecules to provide fluorescent tagging for use in fluoroassay techniques. The pyridine moieties are disclosed as tetraacids, as salts and as esters. Preparation processes and precursors including the corresponding aryl-substituted 2,6-dicarboxypyridines as acids, salts and esters, are disclosed as well.

Abstract: Disclosed is a method for detection for serum antibody and/or microbial surface antigen by radial partition immunoassay. The method of this invention is applicable to (a) the evaluation of a clinical specimen for identification of a microorganism; (b) antimicrobial sensitivity assays for determination of an efficacious antibiotic for use against a specific microorganism; (c) a semi-quantitative determination of viral surface antigen; and, (d) a quantitative method for the determination of the presence of serum antibodies to microbial antigens. In each of the foregoing applications, the analyte of interest can be immobilized within a porous matrix (solid phase) by simple pipetting of the sample onto the prepared matrix. Appropriate reagents are subsequently applied to the matrix to effect immunochemical interaction of a labeled binding material to the surface antigen (or antibody) of the analyte of interest. The portion of the matrix within which such interaction takes place is termed the "reaction zone".

Abstract: A specimen tray assembly for use in an automatic analyzing system for analyzing a plurality of specimen within the tray. The assembly includes a container tray having a plurality of microcuvettes arranged in a spaced apart grid-like pattern. A cover member is adapted to seat over the top surface of the container tray, the cover member including a system for automatically centering the container tray relative to the cover member to provide proper seating of the cover member on the container tray.

Abstract: A multilayered tube (10) is provided that includes an outer layer (12) comprising ethylene-vinyl acetate for bonding to an ethylene-vinyl acetate material and an inner layer (14) comprising polyvinyl chloride for bonding to polyvinyl chloride material. A bonding layer (16) is provided between the outer and inner layers for bonding and preventing delamination of the outer and inner layers.In another embodiment, a port and membrane tube assembly (18) is provided that includes the above-described multilayered tube and a polyvinyl chloride membrane tube bonded to the inner layer of the multilayered tube.The multilayered tube or the port and membrane tube assembly can be bonded to an ethylene-vinyl acetate container which can be used to store various types of solutions useful in medical treatment.

Abstract: Gamma globulin concentrates are stabilized against anticomplement activity generation during lyophilization by inclusion in the concentrate of a physiologically acceptable, substantially nonsurface-active hydrophilic macromolecule. The improved concentrates may be safely administered to patients. The main additives to the gamma globulin are a substantially nonsurface-active hydrophilic macromolecule, a protein and a polyol.

Abstract: A tamper indicating device (24) is connected to the inlet (14) of an injection site (10) which inlet (14) includes a pierceable, self-sealing member (16). The tamper indicating device (24) comprises a cap (26, 28) which covers the inlet (14) rendering the pierceable, self-sealing member (16) inaccessible. A first portion (26) of the cap is connected to the inlet (14) and a second portion (28) of the cap is separated from the first portion (26) by a frangible section (30). A handle (32) extends from the second portion (28) and is operable when actuated to break the second portion (28) away from the first portion (26) at the frangible section (30), rendering the pierceable, self-sealing member (16) accessible. The first portion (26) remains connected to the inlet (14) to indicate that tampering has occurred.

Abstract: A system for infusing fluids into the human body includes a peristaltic-type metering apparatus which controls the flow of fluid through a disposable administration set at a preset rate. A compensating circuit within the metering apparatus automatically varies the operating rate of the metering apparatus to compensate for dimensional variations in the apparatus and the tubing.

Abstract: A flexible, flat-collapsible bag containing parenteral solution may be adapted for aseptically receiving and mixing the contents of another container, with the flat-collapsible bag preferably initially containing, prior to receiving said contents, a liquid for parenteral administration in a volume of 30 to 70 percent of the holding capacity of said flat-collapsible bag. The bag defines at least a pair of access ports, one of the ports being sealingly connected to flexible, crimpable, thermoplastic tubing of a length of at least 11/2 inches, and a sealed connector member carried on the outer end of the flexible thermoplastic tubing, in which the seal connector member defines a sleeve including a sealing diaphragm in the bore thereof, with the sleeve constituting an extension of the flexible thermoplastic tubing. Accordingly, a piercing spike may sealingly enter the sleeve to rupture the diaphragm.

Abstract: The cuvette rotor assembly is adapted for use in a multistation photometric analyzer of the type wherein several chemical reactions are sequentially monitored over a predetermined time span. Such an assembly includes a ring shaped cuvette rotor having an outer circular periphery, an inner circular periphery, a first side, a second side and cuvette forming slots extending between the sides and into the rotor from the inner periphery toward the outer periphery. A mixture of reagent and sample is urged by centrifugal force into the cuvette forming slots as the rotor is rotated. A fixed beam of light is directed at one side of the rotor as the rotor is rotating and the light that passes through each cuvette as a reaction is taking place therein is sensed and measured.