Abstract: A method of purifying granulocyte-macrophage colony-stimulating factor (GM-CSF), particularly recombinant human GM-CSF, in good yield and with retention of biological activity, comprising sequential anion-exchange, dye-ligand affinity, gel filtration and reversed-phase chromatography is disclosed.
Type:
Grant
Filed:
September 21, 1993
Date of Patent:
February 21, 1995
Assignee:
Schering Plough Corporation
Inventors:
Paul P. Trotta, Gail F. Seelig, Robert A. Kosecki, Paul Reichert
Abstract: Disclosed is an improved microcentrifuge tube (10). One improvement is a recessed well (20) within the top of the the lid (16) of the tube into which an identifying disk (22) is placed. Another claimed improvement is an expanded lower circumference (25) of the lower portion of plug (24) extending down from the bottom of the top (16) of the tube and an inwardly directed protrusion (26) on the inner surface of the top portion of the tube such that the expanded circumference (25) at the lower portion of the plug (24) fits under the inwardly directed protrusion (26) when said plug is inserted into the opening of the tube thus securing the lid to the top of the tube.
Type:
Grant
Filed:
October 19, 1992
Date of Patent:
October 19, 1993
Assignee:
International Mould Engineering
Inventors:
Zhilong Yu, Zhipeng Yu, Perry B. Newton, III
Abstract: A method for lowering the blood-serum cholesterol levels in mammals by administering IL-4 is disclosed as well as the use of IL-4 for the manufacture of a medicament for use in lowering blood-serum cholesterol levels.